Efficient production of biologically active human prolactin in Escherichia coli

Yoshiki Hiraoka, Yasuhiro Nomata, Koichi Matsuo, Nobuyuki Tsubota, Kiyoo Tanabe, Toshio Fukasawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To obtain an adequate amount of human prolactin (hPRL) for elucidation of the structure-function relationship, we have expressed the hPRL cDNA in Escherichia coli (E. coli) by using a high-expression vector. The vector contained a chimeric gene encoding a fusion of protein A, a peptide sensitive to collagenase digestion and hPRL, which was inserted downstream of the right direction promotor of λ phage. The resulting protein fusion was purified through three column chromatographies of immunoglobulin G-linked Sepharose 4B, DEAE-5PW, and phenyl-5PW. In a typical experiment, a final sample with a purity of more than 80% was obtained with a recovery of more than 40% judged by enzyme-linked immunosorbent assay (ELISA). The fusion thus obtained was digested with collagenase, and protein reactive to anti-hPRL antibody was purified through phenyl-5PW column chromatography. The hPRL sample was found to be identical to authentic hPRL with respect to the amino acid composition and an N-terminal sequence of 20 residues, except that it contained an additional four amino acids at the N-terminal end. This peptide was presumed to be derived from the collagenase-target sequence. The hPRL thus obtained was found to be as active as the authentic hormone either immunologically judged by ELISA or biologically judged by the growth stimulatory effect on rat Nb2 lymphoma cells.

Original languageEnglish
Pages (from-to)147-154
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume81
Issue number1-3
DOIs
Publication statusPublished - 1991

Fingerprint

Prolactin
Escherichia coli
Collagenases
Immunosorbents
Column chromatography
Fusion reactions
Chromatography
Assays
Enzyme-Linked Immunosorbent Assay
Amino Acids
Peptides
Bacteriophages
Gene encoding
Staphylococcal Protein A
Enzymes
Sepharose
Rats
Digestion
Lymphoma
Proteins

Keywords

  • human
  • Nb lymphoma
  • Prolactin
  • Protein engineering

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Efficient production of biologically active human prolactin in Escherichia coli. / Hiraoka, Yoshiki; Nomata, Yasuhiro; Matsuo, Koichi; Tsubota, Nobuyuki; Tanabe, Kiyoo; Fukasawa, Toshio.

In: Molecular and Cellular Endocrinology, Vol. 81, No. 1-3, 1991, p. 147-154.

Research output: Contribution to journalArticle

Hiraoka, Yoshiki ; Nomata, Yasuhiro ; Matsuo, Koichi ; Tsubota, Nobuyuki ; Tanabe, Kiyoo ; Fukasawa, Toshio. / Efficient production of biologically active human prolactin in Escherichia coli. In: Molecular and Cellular Endocrinology. 1991 ; Vol. 81, No. 1-3. pp. 147-154.
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