Effects of Cyclic Adenosine 3′,5′-Monophosphate on Chondrocyte Terminal Differentiation and Cartilage-Matrix Calcification

Akitoshi Jikko, Hiroshi Murakami, Weiqun Yan, Kazuhisa Nakashima, Yoshie Ohya, Hisashi Satakeda, Mitsuhide Noshiro, Takeshi Kawamoto, Shigeo Nakamura, Yasunori Okada, Fujio Suzuki, Yukio Kato

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We examined the effects of cyclic AMP on terminal differentiation and calcification in rabbit growth plate chondrocyte cultures. Dibutyryl cAMP (dbcAMP), as well as 8-bromo-cAMP abolished the increases in chondrocyte size, alkaline phosphatase activity, type X collagen synthesis, 1α,25-dihydroxyvitamin D3 receptor synthesis, the incorporation of 45Ca into insoluble material, and the calcium content. All of these occurred in parallel untreated cultures during the hypertrophic (terminal) stage. The inhibition of alkaline phosphatase by dbcAMP was detectable after 24 h, and this effect was reversible. dbcAMP and 8-bromo-cyclic AMP inhibited alkaline phosphatase induction and calcification at low concentrations (3-5 μM), whereas 10-to 30-fold higher concentrations were required to stimulate proteoglycan synthesis. These findings suggest that cAMP plays a crucial role in suppressing terminal differentiation of chondrocytes and cartilage-matrix calcification.

Original languageEnglish
Pages (from-to)122-128
Number of pages7
JournalEndocrinology
Volume137
Issue number1
Publication statusPublished - 1996

Fingerprint

Chondrocytes
Adenosine
Cartilage
Alkaline Phosphatase
8-Bromo Cyclic Adenosine Monophosphate
Collagen Type X
Calcitriol Receptors
Growth Plate
Proteoglycans
Cyclic AMP
Rabbits
Calcium

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Jikko, A., Murakami, H., Yan, W., Nakashima, K., Ohya, Y., Satakeda, H., ... Kato, Y. (1996). Effects of Cyclic Adenosine 3′,5′-Monophosphate on Chondrocyte Terminal Differentiation and Cartilage-Matrix Calcification. Endocrinology, 137(1), 122-128.

Effects of Cyclic Adenosine 3′,5′-Monophosphate on Chondrocyte Terminal Differentiation and Cartilage-Matrix Calcification. / Jikko, Akitoshi; Murakami, Hiroshi; Yan, Weiqun; Nakashima, Kazuhisa; Ohya, Yoshie; Satakeda, Hisashi; Noshiro, Mitsuhide; Kawamoto, Takeshi; Nakamura, Shigeo; Okada, Yasunori; Suzuki, Fujio; Kato, Yukio.

In: Endocrinology, Vol. 137, No. 1, 1996, p. 122-128.

Research output: Contribution to journalArticle

Jikko, A, Murakami, H, Yan, W, Nakashima, K, Ohya, Y, Satakeda, H, Noshiro, M, Kawamoto, T, Nakamura, S, Okada, Y, Suzuki, F & Kato, Y 1996, 'Effects of Cyclic Adenosine 3′,5′-Monophosphate on Chondrocyte Terminal Differentiation and Cartilage-Matrix Calcification', Endocrinology, vol. 137, no. 1, pp. 122-128.
Jikko, Akitoshi ; Murakami, Hiroshi ; Yan, Weiqun ; Nakashima, Kazuhisa ; Ohya, Yoshie ; Satakeda, Hisashi ; Noshiro, Mitsuhide ; Kawamoto, Takeshi ; Nakamura, Shigeo ; Okada, Yasunori ; Suzuki, Fujio ; Kato, Yukio. / Effects of Cyclic Adenosine 3′,5′-Monophosphate on Chondrocyte Terminal Differentiation and Cartilage-Matrix Calcification. In: Endocrinology. 1996 ; Vol. 137, No. 1. pp. 122-128.
@article{759d5f119b7849d4bbf00aacb80d42ed,
title = "Effects of Cyclic Adenosine 3′,5′-Monophosphate on Chondrocyte Terminal Differentiation and Cartilage-Matrix Calcification",
abstract = "We examined the effects of cyclic AMP on terminal differentiation and calcification in rabbit growth plate chondrocyte cultures. Dibutyryl cAMP (dbcAMP), as well as 8-bromo-cAMP abolished the increases in chondrocyte size, alkaline phosphatase activity, type X collagen synthesis, 1α,25-dihydroxyvitamin D3 receptor synthesis, the incorporation of 45Ca into insoluble material, and the calcium content. All of these occurred in parallel untreated cultures during the hypertrophic (terminal) stage. The inhibition of alkaline phosphatase by dbcAMP was detectable after 24 h, and this effect was reversible. dbcAMP and 8-bromo-cyclic AMP inhibited alkaline phosphatase induction and calcification at low concentrations (3-5 μM), whereas 10-to 30-fold higher concentrations were required to stimulate proteoglycan synthesis. These findings suggest that cAMP plays a crucial role in suppressing terminal differentiation of chondrocytes and cartilage-matrix calcification.",
author = "Akitoshi Jikko and Hiroshi Murakami and Weiqun Yan and Kazuhisa Nakashima and Yoshie Ohya and Hisashi Satakeda and Mitsuhide Noshiro and Takeshi Kawamoto and Shigeo Nakamura and Yasunori Okada and Fujio Suzuki and Yukio Kato",
year = "1996",
language = "English",
volume = "137",
pages = "122--128",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "1",

