Effects of Carbon Black Nanoparticles on Elastase-Induced Emphysematous Lung Injury in Mice

Ken ichiro Inoue, Rie Yanagisawa, Eiko Koike, Ryosuke Nakamura, Takamichi Ichinose, Sadatomo Tasaka, Masako Kiyono, Hirohisa Takano

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Although adverse health effects of particulate matter with a diameter of <100nm (nanoparticles) have been proposed, biological evidence supporting their promotion of the inflammatory lung response in vivo is limited. This study investigated the impact of pulmonary exposure to carbon black nanoparticles (CBNP) on emphysematous lung injury induced by porcine pancreatic elastase (PPE) in mice. Vehicle, two sizes (14 and 56nm) of CBNP (50μg/body: 4mg/kg), PPE (0.03U/body: 1U/kg) or PPE+CBNP was administered intratracheally; thereafter, parameters of inflammatory lung changes were evaluated at several time-points. CBNP of 14nm significantly induced acute lung inflammation in non-elicited subjects and aggravated PPE-elicited airway neutrophilic inflammation at an early stage (day 1), which was concomitant with the enhanced lung expression of pro-inflammatory cytokines such as interleukin-1β and chemokine such as keratinocyte-derived chemoattractant. Further, 14-nm CBNP exaggerated emphysematous lung structural changes at a delayed stage (day 14). On the other hand, 56-nm CBNP induced lung inflammation but did not influence PPE-elicited pathophysiology in the lung. Taken together, CBNP at an optimal size and dose can exacerbate PPE-induced pulmonary inflammation and emphysema. This enhancement may be mediated, at least partly, via the increased local expression of pro-inflammatory molecules.

Original languageEnglish
Pages (from-to)234-240
Number of pages7
JournalBasic and Clinical Pharmacology and Toxicology
Volume108
Issue number4
DOIs
Publication statusPublished - 2011 Apr

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Soot
Pancreatic Elastase
Lung Injury
Nanoparticles
Swine
Lung
Pneumonia
Pulmonary Emphysema
Particulate Matter
Chemotactic Factors
Interleukin-1
Keratinocytes
Chemokines
Health
Cytokines
Inflammation
Molecules

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Inoue, K. I., Yanagisawa, R., Koike, E., Nakamura, R., Ichinose, T., Tasaka, S., ... Takano, H. (2011). Effects of Carbon Black Nanoparticles on Elastase-Induced Emphysematous Lung Injury in Mice. Basic and Clinical Pharmacology and Toxicology, 108(4), 234-240. https://doi.org/10.1111/j.1742-7843.2010.00638.x

Effects of Carbon Black Nanoparticles on Elastase-Induced Emphysematous Lung Injury in Mice. / Inoue, Ken ichiro; Yanagisawa, Rie; Koike, Eiko; Nakamura, Ryosuke; Ichinose, Takamichi; Tasaka, Sadatomo; Kiyono, Masako; Takano, Hirohisa.

In: Basic and Clinical Pharmacology and Toxicology, Vol. 108, No. 4, 04.2011, p. 234-240.

Research output: Contribution to journalArticle

Inoue, KI, Yanagisawa, R, Koike, E, Nakamura, R, Ichinose, T, Tasaka, S, Kiyono, M & Takano, H 2011, 'Effects of Carbon Black Nanoparticles on Elastase-Induced Emphysematous Lung Injury in Mice', Basic and Clinical Pharmacology and Toxicology, vol. 108, no. 4, pp. 234-240. https://doi.org/10.1111/j.1742-7843.2010.00638.x
Inoue, Ken ichiro ; Yanagisawa, Rie ; Koike, Eiko ; Nakamura, Ryosuke ; Ichinose, Takamichi ; Tasaka, Sadatomo ; Kiyono, Masako ; Takano, Hirohisa. / Effects of Carbon Black Nanoparticles on Elastase-Induced Emphysematous Lung Injury in Mice. In: Basic and Clinical Pharmacology and Toxicology. 2011 ; Vol. 108, No. 4. pp. 234-240.
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