Effects of parathyroid hormone (PTH) and PTH-related peptide on expressions of matrix metalloproteinase- 2, -3, and -9 in growth plate chondrocyte cultures

Yoshie Kawashima-Ohya, Hisashi Satakeda, Yoshihiro Kuruta, Takeshi Kawamoto, Weiqun Yan, Yasumasa Akagawa, Taro Hayakawa, Mitsuhide Noshiro, Yasunori Okada, Shigeo Nakamura, Yukio Kato

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Abstract

The roles of PTH and PTH-related peptide (PTH-rp) in the expression of matrix metalloproteinases (MMPs) during endochondral bone formation were investigated, using various cartilages obtained from young rabbits and rabbit chondrocyte cultures. Immunohistochemical, immunoblotting, zymographical, and/or Northern blot analyses showed that MMP-2 and -9 levels were much higher in the growth plate than in permanent cartilage in vivo. In growth plate chondrocyte cultures, PTH, PTH-rp, and (Bu)2cAMP increased the amount of MMP-2 present in the culture medium, as revealed by zymograms and immunoblots, whereas the other tested growth factors or cytokines, including bone morphogenetic protein-2 and interleukin-1, did not increase the MMP-2 level. PTH also increased the MMP-2 messenger RNA level within 24 h. In addition, PTH increased MMP-3 and -9 levels in the growth plate chondrocyte cultures. However, in articular chondrocyte cultures, PTH had little effect on the levels of MMP-2, -3, and -9. In contrast to PTH, interleukin-1 induced MMP-3 and -9, but not MMP-2, in growth plate and articular chondrocytes. These findings suggest that in ossifying cartilage, PTH/PTH-rp plays a pivotal role in the induction of various MMPs, including MMP-2 (which is considered to be a constitutive enzyme), and that PTH/PTH-rp is involved in the control of cartilage-matrix degradation during endochondral bone formation.

Original languageEnglish
Pages (from-to)2120-2127
Number of pages8
JournalEndocrinology
Volume139
Issue number4
Publication statusPublished - 1998

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Parathyroid Hormone-Related Protein
Matrix Metalloproteinase 3
Growth Plate
Matrix Metalloproteinase 2
Chondrocytes
Parathyroid Hormone
Cartilage
Matrix Metalloproteinase 9
Matrix Metalloproteinases
Interleukin-1
Osteogenesis
Joints
Rabbits
Bone Morphogenetic Protein 2
Immunoblotting
Northern Blotting
Culture Media
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Kawashima-Ohya, Y., Satakeda, H., Kuruta, Y., Kawamoto, T., Yan, W., Akagawa, Y., ... Kato, Y. (1998). Effects of parathyroid hormone (PTH) and PTH-related peptide on expressions of matrix metalloproteinase- 2, -3, and -9 in growth plate chondrocyte cultures. Endocrinology, 139(4), 2120-2127.

Effects of parathyroid hormone (PTH) and PTH-related peptide on expressions of matrix metalloproteinase- 2, -3, and -9 in growth plate chondrocyte cultures. / Kawashima-Ohya, Yoshie; Satakeda, Hisashi; Kuruta, Yoshihiro; Kawamoto, Takeshi; Yan, Weiqun; Akagawa, Yasumasa; Hayakawa, Taro; Noshiro, Mitsuhide; Okada, Yasunori; Nakamura, Shigeo; Kato, Yukio.

In: Endocrinology, Vol. 139, No. 4, 1998, p. 2120-2127.

Research output: Contribution to journalArticle

Kawashima-Ohya, Y, Satakeda, H, Kuruta, Y, Kawamoto, T, Yan, W, Akagawa, Y, Hayakawa, T, Noshiro, M, Okada, Y, Nakamura, S & Kato, Y 1998, 'Effects of parathyroid hormone (PTH) and PTH-related peptide on expressions of matrix metalloproteinase- 2, -3, and -9 in growth plate chondrocyte cultures', Endocrinology, vol. 139, no. 4, pp. 2120-2127.
Kawashima-Ohya, Yoshie ; Satakeda, Hisashi ; Kuruta, Yoshihiro ; Kawamoto, Takeshi ; Yan, Weiqun ; Akagawa, Yasumasa ; Hayakawa, Taro ; Noshiro, Mitsuhide ; Okada, Yasunori ; Nakamura, Shigeo ; Kato, Yukio. / Effects of parathyroid hormone (PTH) and PTH-related peptide on expressions of matrix metalloproteinase- 2, -3, and -9 in growth plate chondrocyte cultures. In: Endocrinology. 1998 ; Vol. 139, No. 4. pp. 2120-2127.
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