Effects of in vivo and in vitro alkali treatment on intracellular pH regulation of OMCDis cells

Matsuhiko Hayashi, Masahiro Iyori, Yasuyoshi Yamaji, Takao Saruta

Research output: Contribution to journalArticle

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Abstract

To examine functional changes of the transporters in the inner stripe of the outer medullary collecting ducts (OMCDis) by the peritubular acid-base status, in vitro microperfusion using the acetoxymethyl ester of 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein was performed. Cell alkalinization systems were assessed by the recovery rate (dpHi/dt) of intracellular pH (pHi) after intracellular acid loading by NH4+-NH3 prepulse with bath amiloride. In alkali-loaded rabbits (0.15 M NaHCO3 drinking for 14 days), dpHi/dt showed a significant decrease (1.80 ± 0.29 pH units/s X 103) compared with either control (3.30 ± 0.59) or acid-loaded rabbits (0.15 M NH4Cl drinking for 14 days, 3.05 ± 0.46). The difference of dpHi/dt between control and alkali-loaded rabbits was eliminated by lumen N-ethylmaleimide (NEM), suggesting that H+ pump activity was decreased. The effect of in vitro alkali treatment (50 mM HCO3-, pH 7.7) for 3-4 h was also examined. This incubation significantly decreased the dpHi/dt (1.83 ± 0.35) compared with the time control experiments (3.18 ± 0.28), whereas no significant difference was seen in the presence of lumen NEM. Anion exchanger activity, as determined from the pHi changes after Cl- addition to the bath, showed no significant change with in vivo or in vitro alkali treatment. The results indicate that cell function of the OMCDis is regulated in response to the peritubular acid-base environment via changes in the H+-adenosinetriphosphatase.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume265
Issue number5 34-5
Publication statusPublished - 1993 Nov

Fingerprint

Alkalies
Ethylmaleimide
Acids
Rabbits
Baths
Drinking
Proton Pumps
Amiloride
Therapeutics
Anions
Adenosine Triphosphatases
Esters
In Vitro Techniques

Keywords

  • Anion exchanger
  • Inner stripe of outer medullary collecting ducts
  • Microperfusion
  • Proton-translocating adenosinetriphosphatase

ASJC Scopus subject areas

  • Physiology

Cite this

Effects of in vivo and in vitro alkali treatment on intracellular pH regulation of OMCDis cells. / Hayashi, Matsuhiko; Iyori, Masahiro; Yamaji, Yasuyoshi; Saruta, Takao.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 265, No. 5 34-5, 11.1993.

Research output: Contribution to journalArticle

Hayashi, Matsuhiko ; Iyori, Masahiro ; Yamaji, Yasuyoshi ; Saruta, Takao. / Effects of in vivo and in vitro alkali treatment on intracellular pH regulation of OMCDis cells. In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1993 ; Vol. 265, No. 5 34-5.
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