Effects of Cyclic Adenosine 3′,5′-Monophosphate on Chondrocyte Terminal Differentiation and Cartilage-Matrix Calcification

Akitoshi Jikko, Hiroshi Murakami, Weiqun Yan, Kazuhisa Nakashima, Yoshie Ohya, Hisashi Satakeda, Mitsuhide Noshiro, Takeshi Kawamoto, Shigeo Nakamura, Yasunori Okada, Fujio Suzuki, Yukio Kato

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29 Citations (Scopus)

Abstract

We examined the effects of cyclic AMP on terminal differentiation and calcification in rabbit growth plate chondrocyte cultures. Dibutyryl cAMP (dbcAMP), as well as 8-bromo-cAMP abolished the increases in chondrocyte size, alkaline phosphatase activity, type X collagen synthesis, 1α,25-dihydroxyvitamin D3 receptor synthesis, the incorporation of 45Ca into insoluble material, and the calcium content. All of these occurred in parallel untreated cultures during the hypertrophic (terminal) stage. The inhibition of alkaline phosphatase by dbcAMP was detectable after 24 h, and this effect was reversible. dbcAMP and 8-bromo-cyclic AMP inhibited alkaline phosphatase induction and calcification at low concentrations (3-5 μM), whereas 10-to 30-fold higher concentrations were required to stimulate proteoglycan synthesis. These findings suggest that cAMP plays a crucial role in suppressing terminal differentiation of chondrocytes and cartilage-matrix calcification.

Original languageEnglish
Pages (from-to)122-128
Number of pages7
JournalEndocrinology
Volume137
Issue number1
DOIs
Publication statusPublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology

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    Jikko, A., Murakami, H., Yan, W., Nakashima, K., Ohya, Y., Satakeda, H., Noshiro, M., Kawamoto, T., Nakamura, S., Okada, Y., Suzuki, F., & Kato, Y. (1996). Effects of Cyclic Adenosine 3′,5′-Monophosphate on Chondrocyte Terminal Differentiation and Cartilage-Matrix Calcification. Endocrinology, 137(1), 122-128. https://doi.org/10.1210/endo.137.1.8536602