Effects of (+)-, (-)- and (±)-indenestrols A and B on microtubule distribution and cytotoxicity in Chinese hamster V79 cells

Taiko Oda, Yumiko Sakakibara, Ken Ichinoseki, Eriko Yokota, Yoshihiro Sato

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have reported that (+)-, (-)- and (±)-indenestrols A and B (IA and IB respectively) inhibit the polymerization of microtubule proteins isolated from porcine brain in vitro. In this study, the effects of (+)-, (-)- and (±)-IA and IB on the relative plating efficiency, chromosome number and cellular microtubular architecture of Chinese hamster V79 cells, detected with a fluorescent anti-tubulin antibody, were investigated. The results indicated that the effect of (±)-IA was similar to that of diethylstilbestrol and that of (±)-IB was greater than that of (±)-IA. We also determined the effects of the optically active IA and IB isomers and found that the rank order of cytotoxic activity of the IA and IB series was: (-)-IA>(±)-IA>(+)-IA and (±)-IB≧(-)-IB>(+)-IB. Furthermore, we studied the intracellular disturbance of microtubule formation induced by these compounds and found that (-)-IA had by far the greatest disruptive effect.

Original languageEnglish
Pages (from-to)223-230
Number of pages8
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume289
Issue number2
DOIs
Publication statusPublished - 1993

Fingerprint

Microtubule Proteins
Diethylstilbestrol
Tubulin
Cricetulus
Microtubules
Polymerization
Anti-Idiotypic Antibodies
Swine
Chromosomes
Brain
In Vitro Techniques
indenestrol
indenestrol B

Keywords

  • Aneuploidy
  • Cytotoxicity
  • Immunofluorescence
  • Indenestrol A
  • Indenestrol B
  • Microtubule assembly

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis

Cite this

Effects of (+)-, (-)- and (±)-indenestrols A and B on microtubule distribution and cytotoxicity in Chinese hamster V79 cells. / Oda, Taiko; Sakakibara, Yumiko; Ichinoseki, Ken; Yokota, Eriko; Sato, Yoshihiro.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 289, No. 2, 1993, p. 223-230.

Research output: Contribution to journalArticle

@article{81e1609a8c7940a89902ff164174ff89,
title = "Effects of (+)-, (-)- and (±)-indenestrols A and B on microtubule distribution and cytotoxicity in Chinese hamster V79 cells",
abstract = "We have reported that (+)-, (-)- and (±)-indenestrols A and B (IA and IB respectively) inhibit the polymerization of microtubule proteins isolated from porcine brain in vitro. In this study, the effects of (+)-, (-)- and (±)-IA and IB on the relative plating efficiency, chromosome number and cellular microtubular architecture of Chinese hamster V79 cells, detected with a fluorescent anti-tubulin antibody, were investigated. The results indicated that the effect of (±)-IA was similar to that of diethylstilbestrol and that of (±)-IB was greater than that of (±)-IA. We also determined the effects of the optically active IA and IB isomers and found that the rank order of cytotoxic activity of the IA and IB series was: (-)-IA>(±)-IA>(+)-IA and (±)-IB≧(-)-IB>(+)-IB. Furthermore, we studied the intracellular disturbance of microtubule formation induced by these compounds and found that (-)-IA had by far the greatest disruptive effect.",
keywords = "Aneuploidy, Cytotoxicity, Immunofluorescence, Indenestrol A, Indenestrol B, Microtubule assembly",
author = "Taiko Oda and Yumiko Sakakibara and Ken Ichinoseki and Eriko Yokota and Yoshihiro Sato",
year = "1993",
doi = "10.1016/0027-5107(93)90073-O",
language = "English",
volume = "289",
pages = "223--230",
journal = "Mutation Research",
issn = "0027-5107",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Effects of (+)-, (-)- and (±)-indenestrols A and B on microtubule distribution and cytotoxicity in Chinese hamster V79 cells

AU - Oda, Taiko

AU - Sakakibara, Yumiko

AU - Ichinoseki, Ken

AU - Yokota, Eriko

AU - Sato, Yoshihiro

PY - 1993

Y1 - 1993

N2 - We have reported that (+)-, (-)- and (±)-indenestrols A and B (IA and IB respectively) inhibit the polymerization of microtubule proteins isolated from porcine brain in vitro. In this study, the effects of (+)-, (-)- and (±)-IA and IB on the relative plating efficiency, chromosome number and cellular microtubular architecture of Chinese hamster V79 cells, detected with a fluorescent anti-tubulin antibody, were investigated. The results indicated that the effect of (±)-IA was similar to that of diethylstilbestrol and that of (±)-IB was greater than that of (±)-IA. We also determined the effects of the optically active IA and IB isomers and found that the rank order of cytotoxic activity of the IA and IB series was: (-)-IA>(±)-IA>(+)-IA and (±)-IB≧(-)-IB>(+)-IB. Furthermore, we studied the intracellular disturbance of microtubule formation induced by these compounds and found that (-)-IA had by far the greatest disruptive effect.

AB - We have reported that (+)-, (-)- and (±)-indenestrols A and B (IA and IB respectively) inhibit the polymerization of microtubule proteins isolated from porcine brain in vitro. In this study, the effects of (+)-, (-)- and (±)-IA and IB on the relative plating efficiency, chromosome number and cellular microtubular architecture of Chinese hamster V79 cells, detected with a fluorescent anti-tubulin antibody, were investigated. The results indicated that the effect of (±)-IA was similar to that of diethylstilbestrol and that of (±)-IB was greater than that of (±)-IA. We also determined the effects of the optically active IA and IB isomers and found that the rank order of cytotoxic activity of the IA and IB series was: (-)-IA>(±)-IA>(+)-IA and (±)-IB≧(-)-IB>(+)-IB. Furthermore, we studied the intracellular disturbance of microtubule formation induced by these compounds and found that (-)-IA had by far the greatest disruptive effect.

KW - Aneuploidy

KW - Cytotoxicity

KW - Immunofluorescence

KW - Indenestrol A

KW - Indenestrol B

KW - Microtubule assembly

UR - http://www.scopus.com/inward/record.url?scp=0027376949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027376949&partnerID=8YFLogxK

U2 - 10.1016/0027-5107(93)90073-O

DO - 10.1016/0027-5107(93)90073-O

M3 - Article

C2 - 7690891

AN - SCOPUS:0027376949

VL - 289

SP - 223

EP - 230

JO - Mutation Research

JF - Mutation Research

SN - 0027-5107

IS - 2

ER -