Abstract
Effects of long-term omeprazole treatment on the process of epithelial cell proliferation and differentiation in the adult rat gastric mucosa were investigated. Animals were treated with omeprazole (25 mg/kg body weight/day) for 28 days to induce anacidity in the stomach. The treatment induced a marked decrease in the number of chief cells in the gastric mucosa and at the same time an increase in that of immature pepsinogen-producing cells expressing class III mucin. This was accompanied by a decrease to 60% and 10% of the control values in the mucosal levels of pepsinogen and its mRNA, respectively. Moreover, the expression of cathepsin E in surface mucous cells was reduced. Cell proliferation studies revealed that the rate of bromodeoxyuridine-labeled cells was increased by omeprazole. The above-described changes were reversed by cessation of the treatment and they were not caused by the omeprazole-treatment at a dose which does not induce anacidity in the stomach. These results suggest that long-term omeprazole treatment reversibly increases the epithelial cell proliferation and suppresses its differentiation in the adult rat gastric mucosa probably by altering the acidic environment specific for the stomach.
| Original language | English |
|---|---|
| Pages (from-to) | 861-868 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 214 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1995 Sep 25 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
- Biophysics
- Biochemistry
Cite this
Effects of Long-Term Omeprazole Treatment on Adult Rat Gastric Mucosa - Enhancement of the Epithelial Cell Proliferation and Suppression of Its Differentiation. / Kakei, N.; Ichinose, M.; Tatematsu, M.; Shimizu, M.; Oka, M.; Yahagi, Naohisa; Matsushima, M.; Kurokawa, K.; Yonezawa, S.; Furihata, C.; Shiokawa, K.; Kageyama, T.; Miki, K.; Fukamachi, H.
In: Biochemical and Biophysical Research Communications, Vol. 214, No. 3, 25.09.1995, p. 861-868.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of Long-Term Omeprazole Treatment on Adult Rat Gastric Mucosa - Enhancement of the Epithelial Cell Proliferation and Suppression of Its Differentiation
AU - Kakei, N.
AU - Ichinose, M.
AU - Tatematsu, M.
AU - Shimizu, M.
AU - Oka, M.
AU - Yahagi, Naohisa
AU - Matsushima, M.
AU - Kurokawa, K.
AU - Yonezawa, S.
AU - Furihata, C.
AU - Shiokawa, K.
AU - Kageyama, T.
AU - Miki, K.
AU - Fukamachi, H.
PY - 1995/9/25
Y1 - 1995/9/25
N2 - Effects of long-term omeprazole treatment on the process of epithelial cell proliferation and differentiation in the adult rat gastric mucosa were investigated. Animals were treated with omeprazole (25 mg/kg body weight/day) for 28 days to induce anacidity in the stomach. The treatment induced a marked decrease in the number of chief cells in the gastric mucosa and at the same time an increase in that of immature pepsinogen-producing cells expressing class III mucin. This was accompanied by a decrease to 60% and 10% of the control values in the mucosal levels of pepsinogen and its mRNA, respectively. Moreover, the expression of cathepsin E in surface mucous cells was reduced. Cell proliferation studies revealed that the rate of bromodeoxyuridine-labeled cells was increased by omeprazole. The above-described changes were reversed by cessation of the treatment and they were not caused by the omeprazole-treatment at a dose which does not induce anacidity in the stomach. These results suggest that long-term omeprazole treatment reversibly increases the epithelial cell proliferation and suppresses its differentiation in the adult rat gastric mucosa probably by altering the acidic environment specific for the stomach.
AB - Effects of long-term omeprazole treatment on the process of epithelial cell proliferation and differentiation in the adult rat gastric mucosa were investigated. Animals were treated with omeprazole (25 mg/kg body weight/day) for 28 days to induce anacidity in the stomach. The treatment induced a marked decrease in the number of chief cells in the gastric mucosa and at the same time an increase in that of immature pepsinogen-producing cells expressing class III mucin. This was accompanied by a decrease to 60% and 10% of the control values in the mucosal levels of pepsinogen and its mRNA, respectively. Moreover, the expression of cathepsin E in surface mucous cells was reduced. Cell proliferation studies revealed that the rate of bromodeoxyuridine-labeled cells was increased by omeprazole. The above-described changes were reversed by cessation of the treatment and they were not caused by the omeprazole-treatment at a dose which does not induce anacidity in the stomach. These results suggest that long-term omeprazole treatment reversibly increases the epithelial cell proliferation and suppresses its differentiation in the adult rat gastric mucosa probably by altering the acidic environment specific for the stomach.
UR - http://www.scopus.com/inward/record.url?scp=0028807853&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028807853&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1995.2366
DO - 10.1006/bbrc.1995.2366
M3 - Article
C2 - 7575556
AN - SCOPUS:0028807853
VL - 214
SP - 861
EP - 868
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -