Effects of supersonic treatment on lactoferrin separation from whey waste using reversed micellar system

Hiroyoshi Inoue, Mariko Yamasaki, D. Shanthana Lakshmi, Toshiyuki Kojo, Sho Ichi Yamagishi

Research output: Contribution to journalArticle

Abstract

A cationic oleic acid reversed micellar system was used to simplify and enhance the purification of bovine lactoferrin (Lfn) from whey. The rate of Lfn extraction by the application of 30 kHz supersonic treatment to the organic solvent phase (P < 0.01) was by 1.5 time higher compared to non-supersonic treatment. An increase in water content and the light scatter were observed in the organic phase when the supersonic treatment was applied. An increase in the efficiency of Lfn extraction by supersonic treatment of an organic phase was attributable to increased water dispersion in the organic solvent phase and an increase in the orientation of the cationic oleic acid towards microparticles of water in organic solvent phase.

Original languageEnglish
Pages (from-to)5-12
Number of pages8
JournalEnvironment Protection Engineering
Volume31
Issue number3-4
Publication statusPublished - 2005 Dec 1
Externally publishedYes

Keywords

  • Lactoferrin
  • Lipid
  • Reversed micellar system
  • Supersonication
  • Whey

ASJC Scopus subject areas

  • Environmental Engineering
  • Waste Management and Disposal

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