Effects of air and irrigating solutions on the corneal endothelium

Kazuo Tsubota, R. A. Laing, K. Chiba, K. R. Kenyon

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

It is well established that the corneal endothelium reflects the trauma it experiences by changing its morphological appearance. We have studied the effect of mild trauma to the rabbit endothelium by replacing the aqueous humor with air. After either 2 or 4 h, the air was removed and then replaced with either physiological saline or balanced salt solution (BSS). Specular microscopy was then performed for 1 week to observe endothelial changes. Various transient morphological changes observed during this period included many nonhexagonal cells, enlarged cells, and clusters of small cells. The changes produced were more dramatic for the saline replacement group than the BSS replacement group, indicating that the recovery process of the endothelium following this mild trauma depends on the material used to replace the air. The air appears to sensitize the endothelium to subsequent insult. This model can perhaps be used to evaluate the relative safety of the various solutions that are used in the anterior chamber during ocular surgery.

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalCornea
Volume7
Issue number2
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Corneal Endothelium
Air
Endothelium
Wounds and Injuries
Salts
Aqueous Humor
Anterior Chamber
Microscopy
Rabbits
Safety

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Tsubota, K., Laing, R. A., Chiba, K., & Kenyon, K. R. (1988). Effects of air and irrigating solutions on the corneal endothelium. Cornea, 7(2), 115-121.

Effects of air and irrigating solutions on the corneal endothelium. / Tsubota, Kazuo; Laing, R. A.; Chiba, K.; Kenyon, K. R.

In: Cornea, Vol. 7, No. 2, 1988, p. 115-121.

Research output: Contribution to journalArticle

Tsubota, K, Laing, RA, Chiba, K & Kenyon, KR 1988, 'Effects of air and irrigating solutions on the corneal endothelium', Cornea, vol. 7, no. 2, pp. 115-121.
Tsubota K, Laing RA, Chiba K, Kenyon KR. Effects of air and irrigating solutions on the corneal endothelium. Cornea. 1988;7(2):115-121.
Tsubota, Kazuo ; Laing, R. A. ; Chiba, K. ; Kenyon, K. R. / Effects of air and irrigating solutions on the corneal endothelium. In: Cornea. 1988 ; Vol. 7, No. 2. pp. 115-121.
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