Effects of recombinant murine granulocyte colony-stimulating factor on granulocyte-macrophage and blast colony formation

T. Suda, J. Suda, S. Kajigaya, S. Nagata, S. Asano, M. Saito, Y. Miura

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We performed the present study to define the in vitro hemopoietic activity of murine recombinant (r) granulocyte colony-stimulating factor (G-CSF) using murine hemopoietic culture systems of normal bone marrow cells, fetal liver cells, and spleen cells of 5-fluorouracil (FU)-treated mice. Recombinant G-CSF supported only neutrophil and/or macrophage colony formation by normal bone marrow cells. It did not enhance the formation of erythroid bursts in the fetal liver cell assay, but interleukin-3 (IL-3) did. Paradoxicaly, rG-CSF could support the colony formation of multilineage colonies as well as blast colonies from the spleen cells of 5-FU-treated mice, while r-granulocyte-macrophage colony-stimulating factor (GM-CSF) and r-erythropoietin (Ep) did not. When blast colonies, formed in the presence of G-CSF, were replated to dishes containing IL-3, they were able to differentiate along multilineage pathways, However, when they were replated to dishes containing rG-CSF, they could differentiate only into neutrophils and macrophages. Single cells transferred from blast colonies formed only neutrophil-macrophage colonies. These data indicate that rG-CSF had a direct effect on the growth and development of GM progenitors at a late stage and a significant effect on multipotential hemopoietic precursors. Although it remains to be clarified how G-CSF acts on multipotential stem cells, this unique effect is important in the understanding of its pluripotent hemopoietic activity in vivo.

Original languageEnglish
Pages (from-to)958-965
Number of pages8
JournalExperimental Hematology
Volume15
Issue number9
Publication statusPublished - 1987

Fingerprint

Granulocyte Colony-Stimulating Factor
Granulocytes
Macrophages
Neutrophils
Interleukin-3
Fluorouracil
Bone Marrow Cells
Spleen
Liver
Granulocyte-Macrophage Colony-Stimulating Factor
Erythropoietin
Growth and Development
Stem Cells

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Suda, T., Suda, J., Kajigaya, S., Nagata, S., Asano, S., Saito, M., & Miura, Y. (1987). Effects of recombinant murine granulocyte colony-stimulating factor on granulocyte-macrophage and blast colony formation. Experimental Hematology, 15(9), 958-965.

Effects of recombinant murine granulocyte colony-stimulating factor on granulocyte-macrophage and blast colony formation. / Suda, T.; Suda, J.; Kajigaya, S.; Nagata, S.; Asano, S.; Saito, M.; Miura, Y.

In: Experimental Hematology, Vol. 15, No. 9, 1987, p. 958-965.

Research output: Contribution to journalArticle

Suda, T, Suda, J, Kajigaya, S, Nagata, S, Asano, S, Saito, M & Miura, Y 1987, 'Effects of recombinant murine granulocyte colony-stimulating factor on granulocyte-macrophage and blast colony formation', Experimental Hematology, vol. 15, no. 9, pp. 958-965.
Suda, T. ; Suda, J. ; Kajigaya, S. ; Nagata, S. ; Asano, S. ; Saito, M. ; Miura, Y. / Effects of recombinant murine granulocyte colony-stimulating factor on granulocyte-macrophage and blast colony formation. In: Experimental Hematology. 1987 ; Vol. 15, No. 9. pp. 958-965.
@article{43e4c1930e734ce0b5e74e0d14322a07,
title = "Effects of recombinant murine granulocyte colony-stimulating factor on granulocyte-macrophage and blast colony formation",
abstract = "We performed the present study to define the in vitro hemopoietic activity of murine recombinant (r) granulocyte colony-stimulating factor (G-CSF) using murine hemopoietic culture systems of normal bone marrow cells, fetal liver cells, and spleen cells of 5-fluorouracil (FU)-treated mice. Recombinant G-CSF supported only neutrophil and/or macrophage colony formation by normal bone marrow cells. It did not enhance the formation of erythroid bursts in the fetal liver cell assay, but interleukin-3 (IL-3) did. Paradoxicaly, rG-CSF could support the colony formation of multilineage colonies as well as blast colonies from the spleen cells of 5-FU-treated mice, while r-granulocyte-macrophage colony-stimulating factor (GM-CSF) and r-erythropoietin (Ep) did not. When blast colonies, formed in the presence of G-CSF, were replated to dishes containing IL-3, they were able to differentiate along multilineage pathways, However, when they were replated to dishes containing rG-CSF, they could differentiate only into neutrophils and macrophages. Single cells transferred from blast colonies formed only neutrophil-macrophage colonies. These data indicate that rG-CSF had a direct effect on the growth and development of GM progenitors at a late stage and a significant effect on multipotential hemopoietic precursors. Although it remains to be clarified how G-CSF acts on multipotential stem cells, this unique effect is important in the understanding of its pluripotent hemopoietic activity in vivo.",
author = "T. Suda and J. Suda and S. Kajigaya and S. Nagata and S. Asano and M. Saito and Y. Miura",
year = "1987",
language = "English",
volume = "15",
pages = "958--965",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier Inc.",
number = "9",

