Effects of insulin-like growth factor-1 on B-cell precursor acute lymphoblastic leukemia

Hiroyuki Yamada, Kazutoshi Iijima, Osamu Tomita, Tomoko Taguchi, Masashi Miharu, Kenichiro Kobayashi, Hajime Okita, Masahiro Saito, Toshiaki Shimizu, Nobutaka Kiyokawa

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Insulin-like growth factor-1 (IGF-1) is known to be a major growth factor with effects on various cell types, including hematopoietic cells, as well as neoplasms, and is regulated by IGF-binding proteins (IGFBPs). In this study, we investigated the effects of IGF-1 on B-cell precursor acute lymphoblastic leukemia (BCP-ALL) cells. When the expression of IGF-1R in clinical samples of BCP-ALL was examined, five of thirty-two cases showed IGF-1R expression, whereas IGF-1R was expressed in most BCP-ALL cell lines. We observed that IGF-1 enhanced the proliferation of BCP-ALL cell lines that can be partially inhibited by IGFBP-1, -3, and -4, but not other IGFBPs. IGF-1 also partially inhibited dexamethasone-induced apoptosis, but not apoptosis mediated by VP-16 and irradiation. Interestingly, the proliferative effect of IGF-1 was partially blocked by inhibitors of MAPK and AKT, whereas the inhibition of dexamethasone-induced apoptosis was completely blocked by both inhibitors. Our data indicate that IGF-1 is involved in cell proliferation and apoptosis regulation in BCP-ALL cells. Since some BCP-ALL cases express IGF-1R, it appears to be a plausible target for prognostic evaluation and may represent a new therapeutic strategy.

Original languageEnglish
Pages (from-to)73-82
Number of pages10
JournalInternational Journal of Hematology
Volume97
Issue number1
DOIs
Publication statusPublished - 2013 Jan
Externally publishedYes

Fingerprint

B-Lymphoid Precursor Cells
Somatomedins
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Apoptosis
Insulin-Like Growth Factor Binding Proteins
Dexamethasone
Insulin-Like Growth Factor Binding Protein 1
Cell Line
Insulin-Like Growth Factor Binding Protein 3
Etoposide
Intercellular Signaling Peptides and Proteins
Cell Proliferation
Neoplasms

Keywords

  • Apoptosis
  • B-cell precursor ALL
  • Cell growth
  • IGF-1
  • IGFBP

ASJC Scopus subject areas

  • Hematology

Cite this

Yamada, H., Iijima, K., Tomita, O., Taguchi, T., Miharu, M., Kobayashi, K., ... Kiyokawa, N. (2013). Effects of insulin-like growth factor-1 on B-cell precursor acute lymphoblastic leukemia. International Journal of Hematology, 97(1), 73-82. https://doi.org/10.1007/s12185-012-1234-3

Effects of insulin-like growth factor-1 on B-cell precursor acute lymphoblastic leukemia. / Yamada, Hiroyuki; Iijima, Kazutoshi; Tomita, Osamu; Taguchi, Tomoko; Miharu, Masashi; Kobayashi, Kenichiro; Okita, Hajime; Saito, Masahiro; Shimizu, Toshiaki; Kiyokawa, Nobutaka.

In: International Journal of Hematology, Vol. 97, No. 1, 01.2013, p. 73-82.

Research output: Contribution to journalArticle

Yamada, H, Iijima, K, Tomita, O, Taguchi, T, Miharu, M, Kobayashi, K, Okita, H, Saito, M, Shimizu, T & Kiyokawa, N 2013, 'Effects of insulin-like growth factor-1 on B-cell precursor acute lymphoblastic leukemia', International Journal of Hematology, vol. 97, no. 1, pp. 73-82. https://doi.org/10.1007/s12185-012-1234-3
Yamada, Hiroyuki ; Iijima, Kazutoshi ; Tomita, Osamu ; Taguchi, Tomoko ; Miharu, Masashi ; Kobayashi, Kenichiro ; Okita, Hajime ; Saito, Masahiro ; Shimizu, Toshiaki ; Kiyokawa, Nobutaka. / Effects of insulin-like growth factor-1 on B-cell precursor acute lymphoblastic leukemia. In: International Journal of Hematology. 2013 ; Vol. 97, No. 1. pp. 73-82.
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