Effects of leptin receptor gene 3'-untranslated region polymorphism on metabolic profiles in young Japanese men.

Kanako Nishikai, Hiroshi Hirose, Tatsuya Ishii, Matsuhiko Hayashi, Ikuo Saito, Takao Saruta

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We investigated the effects of leptin receptor gene 3'-untranslated region (3'-UTR) polymorphism on clinical and metabolic parameters in 221 young Japanese men aged 21 to 28 years. The polymerase chain reaction-restriction fragment length polymorphism method was used to identify a pentanucleotide (CTTTA) insertion in 3'-UTR of the leptin receptor gene. Body mass index, blood pressure, plasma glucose, insulin, leptin, total cholesterol, high-density lipoprotein (HDL)-cholesterol, low-density lipoprotein (LDL)-cholesterol, triglycerides, free fatty acids, uric acid, apolipoprotein A-I (apoA-I), and cholesteryl-ester transfer protein levels were measured. There was only 1 homozygote and 38 heterozygotes for the 3'-UTR insertion allele among the 221 subjects. The insertion allele frequency was 0.090. Plasma HDL-cholesterol and apoA-I levels were significantly lower (p = 0.015 and p = 0.032 by Mann-Whitney U test, respectively) in homozygous or heterozygous carriers of the insertion allele than in subjects homozygous for the normal allele. There were no differences in other parameters measured. Furthermore, when the subjects were divided into three groups according to HDL-cholesterol level, the percentage of insertion allele-positive subjects was significantly lower in the highest HDL-cholesterol group (chi(2) = 8.42, p = 0.015). These findings suggest that serum HDL-cholesterol and apoA-I levels are influenced by the leptin receptor gene 3'-UTR polymorphism in young Japanese men.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalJournal of Atherosclerosis and Thrombosis
Volume11
Issue number2
Publication statusPublished - 2004

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Leptin Receptors
Metabolome
3' Untranslated Regions
Polymorphism
HDL Cholesterol
Genes
Apolipoprotein A-I
Alleles
Cholesterol Ester Transfer Proteins
Plasmas
Polymerase chain reaction
Cholesterol
Blood pressure
Homozygote
Heterozygote
Nonparametric Statistics
Leptin
Uric Acid
Nonesterified Fatty Acids
Gene Frequency

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Internal Medicine
  • Biochemistry, medical

Cite this

Effects of leptin receptor gene 3'-untranslated region polymorphism on metabolic profiles in young Japanese men. / Nishikai, Kanako; Hirose, Hiroshi; Ishii, Tatsuya; Hayashi, Matsuhiko; Saito, Ikuo; Saruta, Takao.

In: Journal of Atherosclerosis and Thrombosis, Vol. 11, No. 2, 2004, p. 73-78.

Research output: Contribution to journalArticle

Nishikai, Kanako ; Hirose, Hiroshi ; Ishii, Tatsuya ; Hayashi, Matsuhiko ; Saito, Ikuo ; Saruta, Takao. / Effects of leptin receptor gene 3'-untranslated region polymorphism on metabolic profiles in young Japanese men. In: Journal of Atherosclerosis and Thrombosis. 2004 ; Vol. 11, No. 2. pp. 73-78.
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