}

TY - JOUR

T1 - Effects of recombinant murine granulocyte colony-stimulating factor on granulocyte-macrophage and blast colony formation

AU - Suda, T.

AU - Suda, J.

AU - Kajigaya, S.

AU - Nagata, S.

AU - Asano, S.

AU - Saito, M.

AU - Miura, Y.

PY - 1987

Y1 - 1987

N2 - We performed the present study to define the in vitro hemopoietic activity of murine recombinant (r) granulocyte colony-stimulating factor (G-CSF) using murine hemopoietic culture systems of normal bone marrow cells, fetal liver cells, and spleen cells of 5-fluorouracil (FU)-treated mice. Recombinant G-CSF supported only neutrophil and/or macrophage colony formation by normal bone marrow cells. It did not enhance the formation of erythroid bursts in the fetal liver cell assay, but interleukin-3 (IL-3) did. Paradoxicaly, rG-CSF could support the colony formation of multilineage colonies as well as blast colonies from the spleen cells of 5-FU-treated mice, while r-granulocyte-macrophage colony-stimulating factor (GM-CSF) and r-erythropoietin (Ep) did not. When blast colonies, formed in the presence of G-CSF, were replated to dishes containing IL-3, they were able to differentiate along multilineage pathways, However, when they were replated to dishes containing rG-CSF, they could differentiate only into neutrophils and macrophages. Single cells transferred from blast colonies formed only neutrophil-macrophage colonies. These data indicate that rG-CSF had a direct effect on the growth and development of GM progenitors at a late stage and a significant effect on multipotential hemopoietic precursors. Although it remains to be clarified how G-CSF acts on multipotential stem cells, this unique effect is important in the understanding of its pluripotent hemopoietic activity in vivo.

AB - We performed the present study to define the in vitro hemopoietic activity of murine recombinant (r) granulocyte colony-stimulating factor (G-CSF) using murine hemopoietic culture systems of normal bone marrow cells, fetal liver cells, and spleen cells of 5-fluorouracil (FU)-treated mice. Recombinant G-CSF supported only neutrophil and/or macrophage colony formation by normal bone marrow cells. It did not enhance the formation of erythroid bursts in the fetal liver cell assay, but interleukin-3 (IL-3) did. Paradoxicaly, rG-CSF could support the colony formation of multilineage colonies as well as blast colonies from the spleen cells of 5-FU-treated mice, while r-granulocyte-macrophage colony-stimulating factor (GM-CSF) and r-erythropoietin (Ep) did not. When blast colonies, formed in the presence of G-CSF, were replated to dishes containing IL-3, they were able to differentiate along multilineage pathways, However, when they were replated to dishes containing rG-CSF, they could differentiate only into neutrophils and macrophages. Single cells transferred from blast colonies formed only neutrophil-macrophage colonies. These data indicate that rG-CSF had a direct effect on the growth and development of GM progenitors at a late stage and a significant effect on multipotential hemopoietic precursors. Although it remains to be clarified how G-CSF acts on multipotential stem cells, this unique effect is important in the understanding of its pluripotent hemopoietic activity in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0023612482&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023612482&partnerID=8YFLogxK

M3 - Article

C2 - 3498640

AN - SCOPUS:0023612482

VL - 15

SP - 958

EP - 965

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 9

ER -