Effects of Cu, Zn-superoxide dismutase delivered by genetically modified skin fibroblasts on cold-induced skin edema in rats

Kohshi Nishiguchi, Fusao Komada, Yusuke Tanigawara, Yumiko Sakai, Seigo Iwakawa, Katsuhiko Okumura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effects of Cu, Zn-superoxide disumutase (SOD) delivered by genetically modified skin fibroblasts on cold-induced skin edema were studied in rats. Cold-induced skin edema, was induced on the dorsal skin following transplantation of ILSOD cells, genetically modified skin fibroblasts which release secretable SOD protein into the extracellular space. The degree of skin edema induced by cold injury was estimated by measuring the amounts of Evans' blue (EB) leaking into the injured skin following intravenously administration. The amounts of EB leakage were significantly reduced by transplantation of ILSOD cells relative to that observed following transplantation of host cells as a control. The degrees and durability of these effects of ILSOD cells were dependent on the number of cells transplanted. Also, the increases of lipid peroxidation following cold injury were significantly reduced by transplantation of ILSOD cells but not of host cells. These findings suggested that transplantation of ILSOD cells was a suitable delivery system for obtaining efficient and continuous effects of SOD. This strategy using genetically modified skin fibroblasts may also be useful as a drug delivery system for other therapeutic proteins.

Original languageEnglish
Pages (from-to)1379-1381
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume21
Issue number12
DOIs
Publication statusPublished - 1998 Jan 1

Keywords

  • Cell transplantation
  • Cold injury
  • Gene therapy
  • Genetically modified fibroblast
  • In vivo
  • SOD

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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