Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis

Asao Makioka, Hiroshi Ohtomo, Seiki Kobayashi, Tsutomu Takeuchi

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4 Citations (Scopus)

Abstract

We have detected and characterized DNA polymerase activity in cell extracts from trophozoites of Entamoeba histolytica and have found that the activity of E. histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative DNA polymerases. The present study was aimed to evaluate the effect of aphidicolin on growth and DNA synthesis by this parasite. Aphidicolin blocked the growth of axenic E. histolytica strain HM-1: IMSS. DNA synthesis was also inhibited by aphidicolin when assayed by incorporation of [3H] thymidine into the DNA. The inhibitory effect of aphidicolin on the growth of E. histolytica was abrogated by removal of the drug, and exposure to 3μg/ml of the drug for at least 48 hr had little effect on the viability. Synchronous growth was observed in the recovery phase after removal of aphidicolin.

Original languageEnglish
Pages (from-to)417-422
Number of pages6
JournalTokai Journal of Experimental and Clinical Medicine
Volume23
Issue number6
Publication statusPublished - 1998 Dec 1

Keywords

  • Aphidicolin
  • DNA polymerase
  • DNA synthesis
  • Entamoeba histolytica
  • Growth
  • Protozoa

ASJC Scopus subject areas

  • Medicine(all)

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    Makioka, A., Ohtomo, H., Kobayashi, S., & Takeuchi, T. (1998). Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis. Tokai Journal of Experimental and Clinical Medicine, 23(6), 417-422.