Effects of Long-Term Omeprazole Treatment on Adult Rat Gastric Mucosa - Enhancement of the Epithelial Cell Proliferation and Suppression of Its Differentiation

Nobuyuki Kakei, Masao Ichinose, Masac Tatematsu, Miyuki Shimizu, Masashi Oka Oka, Naohisa Yahagi, Masashi Matsushima, Kiyoshi Kurokawa, Satoshi Yonezawa, Chie Furihata, Koichiro Shiokawa, Takashi Kageyama, Kazumasa Miki, Fliroshi Fukamachi

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17 Citations (Scopus)

Abstract

Effects of long-term omeprazole treatment on the process of epithelial cell proliferation and differentiation in the adult rat gastric mucosa were investigated. Animals were treated with omeprazole (25 mg/kg body weight/day) for 28 days to induce anacidity in the stomach. The treatment induced a marked decrease in the number of chief cells in the gastric mucosa and at the same time an increase in that of immature pepsinogen-producing cells expressing class III mucin. This was accompanied by a decrease to 60% and 10% of the control values in the mucosal levels of pepsinogen and its mRNA, respectively. Moreover, the expression of cathepsin E in surface mucous cells was reduced. Cell proliferation studies revealed that the rate of bromodeoxyuridine-labeled cells was increased by omeprazole. The above-described changes were reversed by cessation of the treatment and they were not caused by the omeprazole-treatment at a dose which does not induce anacidity in the stomach. These results suggest that long-term omeprazole treatment reversibly increases the epithelial cell proliferation and suppresses its differentiation in the adult rat gastric mucosa probably by altering the acidic environment specific for the stomach.

Original languageEnglish
Pages (from-to)861-868
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume214
Issue number3
DOIs
Publication statusPublished - 1995 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Kakei, N., Ichinose, M., Tatematsu, M., Shimizu, M., Oka, M. O., Yahagi, N., Matsushima, M., Kurokawa, K., Yonezawa, S., Furihata, C., Shiokawa, K., Kageyama, T., Miki, K., & Fukamachi, F. (1995). Effects of Long-Term Omeprazole Treatment on Adult Rat Gastric Mucosa - Enhancement of the Epithelial Cell Proliferation and Suppression of Its Differentiation. Biochemical and Biophysical Research Communications, 214(3), 861-868. https://doi.org/10.1006/bbrc.1995.2366