Eggshell membrane powder ameliorates intestinal inflammation by facilitating the restitution of epithelial injury and alleviating microbial dysbiosis

Huijuan Jia, Manaka Hanate, Wanping Aw, Hideomi Itoh, Kenji Saito, Shoko Kobayashi, Satoshi Hachimura, Shinji Fukuda, Masaru Tomita, Yukio Hasebe, Hisanori Kato

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Gut microbiota is an essential factor in the shaping of intestinal immune system development and driving inflammation in inflammatory bowel disease (IBD). We report the effects and microbe-host interactions underlying an intervention using fine powder of eggshell membrane (ESM) against IBD. ESM attenuated lipopolysaccharide-induced inflammatory cytokine production and promoted the Caco-2 cell proliferation by up-regulating growth factors in vitro. In a murine model of dextran sodium sulphate-induced colitis, ESM significantly suppressed the disease activity index and colon shortening. These effects were associated with significant ameliorations of gene expressions of inflammatory mediators, intestinal epithelial cell proliferation, restitution-related factors and antimicrobial peptides. Multifaceted integrated omics analyses revealed improved levels of energy metabolism-related genes, proteins and metabolites. Concomitantly, cecal metagenomic information established an essential role of ESM in improving dysbiosis characterized by increasing the diversity of bacteria and decreasing absolute numbers of pathogenic bacteria such as Enterobacteriaceae and E. coli, as well as in the regulation of the expansion of Th17 cells by suppressing the overgrowth of segmented filamentous bacteria. Such modulations have functional effects on the host; i.e., repairing the epithelium, regulating energy requirements and eventually alleviating mucosal inflammation. These findings are first insights into ESM's modulation of microbiota and IBD suppression, providing new perspectives on the prevention/treatment of IBD.

Original languageEnglish
Article number43993
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 2017 Mar 8

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Dysbiosis
Egg Shell
Inflammatory Bowel Diseases
Powders
Inflammation
Membranes
Wounds and Injuries
Bacteria
Cell Proliferation
Th17 Cells
Metagenomics
Dextran Sulfate
Caco-2 Cells
Microbiota
Enterobacteriaceae
Colitis
Energy Metabolism
Lipopolysaccharides
Immune System
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • General

Cite this

Eggshell membrane powder ameliorates intestinal inflammation by facilitating the restitution of epithelial injury and alleviating microbial dysbiosis. / Jia, Huijuan; Hanate, Manaka; Aw, Wanping; Itoh, Hideomi; Saito, Kenji; Kobayashi, Shoko; Hachimura, Satoshi; Fukuda, Shinji; Tomita, Masaru; Hasebe, Yukio; Kato, Hisanori.

In: Scientific Reports, Vol. 7, 43993, 08.03.2017.

Research output: Contribution to journalArticle

Jia, Huijuan ; Hanate, Manaka ; Aw, Wanping ; Itoh, Hideomi ; Saito, Kenji ; Kobayashi, Shoko ; Hachimura, Satoshi ; Fukuda, Shinji ; Tomita, Masaru ; Hasebe, Yukio ; Kato, Hisanori. / Eggshell membrane powder ameliorates intestinal inflammation by facilitating the restitution of epithelial injury and alleviating microbial dysbiosis. In: Scientific Reports. 2017 ; Vol. 7.
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