Effects of intravenously administered β-human atrial natriuretic polypeptide in humans

Hiroshi Itoh, Kazuwa Nakao, Masashi Mukoyama, Shozo Shiono, Narito Morii, Akira Sugawara, Takayuki Yamada, Yoshihiko Saito, Hiroshi Arai, Klminori Hosoda, Yoshikazu Kambayashi, Ken Inouye, Hlroo Imura

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Abstract

β-Human atrial natriuretic polypeptide (β-hANP) is an antiparallel dimer of α-human ANP (a-hANP) that was isolated from human atria. Using synthetic β-hANP and a radioimmunoassay for α-hANP that also detects β-hANP, we have previously demonstrated that β-hANP is converted into α-hANP in human plasma in vitro. In the present study, we compared the effects of intravenous administration of β-hANP (100 μg) to five normal human volunteers with those of an equimolar administration of α-hANP (50 μg) to the same subjects, and we also investigated the possible mechanisms of actions of β-hANP. Although the administration of α-hANP caused a significant decrease in blood pressure with a reactional increase of heart rate, β-hANP elicited minimal change of blood pressure. In contrast, β-hANP exerted more potent and longer lasting diuretic and natriuretic activities than did α-hANP. Net changes in urine volume and sodium excretion induced by β-hANP (579 ± 65 ml, 56.0 ± 9.9 mEq) were significantly greater than those elicited by α-hANP (396 ± 50 ml, 34.7 ± 4.9 mEq; p < 0.05, respectively). The administration ofβ-hANP revealed a longer retention of the ANP-like immunoreactivity level in plasma, compared with that of α-hANP. High performance gel permeation chromatography coupled with the radioimmunoassay revealed that β-hANP (Afr=6000) was also converted into α-hANP (Mr=3000) in human plasma in vivo. The demonstrated conversion of β-hANP into α-hANP could be relevant to the observed effects of β-hANP in humans.

Original languageEnglish
Pages (from-to)697-702
Number of pages6
JournalHypertension
Volume11
Issue number6
Publication statusPublished - 1988 Jun

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Keywords

  • Atrial natriuretic factor
  • Blood pressure
  • Diuresis
  • Glomerular filtration rate
  • Heart rate
  • Natriuresis

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Itoh, H., Nakao, K., Mukoyama, M., Shiono, S., Morii, N., Sugawara, A., Yamada, T., Saito, Y., Arai, H., Hosoda, K., Kambayashi, Y., Inouye, K., & Imura, H. (1988). Effects of intravenously administered β-human atrial natriuretic polypeptide in humans. Hypertension, 11(6), 697-702.