Effects of laser parameters on propagation characteristics of laser-induced stress wave for gene transfer

Takahiro Ando, Shunichi Sato, Mitsuhiro Terakawa, Hiroshi Ashida, Minoru Obara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Laser-based gene delivery is attractive as a new method for topical gene therapy because of the high spatial controllability of laser energy. Previously, we demonstrated that an exogenous gene can be transferred to cells both in vitro and in vivo by applying nanosecond pulsed laser-induced stress waves (LISWs) or photomechanical waves (PMWs). In this study, we investigated effects of laser parameters on the propagation characteristics of LISWs in soft tissue phantoms and depth-dependent properties of gene transfection. Temporal pressure profiles of LISWs were measured with a hydrophone, showing that with a larger laser spot diameter, LISWs can be propagated more efficiently in phantoms with keeping flat wavefront. Phantoms with various thicknesses were placed on the rat dorsal skin that had been injected with plasmid DNA coding for reporter gene, and LISWs were applied from the top of the phantom. Efficient gene expression was observed in the rat skin that had interacted with LISWs propagating through a 15-mm-thick phantom. These results would be useful to determine appropriate laser parameters for gene delivery to deep-located tissue by transcutaneous application of LISWs.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7562
DOIs
Publication statusPublished - 2010
EventOptical Interactions with Tissues and Cells XXI - San Francisco, CA, United States
Duration: 2010 Jan 252010 Jan 27

Other

OtherOptical Interactions with Tissues and Cells XXI
CountryUnited States
CitySan Francisco, CA
Period10/1/2510/1/27

Fingerprint

Gene transfer
stress waves
genes
Lasers
propagation
Genes
lasers
rats
Rats
delivery
Skin
Tissue
gene therapy
Gene therapy
Hydrophones
plasmids
gene expression
hydrophones
controllability
Wavefronts

Keywords

  • Gene delivery
  • Laser-induced stress wave
  • Photomechanical wave
  • Propagation characteristics

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Ando, T., Sato, S., Terakawa, M., Ashida, H., & Obara, M. (2010). Effects of laser parameters on propagation characteristics of laser-induced stress wave for gene transfer. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7562). [75620Y] https://doi.org/10.1117/12.840815

Effects of laser parameters on propagation characteristics of laser-induced stress wave for gene transfer. / Ando, Takahiro; Sato, Shunichi; Terakawa, Mitsuhiro; Ashida, Hiroshi; Obara, Minoru.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7562 2010. 75620Y.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ando, T, Sato, S, Terakawa, M, Ashida, H & Obara, M 2010, Effects of laser parameters on propagation characteristics of laser-induced stress wave for gene transfer. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7562, 75620Y, Optical Interactions with Tissues and Cells XXI, San Francisco, CA, United States, 10/1/25. https://doi.org/10.1117/12.840815
Ando T, Sato S, Terakawa M, Ashida H, Obara M. Effects of laser parameters on propagation characteristics of laser-induced stress wave for gene transfer. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7562. 2010. 75620Y https://doi.org/10.1117/12.840815
Ando, Takahiro ; Sato, Shunichi ; Terakawa, Mitsuhiro ; Ashida, Hiroshi ; Obara, Minoru. / Effects of laser parameters on propagation characteristics of laser-induced stress wave for gene transfer. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7562 2010.
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