EfrAB, an ABC Multidrug Efflux Pump in Enterococcus faecalis

Eun Woo Lee, M. Nazmul Huda, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

A DNA fragment responsible for resistance to antimicrobial agents was cloned from the chromosomal DNA of Enterococcus faecalis ATCC 29212 by using drug-hypersensitive mutant Escherichia coli KAM32 as a host cell. Cells of E. coli KAM32 harboring a recombinant plasmid (pAEF82) carrying the DNA fragment became resistant to many structurally unrelated antimicrobial agents, such as norfloxacin, ciprofloxacin, doxycycline, acriflavine, 4′ ,6-diamidino-2-phenylindole, tetraphenylphosphonium chloride, daunorubicin, and doxorubicin. Since the sequence of the whole genome of E. faecalis is known, we sequenced several portions of the DNA insert in plasmid pAEF82 and identified two open reading frames within the insert. We designated the genes efrA and efrB. A search of the deduced amino acid sequences of EfrA and EfrB revealed that they are similar to each other and that they belong to the ATP-binding cassette (ABC) family of multidrug efflux transporters. Transformed E. coli KAM32 cells harboring efrAB showed energy-dependent efflux of acriflavine. The efflux activity was inhibited by reserpine, verapamil, and sodium-o-vanadate, known inhibitors of ABC efflux pumps.

Original languageEnglish
Pages (from-to)3733-3738
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume47
Issue number12
DOIs
Publication statusPublished - 2003 Dec
Externally publishedYes

Fingerprint

Enterococcus faecalis
Acriflavine
Adenosine Triphosphate
DNA
Escherichia coli
Anti-Infective Agents
Plasmids
Norfloxacin
Daunorubicin
Vanadates
Doxycycline
Reserpine
Ciprofloxacin
Verapamil
Doxorubicin
Open Reading Frames
Amino Acid Sequence
Genome
Pharmaceutical Preparations
Genes

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Lee, E. W., Huda, M. N., Kuroda, T., Mizushima, T., & Tsuchiya, T. (2003). EfrAB, an ABC Multidrug Efflux Pump in Enterococcus faecalis. Antimicrobial Agents and Chemotherapy, 47(12), 3733-3738. https://doi.org/10.1128/AAC.47.12.3733-3738.2003

EfrAB, an ABC Multidrug Efflux Pump in Enterococcus faecalis. / Lee, Eun Woo; Huda, M. Nazmul; Kuroda, Teruo; Mizushima, Tohru; Tsuchiya, Tomofusa.

In: Antimicrobial Agents and Chemotherapy, Vol. 47, No. 12, 12.2003, p. 3733-3738.

Research output: Contribution to journalArticle

Lee, EW, Huda, MN, Kuroda, T, Mizushima, T & Tsuchiya, T 2003, 'EfrAB, an ABC Multidrug Efflux Pump in Enterococcus faecalis', Antimicrobial Agents and Chemotherapy, vol. 47, no. 12, pp. 3733-3738. https://doi.org/10.1128/AAC.47.12.3733-3738.2003
Lee, Eun Woo ; Huda, M. Nazmul ; Kuroda, Teruo ; Mizushima, Tohru ; Tsuchiya, Tomofusa. / EfrAB, an ABC Multidrug Efflux Pump in Enterococcus faecalis. In: Antimicrobial Agents and Chemotherapy. 2003 ; Vol. 47, No. 12. pp. 3733-3738.
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