Effects of local administration of vascular endothelial growth factor on mechanical characteristics of the semitendinosus tendon graft after anterior cruciate ligament reconstruction in sheep

Toshikazu Yoshikawa, Harukazu Tohyama, Taro Katsura, Eiji Kondo, Yoshihisa Kotani, Hideo Matsumoto, Yoshiaki Toyama, Kazunori Yasuda

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Background: Vascular endothelial growth factor (VEGF) is a potent mediator of angiogenesis. Hypothesis: An application of VEGF may enhance angiogenesis in the grafted tendon in anterior cruciate ligament (ACL) reconstruction, and the application may affect mechanical characteristics of the ACL graft. Study Design: Controlled laboratory study. Methods: Eighteen sheep were divided into groups I and II. In group I, the harvested semitendinosus tendon was soaked in VEGF solution, and the right knee then underwent ACL reconstruction using this tendon. In group II, the right knee underwent identical procedures to those of group I except that the harvested tendon was soaked in phosphate-buffered saline. All animals were sacrificed 12 weeks after ACL reconstruction. Results: Histologic findings showed that newly formed vessels and infiltrative fibroblasts were more abundant in group I than in group II. The anterior-posterior translation of the knee during an anterior-posterior force of ±100 N was significantly larger in group I than in group II by 2.58 mm (95% confidence interval, -1.76 mm to 1.76 mm) (P = .002). The linear stiffness of the femur-graft-tibia complex in group I was significantly lower than that in group II by 41.5 N/mm (95% confidence interval, -32.2 N/mm to 32.2 N/mm)(P = .017). Conclusion: This study has revealed that VEGF as administered in this study promotes angiogenesis in the ACL graft and significantly reduces the stiffness of the ACL graft with increased knee laxity at 12 weeks after ACL reconstruction. Clinical Relevance: Exogenous VEGF application for ACL reconstruction can induce an increase in knee laxity and a decrease in the stiffness of the grafted tendon at least temporarily after ACL reconstruction. These potentially negative mechanical effects need to be taken into account when considering clinical use of VEGF.

Original languageEnglish
Pages (from-to)1918-1925
Number of pages8
JournalAmerican Journal of Sports Medicine
Volume34
Issue number12
DOIs
Publication statusPublished - 2006 Dec

Fingerprint

Anterior Cruciate Ligament Reconstruction
Vascular Endothelial Growth Factor A
Sheep
Knee
Transplants
Tendons
Anterior Cruciate Ligament
Confidence Intervals
Tibia
Femur
Hamstring Tendons
Fibroblasts
Phosphates

Keywords

  • Angiogenesis
  • Anterior cruciate ligament
  • Biomechanical properties
  • Vascular endothelial growth factor (VEGF)

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effects of local administration of vascular endothelial growth factor on mechanical characteristics of the semitendinosus tendon graft after anterior cruciate ligament reconstruction in sheep. / Yoshikawa, Toshikazu; Tohyama, Harukazu; Katsura, Taro; Kondo, Eiji; Kotani, Yoshihisa; Matsumoto, Hideo; Toyama, Yoshiaki; Yasuda, Kazunori.

In: American Journal of Sports Medicine, Vol. 34, No. 12, 12.2006, p. 1918-1925.

Research output: Contribution to journalArticle

Yoshikawa, Toshikazu ; Tohyama, Harukazu ; Katsura, Taro ; Kondo, Eiji ; Kotani, Yoshihisa ; Matsumoto, Hideo ; Toyama, Yoshiaki ; Yasuda, Kazunori. / Effects of local administration of vascular endothelial growth factor on mechanical characteristics of the semitendinosus tendon graft after anterior cruciate ligament reconstruction in sheep. In: American Journal of Sports Medicine. 2006 ; Vol. 34, No. 12. pp. 1918-1925.
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abstract = "Background: Vascular endothelial growth factor (VEGF) is a potent mediator of angiogenesis. Hypothesis: An application of VEGF may enhance angiogenesis in the grafted tendon in anterior cruciate ligament (ACL) reconstruction, and the application may affect mechanical characteristics of the ACL graft. Study Design: Controlled laboratory study. Methods: Eighteen sheep were divided into groups I and II. In group I, the harvested semitendinosus tendon was soaked in VEGF solution, and the right knee then underwent ACL reconstruction using this tendon. In group II, the right knee underwent identical procedures to those of group I except that the harvested tendon was soaked in phosphate-buffered saline. All animals were sacrificed 12 weeks after ACL reconstruction. Results: Histologic findings showed that newly formed vessels and infiltrative fibroblasts were more abundant in group I than in group II. The anterior-posterior translation of the knee during an anterior-posterior force of ±100 N was significantly larger in group I than in group II by 2.58 mm (95{\%} confidence interval, -1.76 mm to 1.76 mm) (P = .002). The linear stiffness of the femur-graft-tibia complex in group I was significantly lower than that in group II by 41.5 N/mm (95{\%} confidence interval, -32.2 N/mm to 32.2 N/mm)(P = .017). Conclusion: This study has revealed that VEGF as administered in this study promotes angiogenesis in the ACL graft and significantly reduces the stiffness of the ACL graft with increased knee laxity at 12 weeks after ACL reconstruction. Clinical Relevance: Exogenous VEGF application for ACL reconstruction can induce an increase in knee laxity and a decrease in the stiffness of the grafted tendon at least temporarily after ACL reconstruction. These potentially negative mechanical effects need to be taken into account when considering clinical use of VEGF.",
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T1 - Effects of local administration of vascular endothelial growth factor on mechanical characteristics of the semitendinosus tendon graft after anterior cruciate ligament reconstruction in sheep

