Effects of X irradiation on metabolism of proteoglycans

Akitoshi Jikko, Hiroko Hiranuma, Masahiro Iwamoto, Yukio Kato, Yasunori Okada, Hajime Fuchihata

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effects of X irradiation on matrix formation by growth-plate and articular chondrocytes, as reflected by metabolism of proteoglycans and type II collagen, were examined in a rabbit chondrocyte culture system. Irradiation with 1 to 10 Gy selectively inhibited synthesis of proteoglycans (incorporation of [35S]sulfate) depending on the stage of differentiation of the irradiated cells; however, synthesis of type II collagen was not affected. Irradiation of an immature culture, in which chondrocytes had just reached confluence, suppressed incorporation of [35S]sulfate into the glycosaminoglycan, 10 Gy inducing approximately 45-50% inhibition. In contrast, the irradiation of mature cultures, in which chondrocytes had already secreted extensive cartilage matrix, did not affect the rate of synthesis of proteoglycans (incorporation of [35S]sulfate). We also found that here irradiation stimulated the degradation of proteoglycans, but with the effect differing in growth-plate chondrocytes and articular chondrocytes. In growth-plate chondrocytes, cleavage from a site close to the G1 globular domain induced by 10 Gy enhanced the release of 35S-labeled proteoglycans into the medium, whereas in articular chondrocytes, irradiation had only marginal effects on the release of 35S-labeled proteoglycans. Our results show that irradiation with 1-10 Gy impaired proteoglycan metabolism in cartilage, with differing effects according to the stage of cell differentiation and the type of chondrocyte.

Original languageEnglish
Pages (from-to)93-99
Number of pages7
JournalRadiation Research
Volume146
Issue number1
DOIs
Publication statusPublished - 1996 Jul

Fingerprint

proteoglycans
chondrocytes
metabolism
Proteoglycans
Chondrocytes
irradiation
growth plate
Growth Plate
sulfates
cartilage
Sulfates
collagens
Collagen Type II
Joints
synthesis
Cartilage
collagen
Cell Differentiation
rabbits
matrices

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Jikko, A., Hiranuma, H., Iwamoto, M., Kato, Y., Okada, Y., & Fuchihata, H. (1996). Effects of X irradiation on metabolism of proteoglycans. Radiation Research, 146(1), 93-99. https://doi.org/10.2307/3579401

Effects of X irradiation on metabolism of proteoglycans. / Jikko, Akitoshi; Hiranuma, Hiroko; Iwamoto, Masahiro; Kato, Yukio; Okada, Yasunori; Fuchihata, Hajime.

In: Radiation Research, Vol. 146, No. 1, 07.1996, p. 93-99.

Research output: Contribution to journalArticle

Jikko, A, Hiranuma, H, Iwamoto, M, Kato, Y, Okada, Y & Fuchihata, H 1996, 'Effects of X irradiation on metabolism of proteoglycans', Radiation Research, vol. 146, no. 1, pp. 93-99. https://doi.org/10.2307/3579401
Jikko A, Hiranuma H, Iwamoto M, Kato Y, Okada Y, Fuchihata H. Effects of X irradiation on metabolism of proteoglycans. Radiation Research. 1996 Jul;146(1):93-99. https://doi.org/10.2307/3579401
Jikko, Akitoshi ; Hiranuma, Hiroko ; Iwamoto, Masahiro ; Kato, Yukio ; Okada, Yasunori ; Fuchihata, Hajime. / Effects of X irradiation on metabolism of proteoglycans. In: Radiation Research. 1996 ; Vol. 146, No. 1. pp. 93-99.
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