Effects of Coffee on Estrogen Sulfation in Human Colon Carcinoma Caco-2 Cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Recent epidemiological studies have shown that moderate coffee consumption is associated with a lower risk of certain types of cancers, particularly colon cancer in postmenopausal women. To elucidate the molecular basis of the preventive action of coffee toward colon cancer, we focused on the effects of coffee on the metabolism of estrogens by sulfation, a reaction that inactivates estrogens. Estrogens are sulfonated by estrogen sulfotransferase (SULT1E1), and the sulfonated estrogens are reversely hydrolyzed by steroid sulfatase (STS). Sulfonated estrogens are pumped out of the cells by an ABC transporter, breast cancer resistance protein (BCRP). Intracellular concentrations of active estrogens may be determined by the balance of these activities. In human colon cancer Caco-2 cells, coffee decreases the expression of SULT1E1 and STS, whereas it increases BCRP expression. Activities of these three proteins change according to the expression level of each gene. Active constituents of coffee extract can be generated during the roasting process of coffee beans at different times. Modulation of the expression of these genes is likely to be mediated via activation of transcription factors such as NFκB (. BCRP) and stimulating protein 1 (. SULT1E1). These results suggest that coffee consumption may modulate the inactivation of estrogens by sulfation in human colon cells.

Original languageEnglish
Title of host publicationCoffee in Health and Disease Prevention
PublisherElsevier Inc.
Pages545-552
Number of pages8
ISBN (Print)9780124167162, 9780124095175
DOIs
Publication statusPublished - 2014 Nov 3

Fingerprint

Caco-2 Cells
Coffee
Estrogens
Colon
Carcinoma
Colonic Neoplasms
Steryl-Sulfatase
Breast Neoplasms
Proteins
ATP-Binding Cassette Transporters
Epidemiologic Studies
Transcription Factors
Gene Expression
Genes

Keywords

  • Breast cancer resistance protein
  • Caco-2
  • Coffee
  • Estrogen
  • Roasting
  • Steroid sulfatase
  • Sulfotransferase

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effects of Coffee on Estrogen Sulfation in Human Colon Carcinoma Caco-2 Cells. / Tamura, Hiroomi.

Coffee in Health and Disease Prevention. Elsevier Inc., 2014. p. 545-552.

Research output: Chapter in Book/Report/Conference proceedingChapter

Tamura, Hiroomi. / Effects of Coffee on Estrogen Sulfation in Human Colon Carcinoma Caco-2 Cells. Coffee in Health and Disease Prevention. Elsevier Inc., 2014. pp. 545-552
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