Abstract
The advent of microarray technology, coupled with the availability of mouse cDNA collections derived specifically from preimplantation embryos, helps to provide global gene expression profiles for the earliest stages of development. However, to determine the functions of the large numbers of genes of interest, massive systematic functional assays such as gene 'knockdown' experiments are required. As a first step, the relative suppression of blastocyst formation by differentially-modified antisense oligonucleotides to E-cadherin was assayed. The injection of 2′-methoxyethoxy (2′-MOE)-modified oligonucleotides blocked the formation of blastocysts in two-thirds of embryos, whereas the injection of either control missense 2′-MOE-oligonucleotides, or oligonucleotides with a Morpholino modification, had no significant effect on embryonic development. Thus, the 2′-MOE-modified antisense oligonucleotides are candidates for effective examination of roles of large numbers of genes during early embryological development.
| Original language | English |
|---|---|
| Pages (from-to) | 318-322 |
| Number of pages | 5 |
| Journal | Reproductive BioMedicine Online |
| Volume | 6 |
| Issue number | 3 |
| Publication status | Published - 2003 Apr |
| Externally published | Yes |
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Keywords
- Antisense oligonucleotide
- Embryo
- Gene 'knockdown'
- Preimplantation development
ASJC Scopus subject areas
- Obstetrics and Gynaecology
Cite this
Efficacy of 2-methoxyethoxy-modified antisense oligonucleotides for the study of mouse preimplantation development. / Kimber, Wendy L.; Puri, Nitin; Borgmeyer, Carol; Ritter, Donna; Sharov, Alexei; Seidman, Michael; Ko, Minoru.
In: Reproductive BioMedicine Online, Vol. 6, No. 3, 04.2003, p. 318-322.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Efficacy of 2-methoxyethoxy-modified antisense oligonucleotides for the study of mouse preimplantation development
AU - Kimber, Wendy L.
AU - Puri, Nitin
AU - Borgmeyer, Carol
AU - Ritter, Donna
AU - Sharov, Alexei
AU - Seidman, Michael
AU - Ko, Minoru
PY - 2003/4
Y1 - 2003/4
N2 - The advent of microarray technology, coupled with the availability of mouse cDNA collections derived specifically from preimplantation embryos, helps to provide global gene expression profiles for the earliest stages of development. However, to determine the functions of the large numbers of genes of interest, massive systematic functional assays such as gene 'knockdown' experiments are required. As a first step, the relative suppression of blastocyst formation by differentially-modified antisense oligonucleotides to E-cadherin was assayed. The injection of 2′-methoxyethoxy (2′-MOE)-modified oligonucleotides blocked the formation of blastocysts in two-thirds of embryos, whereas the injection of either control missense 2′-MOE-oligonucleotides, or oligonucleotides with a Morpholino modification, had no significant effect on embryonic development. Thus, the 2′-MOE-modified antisense oligonucleotides are candidates for effective examination of roles of large numbers of genes during early embryological development.
AB - The advent of microarray technology, coupled with the availability of mouse cDNA collections derived specifically from preimplantation embryos, helps to provide global gene expression profiles for the earliest stages of development. However, to determine the functions of the large numbers of genes of interest, massive systematic functional assays such as gene 'knockdown' experiments are required. As a first step, the relative suppression of blastocyst formation by differentially-modified antisense oligonucleotides to E-cadherin was assayed. The injection of 2′-methoxyethoxy (2′-MOE)-modified oligonucleotides blocked the formation of blastocysts in two-thirds of embryos, whereas the injection of either control missense 2′-MOE-oligonucleotides, or oligonucleotides with a Morpholino modification, had no significant effect on embryonic development. Thus, the 2′-MOE-modified antisense oligonucleotides are candidates for effective examination of roles of large numbers of genes during early embryological development.
KW - Antisense oligonucleotide
KW - Embryo
KW - Gene 'knockdown'
KW - Preimplantation development
UR - http://www.scopus.com/inward/record.url?scp=0037899085&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037899085&partnerID=8YFLogxK
M3 - Article
VL - 6
SP - 318
EP - 322
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
SN - 1472-6483
IS - 3
ER -