Abstract
Effects of long-term omeprazole treatment on the process of epithelial cell proliferation and differentiation in the adult rat gastric mucosa were investigated. Animals were treated with omeprazole (25 mg/kg body weight/day) for 28 days to induce anacidity in the stomach. The treatment induced a marked decrease in the number of chief cells in the gastric mucosa and at the same time an increase in that of immature pepsinogen-producing cells expressing class III mucin. This was accompanied by a decrease to 60% and 10% of the control values in the mucosal levels of pepsinogen and its mRNA, respectively. Moreover, the expression of cathepsin E in surface mucous cells was reduced. Cell proliferation studies revealed that the rate of bromodeoxyuridine-labeled cells was increased by omeprazole. The above-described changes were reversed by cessation of the treatment and they were not caused by the omeprazole-treatment at a dose which does not induce anacidity in the stomach. These results suggest that long-term omeprazole treatment reversibly increases the epithelial cell proliferation and suppresses its differentiation in the adult rat gastric mucosa probably by altering the acidic environment specific for the stomach.
Original language | English |
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Pages (from-to) | 861-868 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 214 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1995 Sep 25 |
Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
- Biophysics
- Biochemistry
Cite this
Effects of Long-Term Omeprazole Treatment on Adult Rat Gastric Mucosa - Enhancement of the Epithelial Cell Proliferation and Suppression of Its Differentiation. / Kakei, N.; Ichinose, M.; Tatematsu, M.; Shimizu, M.; Oka, M.; Yahagi, Naohisa; Matsushima, M.; Kurokawa, K.; Yonezawa, S.; Furihata, C.; Shiokawa, K.; Kageyama, T.; Miki, K.; Fukamachi, H.
In: Biochemical and Biophysical Research Communications, Vol. 214, No. 3, 25.09.1995, p. 861-868.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of Long-Term Omeprazole Treatment on Adult Rat Gastric Mucosa - Enhancement of the Epithelial Cell Proliferation and Suppression of Its Differentiation
AU - Kakei, N.
AU - Ichinose, M.
AU - Tatematsu, M.
AU - Shimizu, M.
AU - Oka, M.
AU - Yahagi, Naohisa
AU - Matsushima, M.
AU - Kurokawa, K.
AU - Yonezawa, S.
AU - Furihata, C.
AU - Shiokawa, K.
AU - Kageyama, T.
AU - Miki, K.
AU - Fukamachi, H.
PY - 1995/9/25
Y1 - 1995/9/25
N2 - Effects of long-term omeprazole treatment on the process of epithelial cell proliferation and differentiation in the adult rat gastric mucosa were investigated. Animals were treated with omeprazole (25 mg/kg body weight/day) for 28 days to induce anacidity in the stomach. The treatment induced a marked decrease in the number of chief cells in the gastric mucosa and at the same time an increase in that of immature pepsinogen-producing cells expressing class III mucin. This was accompanied by a decrease to 60% and 10% of the control values in the mucosal levels of pepsinogen and its mRNA, respectively. Moreover, the expression of cathepsin E in surface mucous cells was reduced. Cell proliferation studies revealed that the rate of bromodeoxyuridine-labeled cells was increased by omeprazole. The above-described changes were reversed by cessation of the treatment and they were not caused by the omeprazole-treatment at a dose which does not induce anacidity in the stomach. These results suggest that long-term omeprazole treatment reversibly increases the epithelial cell proliferation and suppresses its differentiation in the adult rat gastric mucosa probably by altering the acidic environment specific for the stomach.
AB - Effects of long-term omeprazole treatment on the process of epithelial cell proliferation and differentiation in the adult rat gastric mucosa were investigated. Animals were treated with omeprazole (25 mg/kg body weight/day) for 28 days to induce anacidity in the stomach. The treatment induced a marked decrease in the number of chief cells in the gastric mucosa and at the same time an increase in that of immature pepsinogen-producing cells expressing class III mucin. This was accompanied by a decrease to 60% and 10% of the control values in the mucosal levels of pepsinogen and its mRNA, respectively. Moreover, the expression of cathepsin E in surface mucous cells was reduced. Cell proliferation studies revealed that the rate of bromodeoxyuridine-labeled cells was increased by omeprazole. The above-described changes were reversed by cessation of the treatment and they were not caused by the omeprazole-treatment at a dose which does not induce anacidity in the stomach. These results suggest that long-term omeprazole treatment reversibly increases the epithelial cell proliferation and suppresses its differentiation in the adult rat gastric mucosa probably by altering the acidic environment specific for the stomach.
UR - http://www.scopus.com/inward/record.url?scp=0028807853&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028807853&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1995.2366
DO - 10.1006/bbrc.1995.2366
M3 - Article
C2 - 7575556
AN - SCOPUS:0028807853
VL - 214
SP - 861
EP - 868
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -