Effects of hexasulfobutylated C60 on the gastric circular muscle of guinea pig

S. S. Huang, L. H. Chih, C. H. Lin, L. Y. Chiang, Tadahiko Mashino, M. Mochizuki, K. Okuda, T. Hirota, M. C. Tsai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effects of hexasulfobutylated (FC4S), the fullerene derivative on the contraction of smooth muscle were tested pharmacologically on the circular muscle of stomach of guinea pigs. The effects of monomalonic acid C60 (MMA C60) on the same preparations were compared. The effects of those compounds on the taenia coli, portal vein and vas deferens of guinea pigs were also tested. The FC4S did, while MMA C60 did not elicit contracture of the circular muscle of stomach and taenia coli. Both FC4S and MMA C60 did not elicit contraction on the portal vein and on the vas deferens of the guinea pig. Prazosin (0.5 μM) or propranolol (0.5 μM) did not alter the FC4S elicit contracture of the circular muscle of stomach. However, atropine (0.01, 0.1 and 1 μM), tetrodotoxin (0.1 μM) or low calcium medium decreased reversibly the FC4S elicited contracture of the circular muscle of stomach. The effect of FC4S on the contracture of the gastric muscles was also tested using various muscarinic receptor subtype antagonists. 4-DAMP (1 μM), muscarinic M3 receptor antagonist, and tropicamide (1 μM), muscarinic M4 receptor antagonist, did not alter the contracture elicited by FC4S. Pirenzepine (0.1 μM), muscarinic M1 receptor antagonist, and methoctramine (0.25 μM), muscarinic M2 receptor antagonist, significantly decreased the FC4S elicited contracture of the circular muscle. Atropine (1 μM) or tetrodotoxin (0.1 μM) completely blocked the FC4S elicited contracture of the circular muscle of stomach. It is concluded that FC4S elicited contracture of the circular muscle of stomach. The effect may be due to FC4S acts on the cholinergic cells existed in the gastric muscle and indirectly activating the tetrodotoxin dependent releasing of the transmitters from the cells, then, activating the muscarinice M1, M2 receptors in the muscle eliciting the contractures.

Original languageEnglish
Pages (from-to)375-395
Number of pages21
JournalFullerene Science and Technology
Volume9
Issue number3
DOIs
Publication statusPublished - 2001 Aug
Externally publishedYes

Fingerprint

guinea pigs
muscles
Muscle
stomach
atropine
Tetrodotoxin
veins
acids
contraction
Atropine
Acids
cholinergics
Muscarinic M4 Receptors
Tropicamide
Muscarinic M3 Receptors
smooth muscle
Muscarinic M2 Receptors
Muscarinic M1 Receptors
Fullerenes
Pirenzepine

Keywords

  • Contraction
  • Fullerene derivative
  • Gastric circular muscle
  • Hexasulfobutylated (FCS)
  • Monomalonic acid C (MMA C)
  • Receptor

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Huang, S. S., Chih, L. H., Lin, C. H., Chiang, L. Y., Mashino, T., Mochizuki, M., ... Tsai, M. C. (2001). Effects of hexasulfobutylated C60 on the gastric circular muscle of guinea pig. Fullerene Science and Technology, 9(3), 375-395. https://doi.org/10.1081/FST-100104501

Effects of hexasulfobutylated C60 on the gastric circular muscle of guinea pig. / Huang, S. S.; Chih, L. H.; Lin, C. H.; Chiang, L. Y.; Mashino, Tadahiko; Mochizuki, M.; Okuda, K.; Hirota, T.; Tsai, M. C.

In: Fullerene Science and Technology, Vol. 9, No. 3, 08.2001, p. 375-395.

Research output: Contribution to journalArticle

Huang, SS, Chih, LH, Lin, CH, Chiang, LY, Mashino, T, Mochizuki, M, Okuda, K, Hirota, T & Tsai, MC 2001, 'Effects of hexasulfobutylated C60 on the gastric circular muscle of guinea pig', Fullerene Science and Technology, vol. 9, no. 3, pp. 375-395. https://doi.org/10.1081/FST-100104501
Huang, S. S. ; Chih, L. H. ; Lin, C. H. ; Chiang, L. Y. ; Mashino, Tadahiko ; Mochizuki, M. ; Okuda, K. ; Hirota, T. ; Tsai, M. C. / Effects of hexasulfobutylated C60 on the gastric circular muscle of guinea pig. In: Fullerene Science and Technology. 2001 ; Vol. 9, No. 3. pp. 375-395.
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N2 - The effects of hexasulfobutylated (FC4S), the fullerene derivative on the contraction of smooth muscle were tested pharmacologically on the circular muscle of stomach of guinea pigs. The effects of monomalonic acid C60 (MMA C60) on the same preparations were compared. The effects of those compounds on the taenia coli, portal vein and vas deferens of guinea pigs were also tested. The FC4S did, while MMA C60 did not elicit contracture of the circular muscle of stomach and taenia coli. Both FC4S and MMA C60 did not elicit contraction on the portal vein and on the vas deferens of the guinea pig. Prazosin (0.5 μM) or propranolol (0.5 μM) did not alter the FC4S elicit contracture of the circular muscle of stomach. However, atropine (0.01, 0.1 and 1 μM), tetrodotoxin (0.1 μM) or low calcium medium decreased reversibly the FC4S elicited contracture of the circular muscle of stomach. The effect of FC4S on the contracture of the gastric muscles was also tested using various muscarinic receptor subtype antagonists. 4-DAMP (1 μM), muscarinic M3 receptor antagonist, and tropicamide (1 μM), muscarinic M4 receptor antagonist, did not alter the contracture elicited by FC4S. Pirenzepine (0.1 μM), muscarinic M1 receptor antagonist, and methoctramine (0.25 μM), muscarinic M2 receptor antagonist, significantly decreased the FC4S elicited contracture of the circular muscle. Atropine (1 μM) or tetrodotoxin (0.1 μM) completely blocked the FC4S elicited contracture of the circular muscle of stomach. It is concluded that FC4S elicited contracture of the circular muscle of stomach. The effect may be due to FC4S acts on the cholinergic cells existed in the gastric muscle and indirectly activating the tetrodotoxin dependent releasing of the transmitters from the cells, then, activating the muscarinice M1, M2 receptors in the muscle eliciting the contractures.

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