Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis

Asao Makioka, Hiroshi Ohtomo, Seiki Kobayashi, Tsutomu Takeuchi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have previously demonstrated that DNA polymerase activity of Entamoeba histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative DNA polymerases. The present study was aimed to evaluate the effect of aphidicolin on growth and DNA synthesis by this parasite. Aphidicolin blocked the growth of axenic E. histolytica strain HM-1:IMSS. DNA synthesis was also inhibited by aphidicolin when assayed by incorporation of [3H]thymidine into the DNA. The inhibitory effect of aphidicolin on the growth of E. histolytica was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 48 hr had little effect on the viability. Synchronous growth was observed in the recovery phase after removal of aphidicolin.

Original languageEnglish
Pages (from-to)857-859
Number of pages3
JournalJournal of Parasitology
Volume84
Issue number4
DOIs
Publication statusPublished - 1998 Aug

Fingerprint

Aphidicolin
Entamoeba histolytica
DNA-directed DNA polymerase
DNA
synthesis
Growth
drugs
DNA-Directed DNA Polymerase
thymidine
drug
nuclear genome
viability
parasites
inhibitor
parasite
Pharmaceutical Preparations
Thymidine
effect
Parasites

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis. / Makioka, Asao; Ohtomo, Hiroshi; Kobayashi, Seiki; Takeuchi, Tsutomu.

In: Journal of Parasitology, Vol. 84, No. 4, 08.1998, p. 857-859.

Research output: Contribution to journalArticle

Makioka, A, Ohtomo, H, Kobayashi, S & Takeuchi, T 1998, 'Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis', Journal of Parasitology, vol. 84, no. 4, pp. 857-859. https://doi.org/10.2307/3284604
Makioka, Asao ; Ohtomo, Hiroshi ; Kobayashi, Seiki ; Takeuchi, Tsutomu. / Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis. In: Journal of Parasitology. 1998 ; Vol. 84, No. 4. pp. 857-859.
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