Effects of some diethylstilbestrol metabolites and analogs on cytotoxicity and aneuploidy induction in Chinese hamster V79 cells

T. Oda, M. Watanuki, Y. Sakakibara, Y. Sato

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have previously reported the inhibitory effects of diethylstilbestrol (1) and optically active indenestrol derivatives on microtubule polymerization in vitro and their disruptive effect on cytoplasmic microtubules and cytotoxicity in cultured Chinese hamster V79 cells. In the present study, the cytotoxicities of (±)-diethylstilbestrol oxide (2), (+)- , (-)- and (±)-monomethyl ethers (4) of 2, (±)-dimethyl ether (5) of 2, diethylstilbestrol pinacolone (3), E,E-dienestrol (6), Z,Z-dienestrol (7), meso-hexestrol (8), a mixture of (1R,1'S)4-hydroxyhexestrol and (1R,1'S)4'- hydroxyhexestrol (9), and the 4-hydroxy derivative (10) of diethylstilbestrol dimethyl ether were investigated in Chinese hamster V79 cells. The results indicated that the cytotoxic activity of 10 was the strongest of the compounds tested, although its activity was the almost same as that of 1. Moreover, as the activity of (-)-4 was greater than those of 2 and 1 monomethyl ethers, the effect of 4 on cytotoxic activities was elucidated. In conclusion, the present results indicate that the cytotoxic activities of hydroxylated metabolites are greater than those of each mother compound, although epoxidation of 1 leads to a product which can be broken down more readily than the parent compound.

Original languageEnglish
Pages (from-to)1435-1438
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume18
Issue number10
Publication statusPublished - 1995

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Dienestrol
Diethylstilbestrol
Ethers
Aneuploidy
Cricetulus
Microtubules
Hexestrol
Polymerization
Oxides

Keywords

  • aneuploidy
  • cytotoxicity
  • diethylstilbestrol metabolite
  • diethylstilbestrol oxide

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Effects of some diethylstilbestrol metabolites and analogs on cytotoxicity and aneuploidy induction in Chinese hamster V79 cells. / Oda, T.; Watanuki, M.; Sakakibara, Y.; Sato, Y.

In: Biological and Pharmaceutical Bulletin, Vol. 18, No. 10, 1995, p. 1435-1438.

Research output: Contribution to journalArticle

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AU - Sato, Y.

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