Effects of bistheonellide A, an actin-polymerization inhibitor, on Chinese hamster V79 cells and on IL-8 production in PMA-stimulated HL-60 cells

Taiko Oda, Jinzhong Xu, Ayako Fujita, Masataka Mochizuki, Michio Namikoshi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bioassay-guided isolation from the ethanol extract of a marine sponge Theonella sp. collected in Palau yielded bistheonellide A, which strongly inhibited the colony formation of Chinese hamster V79 cells (EC 50 = 6.8 nM). Bistheonellide A is an actinpolymerization inhibitor and was suggested to control cytokine production. Therefore, we attempted to detect an effect of this compound on IL-8 production in PMA-stimulated HL-60 cells. Interestingly, bistheonellide A did not modulate the production of IL-8 under cytotoxic concentrations as determined by LDH analysis. Although the correlation between the inhibition of microtubule assembly and the stimulation of IL-8 production has been observed for several compounds, the polymerization of actin was not related to an IL-8 production in the case of bistheonellide A. It will be suggested that the actin polymerization is not involved in the IL-8 production system.

Original languageEnglish
Pages (from-to)22-27
Number of pages6
JournalMarine Drugs
Volume4
Issue number1
DOIs
Publication statusPublished - 2006 Mar

Keywords

  • Actin-polymerization inhibitor
  • Bistheonellide A
  • HL-60 cell
  • IL-8
  • V79 cell

ASJC Scopus subject areas

  • Drug Discovery

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