Effects of serine protease inhibitors on oxyradical burst from phagocytizing neutrophils--analysis by chemiluminescence counting and its microscopic imaging.

M. Suematsu, S. Houzawa, S. Miura, H. Nagata, T. Kitahora, T. Morishita, C. Oshio, M. Tsuchiya

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Reaction difference of oxyradical generation and luminol-dependent photoemission of zymosan- and phorbol ester-treated neutrophils were investigated using a conventional photomultiplier and ultrasensitive photonic imaging technique. Zymosan-treated cells released a concentrated photonic burst corresponding to the cellular distribution. In contrast, phorbol ester-treated cells produced a negligible level of photoemission, and the additional application of Ca2+ ionophore enhanced the photonic burst, which was gradually spread out into extracellular space. Serine protease inhibitors did not attenuate PMA-induced chemiluminescence but did attenuate zymosan-induced chemiluminescence. This suggests the involvement of serine protease in the respiratory burst of phagocytizing neutrophils.

Original languageEnglish
Pages (from-to)531-534
Number of pages4
JournalJournal of bioluminescence and chemiluminescence
Volume4
Issue number1
DOIs
Publication statusPublished - 1989 Jul

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)

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