Effects of long-term oral administration of arachidonic acid and docosahexaenoic acid on the immune functions of young rats

Sachiko Juman, Michio Hashimoto, Masanori Katakura, Takayuki Inoue, Yoko Tanabe, Makoto Arita, Tomohiro Miki, Osamu Shido

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Natural killer (NK) cells have many functional activities, including cytotoxicity and the capacity to produce cytokines and chemokines. NK cell activity is regulated partly by eicosanoids, which are produced from arachidonic acid (ARA) and eicosapentaenoic (EPA) acid. In this study, we investigated the effects of long-term therapy with ARA or docosahexaenoic acid (DHA) on the cytotoxic effects of the NK cells of young rats, which were fed on a nonfish oil diet for two generations. Control oil, ARA (240 mg/kg BW/day) or DHA (240 mg/kg BW/day) were orally administrated to the rats for 13 weeks before determining the cytotoxic activity of NK cells from the spleen against YAC-1 mouse lymphoma cell line, as well as the plasma levels of docosanoids or eicosanoids and inflammatory cytokines. Long-term ARA administration significantly suppressed the cytotoxic activity of NK cells. Moreover, ARA administration significantly increased the plasma levels of ARA, prostaglandin (PG) E2, and PGD2. However, DHA administration did not produce any different effects compared with those in the control rats. Furthermore, the inflammatory cytokine levels were not affected by the administration of ARA or DHA. These results suggest that long-term ARA administration has an inhibitory effect on the tumor cytotoxicity of NK cells in rat spleen lymphocytes owing to the enhanced synthesis of PGE2 and PGD2 from ARA because of the elevated plasma ARA levels in young rats.

Original languageEnglish
Pages (from-to)1949-1961
Number of pages13
JournalNutrients
Volume5
Issue number6
DOIs
Publication statusPublished - 2013 May 29
Externally publishedYes

Fingerprint

Docosahexaenoic Acids
docosahexaenoic acid
arachidonic acid
Arachidonic Acid
oral administration
Oral Administration
long term effects
natural killer cells
Natural Killer Cells
rats
cytotoxicity
Prostaglandin D2
cytokines
eicosanoids
Eicosanoids
Cytokines
Dinoprostone
Oils
spleen
Spleen

Keywords

  • Eicosanoids
  • Natural killer cell
  • Polyunsaturated fatty acid
  • Prostaglandin D
  • Prostaglandin E

ASJC Scopus subject areas

  • Food Science

Cite this

Effects of long-term oral administration of arachidonic acid and docosahexaenoic acid on the immune functions of young rats. / Juman, Sachiko; Hashimoto, Michio; Katakura, Masanori; Inoue, Takayuki; Tanabe, Yoko; Arita, Makoto; Miki, Tomohiro; Shido, Osamu.

In: Nutrients, Vol. 5, No. 6, 29.05.2013, p. 1949-1961.

Research output: Contribution to journalArticle

Juman, S, Hashimoto, M, Katakura, M, Inoue, T, Tanabe, Y, Arita, M, Miki, T & Shido, O 2013, 'Effects of long-term oral administration of arachidonic acid and docosahexaenoic acid on the immune functions of young rats', Nutrients, vol. 5, no. 6, pp. 1949-1961. https://doi.org/10.3390/nu5061949
Juman, Sachiko ; Hashimoto, Michio ; Katakura, Masanori ; Inoue, Takayuki ; Tanabe, Yoko ; Arita, Makoto ; Miki, Tomohiro ; Shido, Osamu. / Effects of long-term oral administration of arachidonic acid and docosahexaenoic acid on the immune functions of young rats. In: Nutrients. 2013 ; Vol. 5, No. 6. pp. 1949-1961.
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