Effects of tumor necrosis factor-α on the synthesis of DNA, the secretion of matrix metalloproteinases/tissue inhibitors of metalloproteinases, and the activity of invasive migration in cultured vascular smooth muscle cells

Toshiyuki Kaji, Shouichi Hiraga, Chika Yamamoto, Yasuyuki Fujiwara, Yoshimichi Ueda, Fumiko Zisaki, Kazushi Iwata, Yasunori Okada, Shogo Katsuda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

To address the question of whether or not tumor necrosis factor-α (TNF-α) regulates the functions of vascular smooth muscle cells (SMCs), dense or sparse cultures of the cells derived from human aorta were treated with TNF-α or TNF-α neutralizing antibody (TNF-α Ab). The incorporation of [3H]thymidine into the acid-insoluble fraction of SMCs was significantly inhibited by TNF-α, but stimulated by TNF-α Ab only when the cells had a high cell density. TNF-α significantly increased the accumulation of matrix metalloproteinase-1 and -3 (MMT-1 and -3, respectively) in the conditioned medium of dense SMCs, but does not affect that of tissue inhibitors of metalloproteinases-1 (TIMP-1); MMP-9 and TIMP-2 were undetectable. The invasive migration of SMCs determined by a Transwell system was stimulated by neither TNF-α nor TNF-α Ab. Taking these results together, it is suggested that TNF-α regulates DNA and MMP synthesis in dense SMCs but does not affect their invasive migration.

Original languageEnglish
Pages (from-to)354-358
Number of pages5
JournalJournal of Health Science
Volume48
Issue number4
DOIs
Publication statusPublished - 2002 Aug 1

Keywords

  • Atherosclerosis
  • Matrix metalloproteinase
  • Migration
  • Proliferation
  • Tumor necrosis factor alpha
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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