Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis

Asao Makioka; Hiroshi Ohtomo; Seiki Kobayashi; Tsutomu Takeuchi

doi:10.2307/3284604

Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis

Asao Makioka, Hiroshi Ohtomo, Seiki Kobayashi, Tsutomu Takeuchi

Department of Infectious Diseases

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We have previously demonstrated that DNA polymerase activity of Entamoeba histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative DNA polymerases. The present study was aimed to evaluate the effect of aphidicolin on growth and DNA synthesis by this parasite. Aphidicolin blocked the growth of axenic E. histolytica strain HM-1:IMSS. DNA synthesis was also inhibited by aphidicolin when assayed by incorporation of [³H]thymidine into the DNA. The inhibitory effect of aphidicolin on the growth of E. histolytica was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 48 hr had little effect on the viability. Synchronous growth was observed in the recovery phase after removal of aphidicolin.

Original language	English
Pages (from-to)	857-859
Number of pages	3
Journal	Journal of Parasitology
Volume	84
Issue number	4
DOIs	https://doi.org/10.2307/3284604
Publication status	Published - 1998 Aug

ASJC Scopus subject areas

Parasitology
Ecology, Evolution, Behavior and Systematics

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title = "Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis",

abstract = "We have previously demonstrated that DNA polymerase activity of Entamoeba histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative DNA polymerases. The present study was aimed to evaluate the effect of aphidicolin on growth and DNA synthesis by this parasite. Aphidicolin blocked the growth of axenic E. histolytica strain HM-1:IMSS. DNA synthesis was also inhibited by aphidicolin when assayed by incorporation of [3H]thymidine into the DNA. The inhibitory effect of aphidicolin on the growth of E. histolytica was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 48 hr had little effect on the viability. Synchronous growth was observed in the recovery phase after removal of aphidicolin.",

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AU - Makioka, Asao

AU - Ohtomo, Hiroshi

AU - Kobayashi, Seiki

AU - Takeuchi, Tsutomu

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AB - We have previously demonstrated that DNA polymerase activity of Entamoeba histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative DNA polymerases. The present study was aimed to evaluate the effect of aphidicolin on growth and DNA synthesis by this parasite. Aphidicolin blocked the growth of axenic E. histolytica strain HM-1:IMSS. DNA synthesis was also inhibited by aphidicolin when assayed by incorporation of [3H]thymidine into the DNA. The inhibitory effect of aphidicolin on the growth of E. histolytica was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 48 hr had little effect on the viability. Synchronous growth was observed in the recovery phase after removal of aphidicolin.

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