Efficacy of New Polylactic Acid Nonwoven Fabric as a Hemostatic Agent in a Rat Liver Resection Model

Taiga Wakabayashi, Hiroshi Yagi, Kazuki Tajima, Kohei Kuroda, Masahiro Shinoda, Minoru Kitago, Yuta Abe, Go Oshima, Kazuya Hirukawa, Osamu Itano, Yuukou Kitagawa

Research output: Contribution to journalArticle

Abstract

Background. During minimally invasive surgery, efficient and nontoxic hemostats are important for difficult to access bleeding areas. Polylactic acid is an ecofriendly hemostatic agent and we aimed to evaluate the efficacy of a polylactic acid nonwoven fabric (PLAF) developed by Toray Industries, Inc, on liver hemostasis in a preclinical study. Materials and Methods. PLAF consists of both 1-µm diameter fibers and 100-µm diameter beaded fibers. Four rats were used, and 2 trough-shaped resections of the liver parenchyma were performed (n = 8 lobes). Immediately after the resection, PLAF (PLAF group: n = 4 lobes) or rayon gauze (Rayon group: n = 4 lobes) were applied on the resected plane and compressed manually. We compared the mean time to hemostasis and blood loss per lobe, as well as histological findings between the groups. Results. The PLAF group had a significantly shorter bleeding time (P =.006), and showed lower blood loss compared with the Rayon group (P =.076). Histopathological evaluation showed a large amount of beads on the liver surface in the PLAF group. Aggregated red blood cells evident by electron microscopy and von Willebrand factor immunofluorescence were seen surrounding the beads. The PLAF group showed significantly greater von Willebrand factor expression than the Rayon group (P =.004). Discussion. This new PLAF showed superior outcomes thanks to its unique characteristic of forming beaded nanofibers, and it has the potential to be an efficient hemostat in minimally invasive surgery in the human body.

Original languageEnglish
JournalSurgical Innovation
DOIs
Publication statusPublished - 2019 Jan 1

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Hemostatics
Liver
Minimally Invasive Surgical Procedures
von Willebrand Factor
Hemostasis
Nanofibers
poly(lactic acid)
Bleeding Time
Human Body
Fluorescent Antibody Technique
Electron Microscopy
Industry
Erythrocytes
Hemorrhage
rayon

Keywords

  • biomedical engineering
  • evidence-based medicine/surgery
  • hepatobiliary

ASJC Scopus subject areas

  • Surgery

Cite this

Efficacy of New Polylactic Acid Nonwoven Fabric as a Hemostatic Agent in a Rat Liver Resection Model. / Wakabayashi, Taiga; Yagi, Hiroshi; Tajima, Kazuki; Kuroda, Kohei; Shinoda, Masahiro; Kitago, Minoru; Abe, Yuta; Oshima, Go; Hirukawa, Kazuya; Itano, Osamu; Kitagawa, Yuukou.

In: Surgical Innovation, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Kuroda, Kohei

AU - Shinoda, Masahiro

AU - Kitago, Minoru

AU - Abe, Yuta

AU - Oshima, Go

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AU - Kitagawa, Yuukou

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