Abstract
Surfactant protein (SP)-A and SP-D are collagen-like glycoproteins that are synthesized in the distal pulmonary epithelium. This study examined the effects of ageing and long-term smoking on SP-A and SP-D in the lungs. The possible links to the development of pulmonary emphysema were also investigated. Sequential lavage was performed in young and middle-aged or elderly nonsmokers and asymptomatic current smokers with various smoking histories. Middle-aged or elderly smokers were further categorised according to the presence of emphysema by high-resolution computed tomography. Levels of SP-A and SP-D in bronchial lavage (BL) fluid and in bronchoalveolar lavage (BAL) fluid were quantified by ELISA. Significant decreases in SP-A were seen with age in nonsmokers in BL fluid, but not in BAL fluid. Middle-aged or elderly smokers with emphysema had lower levels of SP-A in both BL and BAL fluids when compared with young subjects, and in BL fluid when compared with middle-aged or elderly smokers without emphysema. SP-D did not change with age alone, however, it was decreased in middle-aged or elderly smokers when compared with similarly aged nonsmokers. In conclusion, surfactant protein-A may decrease with age alone or due to the cumulative effects of long-term smoking and development of emphysema, while surfactant protein-D decreases due to long-term smoking.
| Original language | English |
|---|---|
| Pages (from-to) | 964-970 |
| Number of pages | 7 |
| Journal | European Respiratory Journal |
| Volume | 24 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2004 Dec |
| Externally published | Yes |
Fingerprint
Keywords
- Ageing
- Chronic obstructive pulmonary disease
- Collectin
- Pulmonary mephysema
- Sequential bronchoalveolar lavage
- Smoking
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
Effects of ageing and smoking on SP-A and SP-D levels in bronchoalveolar lavage fluid. / Betsuyaku, Tomoko; Kuroki, Y.; Nagai, K.; Nasuhara, Y.; Nishimura, M.
In: European Respiratory Journal, Vol. 24, No. 6, 12.2004, p. 964-970.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of ageing and smoking on SP-A and SP-D levels in bronchoalveolar lavage fluid
AU - Betsuyaku, Tomoko
AU - Kuroki, Y.
AU - Nagai, K.
AU - Nasuhara, Y.
AU - Nishimura, M.
PY - 2004/12
Y1 - 2004/12
N2 - Surfactant protein (SP)-A and SP-D are collagen-like glycoproteins that are synthesized in the distal pulmonary epithelium. This study examined the effects of ageing and long-term smoking on SP-A and SP-D in the lungs. The possible links to the development of pulmonary emphysema were also investigated. Sequential lavage was performed in young and middle-aged or elderly nonsmokers and asymptomatic current smokers with various smoking histories. Middle-aged or elderly smokers were further categorised according to the presence of emphysema by high-resolution computed tomography. Levels of SP-A and SP-D in bronchial lavage (BL) fluid and in bronchoalveolar lavage (BAL) fluid were quantified by ELISA. Significant decreases in SP-A were seen with age in nonsmokers in BL fluid, but not in BAL fluid. Middle-aged or elderly smokers with emphysema had lower levels of SP-A in both BL and BAL fluids when compared with young subjects, and in BL fluid when compared with middle-aged or elderly smokers without emphysema. SP-D did not change with age alone, however, it was decreased in middle-aged or elderly smokers when compared with similarly aged nonsmokers. In conclusion, surfactant protein-A may decrease with age alone or due to the cumulative effects of long-term smoking and development of emphysema, while surfactant protein-D decreases due to long-term smoking.
AB - Surfactant protein (SP)-A and SP-D are collagen-like glycoproteins that are synthesized in the distal pulmonary epithelium. This study examined the effects of ageing and long-term smoking on SP-A and SP-D in the lungs. The possible links to the development of pulmonary emphysema were also investigated. Sequential lavage was performed in young and middle-aged or elderly nonsmokers and asymptomatic current smokers with various smoking histories. Middle-aged or elderly smokers were further categorised according to the presence of emphysema by high-resolution computed tomography. Levels of SP-A and SP-D in bronchial lavage (BL) fluid and in bronchoalveolar lavage (BAL) fluid were quantified by ELISA. Significant decreases in SP-A were seen with age in nonsmokers in BL fluid, but not in BAL fluid. Middle-aged or elderly smokers with emphysema had lower levels of SP-A in both BL and BAL fluids when compared with young subjects, and in BL fluid when compared with middle-aged or elderly smokers without emphysema. SP-D did not change with age alone, however, it was decreased in middle-aged or elderly smokers when compared with similarly aged nonsmokers. In conclusion, surfactant protein-A may decrease with age alone or due to the cumulative effects of long-term smoking and development of emphysema, while surfactant protein-D decreases due to long-term smoking.
KW - Ageing
KW - Chronic obstructive pulmonary disease
KW - Collectin
KW - Pulmonary mephysema
KW - Sequential bronchoalveolar lavage
KW - Smoking
UR - http://www.scopus.com/inward/record.url?scp=10444246636&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10444246636&partnerID=8YFLogxK
U2 - 10.1183/09031936.04.00064004
DO - 10.1183/09031936.04.00064004
M3 - Article
C2 - 15572540
AN - SCOPUS:10444246636
VL - 24
SP - 964
EP - 970
JO - European Respiratory Journal
JF - European Respiratory Journal
SN - 0903-1936
IS - 6
ER -