Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary

Alexei A. Sharov, Geppino Falco, Yulan Piao, Suresh Poosala, Kevin G. Becker, Alan B. Zonderman, Dan L. Longo, David Schlessinger, Minoru Ko

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background: The aging of reproductive organs is not only a major social issue, but of special interest in aging research. A long-standing view of 'immortal germ line versus mortal soma' poses an important question of whether the reproductive tissues age in similar ways to the somatic tissues. As a first step to understand this phenomenon, we examine global changes in gene expression patterns by DNA microarrays in ovaries and testes of C57BL/6 mice at 1, 6, 16, and 24 months of age. In addition, we compared a group of mice on ad libitum (AL) feeding with a group on lifespan-extending 40% calorie restriction (CR). Results: We found that gene expression changes occurred in aging gonads, but were generally different from those in somatic organs during aging. For example, only two functional categories of genes previously associated with aging in muscle, kidney, and brain were confirmed in ovary: genes associated with complement activation were upregulated, and genes associated with mitochondrial electron transport were downregulated. The bulk of the changes in gonads were mostly related to gonad-specific functions. Ovaries showed extensive gene expression changes with age, especially in the period when ovulation ceases (from 6 to 16 months), whereas testes showed only limited age-related changes. The same trend was seen for the effects of CR: CR-mediated reversal of age-associated gene expression changes, reported in somatic organs previously, was limited to a small number of genes in gonads. Instead, in both ovary and testis, CR caused small and mostly gonad-specific effects: suppression of ovulation in ovary and activation of testis-specific genes in testis. Conclusion: Overall, the results are consistent with unique modes of aging and its modification by CR in testis and ovary.

Original languageEnglish
Article number24
JournalBMC Biology
Volume6
DOIs
Publication statusPublished - 2008 Jun 3
Externally publishedYes

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Transcriptome
Gene expression
Gonads
gene expression
Testis
gonads
Ovary
testes
Aging of materials
Genes
mice
gene
Gene Expression
genes
ovulation
Chemical activation
Ovulation Inhibition
Tissue
ad libitum feeding
Complement Activation

ASJC Scopus subject areas

  • Biotechnology
  • Structural Biology
  • Developmental Biology
  • Physiology
  • Plant Science
  • Cell Biology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary. / Sharov, Alexei A.; Falco, Geppino; Piao, Yulan; Poosala, Suresh; Becker, Kevin G.; Zonderman, Alan B.; Longo, Dan L.; Schlessinger, David; Ko, Minoru.

In: BMC Biology, Vol. 6, 24, 03.06.2008.

Research output: Contribution to journalArticle

Sharov, AA, Falco, G, Piao, Y, Poosala, S, Becker, KG, Zonderman, AB, Longo, DL, Schlessinger, D & Ko, M 2008, 'Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary', BMC Biology, vol. 6, 24. https://doi.org/10.1186/1741-7007-6-24
Sharov, Alexei A. ; Falco, Geppino ; Piao, Yulan ; Poosala, Suresh ; Becker, Kevin G. ; Zonderman, Alan B. ; Longo, Dan L. ; Schlessinger, David ; Ko, Minoru. / Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary. In: BMC Biology. 2008 ; Vol. 6.
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AB - Background: The aging of reproductive organs is not only a major social issue, but of special interest in aging research. A long-standing view of 'immortal germ line versus mortal soma' poses an important question of whether the reproductive tissues age in similar ways to the somatic tissues. As a first step to understand this phenomenon, we examine global changes in gene expression patterns by DNA microarrays in ovaries and testes of C57BL/6 mice at 1, 6, 16, and 24 months of age. In addition, we compared a group of mice on ad libitum (AL) feeding with a group on lifespan-extending 40% calorie restriction (CR). Results: We found that gene expression changes occurred in aging gonads, but were generally different from those in somatic organs during aging. For example, only two functional categories of genes previously associated with aging in muscle, kidney, and brain were confirmed in ovary: genes associated with complement activation were upregulated, and genes associated with mitochondrial electron transport were downregulated. The bulk of the changes in gonads were mostly related to gonad-specific functions. Ovaries showed extensive gene expression changes with age, especially in the period when ovulation ceases (from 6 to 16 months), whereas testes showed only limited age-related changes. The same trend was seen for the effects of CR: CR-mediated reversal of age-associated gene expression changes, reported in somatic organs previously, was limited to a small number of genes in gonads. Instead, in both ovary and testis, CR caused small and mostly gonad-specific effects: suppression of ovulation in ovary and activation of testis-specific genes in testis. Conclusion: Overall, the results are consistent with unique modes of aging and its modification by CR in testis and ovary.

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