AU - Yoshikawa, Toshikazu

AU - Tohyama, Harukazu

AU - Katsura, Taro

AU - Kondo, Eiji

AU - Kotani, Yoshihisa

AU - Matsumoto, Hideo

AU - Toyama, Yoshiaki

AU - Yasuda, Kazunori

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N2 - Background: Vascular endothelial growth factor (VEGF) is a potent mediator of angiogenesis. Hypothesis: An application of VEGF may enhance angiogenesis in the grafted tendon in anterior cruciate ligament (ACL) reconstruction, and the application may affect mechanical characteristics of the ACL graft. Study Design: Controlled laboratory study. Methods: Eighteen sheep were divided into groups I and II. In group I, the harvested semitendinosus tendon was soaked in VEGF solution, and the right knee then underwent ACL reconstruction using this tendon. In group II, the right knee underwent identical procedures to those of group I except that the harvested tendon was soaked in phosphate-buffered saline. All animals were sacrificed 12 weeks after ACL reconstruction. Results: Histologic findings showed that newly formed vessels and infiltrative fibroblasts were more abundant in group I than in group II. The anterior-posterior translation of the knee during an anterior-posterior force of ±100 N was significantly larger in group I than in group II by 2.58 mm (95% confidence interval, -1.76 mm to 1.76 mm) (P = .002). The linear stiffness of the femur-graft-tibia complex in group I was significantly lower than that in group II by 41.5 N/mm (95% confidence interval, -32.2 N/mm to 32.2 N/mm)(P = .017). Conclusion: This study has revealed that VEGF as administered in this study promotes angiogenesis in the ACL graft and significantly reduces the stiffness of the ACL graft with increased knee laxity at 12 weeks after ACL reconstruction. Clinical Relevance: Exogenous VEGF application for ACL reconstruction can induce an increase in knee laxity and a decrease in the stiffness of the grafted tendon at least temporarily after ACL reconstruction. These potentially negative mechanical effects need to be taken into account when considering clinical use of VEGF.

AB - Background: Vascular endothelial growth factor (VEGF) is a potent mediator of angiogenesis. Hypothesis: An application of VEGF may enhance angiogenesis in the grafted tendon in anterior cruciate ligament (ACL) reconstruction, and the application may affect mechanical characteristics of the ACL graft. Study Design: Controlled laboratory study. Methods: Eighteen sheep were divided into groups I and II. In group I, the harvested semitendinosus tendon was soaked in VEGF solution, and the right knee then underwent ACL reconstruction using this tendon. In group II, the right knee underwent identical procedures to those of group I except that the harvested tendon was soaked in phosphate-buffered saline. All animals were sacrificed 12 weeks after ACL reconstruction. Results: Histologic findings showed that newly formed vessels and infiltrative fibroblasts were more abundant in group I than in group II. The anterior-posterior translation of the knee during an anterior-posterior force of ±100 N was significantly larger in group I than in group II by 2.58 mm (95% confidence interval, -1.76 mm to 1.76 mm) (P = .002). The linear stiffness of the femur-graft-tibia complex in group I was significantly lower than that in group II by 41.5 N/mm (95% confidence interval, -32.2 N/mm to 32.2 N/mm)(P = .017). Conclusion: This study has revealed that VEGF as administered in this study promotes angiogenesis in the ACL graft and significantly reduces the stiffness of the ACL graft with increased knee laxity at 12 weeks after ACL reconstruction. Clinical Relevance: Exogenous VEGF application for ACL reconstruction can induce an increase in knee laxity and a decrease in the stiffness of the grafted tendon at least temporarily after ACL reconstruction. These potentially negative mechanical effects need to be taken into account when considering clinical use of VEGF.

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KW - Anterior cruciate ligament

KW - Biomechanical properties

KW - Vascular endothelial growth factor (VEGF)

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