Effects of gonadotropin-releasing hormone agonist (GnRHa) and ovariectomy on leukocyte subpopulations in rats with autotransplanted endometrium

Hidehiko Matsubayashi, Ken Ichi Iwasaki, Tetsuo Maruyama, Nobuaki Ozawa, Taiko Nemoto, Shinya Iwasaki, Wen Shu Sun, Kazumi Nakazawa, Shiro Nozawa, Tsunehisa Makino

Research output: Contribution to journalArticle

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Abstract

PROBLEM: To determine the effect of estrogen deficiency on populations of immune cells in rats with autotransplanted endometrium. METHOD OF STUDY: Autologous endometrial segments were implanted in the peritoneum of rats. Leukocyte subsets in the peripheral blood (PB) and peritoneal fluid (PF) of autotransplanted rats treated with gonadotropin-releasing hormone agonist (GnRHa) or ovariectomy were analyzed by flow cytometry. RESULTS: Endometrial growth was suppressed in association with increases in natural killer (NK) cells and macrophages in rats treated surgically or medically after endometrial implantation compared with autotransplanted-only rats. Normal rats treated with GnRHa showed no significant alterations in PB and PF leukocyte subsets, even in NK cells and macrophages, compared with untreated normal rats. CONCLUSIONS: Data suggest that GnRHa itself did not have an immunomodulating effect when rats were treated for 3 weeks, but that a hypoestrogenic state reduced the size of the ectopic endometrium in association with increases in NK cells and macrophages. Our findings support the hypothesis that ectopic endometrial cells may release immunosuppressive factors.

Original languageEnglish
Pages (from-to)40-48
Number of pages9
JournalAmerican Journal of Reproductive Immunology
Volume36
Issue number1
Publication statusPublished - 1996 Jul

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Ovariectomy
Endometrium
Gonadotropin-Releasing Hormone
Leukocytes
Natural Killer Cells
Ascitic Fluid
Macrophages
Peritoneum
Immunosuppressive Agents
Flow Cytometry
Estrogens
Growth
Population

Keywords

  • Animal model
  • Endometriosis
  • Flow cytometry
  • Macrophage
  • NK cell

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynaecology

Cite this

Effects of gonadotropin-releasing hormone agonist (GnRHa) and ovariectomy on leukocyte subpopulations in rats with autotransplanted endometrium. / Matsubayashi, Hidehiko; Iwasaki, Ken Ichi; Maruyama, Tetsuo; Ozawa, Nobuaki; Nemoto, Taiko; Iwasaki, Shinya; Sun, Wen Shu; Nakazawa, Kazumi; Nozawa, Shiro; Makino, Tsunehisa.

In: American Journal of Reproductive Immunology, Vol. 36, No. 1, 07.1996, p. 40-48.

Research output: Contribution to journalArticle

Matsubayashi, H, Iwasaki, KI, Maruyama, T, Ozawa, N, Nemoto, T, Iwasaki, S, Sun, WS, Nakazawa, K, Nozawa, S & Makino, T 1996, 'Effects of gonadotropin-releasing hormone agonist (GnRHa) and ovariectomy on leukocyte subpopulations in rats with autotransplanted endometrium', American Journal of Reproductive Immunology, vol. 36, no. 1, pp. 40-48.
Matsubayashi, Hidehiko ; Iwasaki, Ken Ichi ; Maruyama, Tetsuo ; Ozawa, Nobuaki ; Nemoto, Taiko ; Iwasaki, Shinya ; Sun, Wen Shu ; Nakazawa, Kazumi ; Nozawa, Shiro ; Makino, Tsunehisa. / Effects of gonadotropin-releasing hormone agonist (GnRHa) and ovariectomy on leukocyte subpopulations in rats with autotransplanted endometrium. In: American Journal of Reproductive Immunology. 1996 ; Vol. 36, No. 1. pp. 40-48.
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