Abstract
We have previously demonstrated that DNA polymerase activity of Entamoeba histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative DNA polymerases. The present study was aimed to evaluate the effect of aphidicolin on growth and DNA synthesis by this parasite. Aphidicolin blocked the growth of axenic E. histolytica strain HM-1:IMSS. DNA synthesis was also inhibited by aphidicolin when assayed by incorporation of [3H]thymidine into the DNA. The inhibitory effect of aphidicolin on the growth of E. histolytica was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 48 hr had little effect on the viability. Synchronous growth was observed in the recovery phase after removal of aphidicolin.
| Original language | English |
|---|---|
| Pages (from-to) | 857-859 |
| Number of pages | 3 |
| Journal | Journal of Parasitology |
| Volume | 84 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1998 Aug |
Fingerprint
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Parasitology
- Microbiology
Cite this
Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis. / Makioka, Asao; Ohtomo, Hiroshi; Kobayashi, Seiki; Takeuchi, Tsutomu.
In: Journal of Parasitology, Vol. 84, No. 4, 08.1998, p. 857-859.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis
AU - Makioka, Asao
AU - Ohtomo, Hiroshi
AU - Kobayashi, Seiki
AU - Takeuchi, Tsutomu
PY - 1998/8
Y1 - 1998/8
N2 - We have previously demonstrated that DNA polymerase activity of Entamoeba histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative DNA polymerases. The present study was aimed to evaluate the effect of aphidicolin on growth and DNA synthesis by this parasite. Aphidicolin blocked the growth of axenic E. histolytica strain HM-1:IMSS. DNA synthesis was also inhibited by aphidicolin when assayed by incorporation of [3H]thymidine into the DNA. The inhibitory effect of aphidicolin on the growth of E. histolytica was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 48 hr had little effect on the viability. Synchronous growth was observed in the recovery phase after removal of aphidicolin.
AB - We have previously demonstrated that DNA polymerase activity of Entamoeba histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative DNA polymerases. The present study was aimed to evaluate the effect of aphidicolin on growth and DNA synthesis by this parasite. Aphidicolin blocked the growth of axenic E. histolytica strain HM-1:IMSS. DNA synthesis was also inhibited by aphidicolin when assayed by incorporation of [3H]thymidine into the DNA. The inhibitory effect of aphidicolin on the growth of E. histolytica was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 48 hr had little effect on the viability. Synchronous growth was observed in the recovery phase after removal of aphidicolin.
UR - http://www.scopus.com/inward/record.url?scp=0031820798&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031820798&partnerID=8YFLogxK
U2 - 10.2307/3284604
DO - 10.2307/3284604
M3 - Article
C2 - 9714225
AN - SCOPUS:0031820798
VL - 84
SP - 857
EP - 859
JO - Journal of Parasitology
JF - Journal of Parasitology
SN - 0022-3395
IS - 4
ER -