}

TY - JOUR

T1 - Effects of Cyclic Adenosine 3′,5′-Monophosphate on Chondrocyte Terminal Differentiation and Cartilage-Matrix Calcification

AU - Jikko, Akitoshi

AU - Murakami, Hiroshi

AU - Yan, Weiqun

AU - Nakashima, Kazuhisa

AU - Ohya, Yoshie

AU - Satakeda, Hisashi

AU - Noshiro, Mitsuhide

AU - Kawamoto, Takeshi

AU - Nakamura, Shigeo

AU - Okada, Yasunori

AU - Suzuki, Fujio

AU - Kato, Yukio

PY - 1996

Y1 - 1996

N2 - We examined the effects of cyclic AMP on terminal differentiation and calcification in rabbit growth plate chondrocyte cultures. Dibutyryl cAMP (dbcAMP), as well as 8-bromo-cAMP abolished the increases in chondrocyte size, alkaline phosphatase activity, type X collagen synthesis, 1α,25-dihydroxyvitamin D3 receptor synthesis, the incorporation of 45Ca into insoluble material, and the calcium content. All of these occurred in parallel untreated cultures during the hypertrophic (terminal) stage. The inhibition of alkaline phosphatase by dbcAMP was detectable after 24 h, and this effect was reversible. dbcAMP and 8-bromo-cyclic AMP inhibited alkaline phosphatase induction and calcification at low concentrations (3-5 μM), whereas 10-to 30-fold higher concentrations were required to stimulate proteoglycan synthesis. These findings suggest that cAMP plays a crucial role in suppressing terminal differentiation of chondrocytes and cartilage-matrix calcification.

AB - We examined the effects of cyclic AMP on terminal differentiation and calcification in rabbit growth plate chondrocyte cultures. Dibutyryl cAMP (dbcAMP), as well as 8-bromo-cAMP abolished the increases in chondrocyte size, alkaline phosphatase activity, type X collagen synthesis, 1α,25-dihydroxyvitamin D3 receptor synthesis, the incorporation of 45Ca into insoluble material, and the calcium content. All of these occurred in parallel untreated cultures during the hypertrophic (terminal) stage. The inhibition of alkaline phosphatase by dbcAMP was detectable after 24 h, and this effect was reversible. dbcAMP and 8-bromo-cyclic AMP inhibited alkaline phosphatase induction and calcification at low concentrations (3-5 μM), whereas 10-to 30-fold higher concentrations were required to stimulate proteoglycan synthesis. These findings suggest that cAMP plays a crucial role in suppressing terminal differentiation of chondrocytes and cartilage-matrix calcification.

UR - http://www.scopus.com/inward/record.url?scp=0030026179&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030026179&partnerID=8YFLogxK

M3 - Article

C2 - 8536602

AN - SCOPUS:0030026179

VL - 137

SP - 122

EP - 128

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 1

ER -