EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells

Atsuhiro Nakajo, Shin ichiro Yoshimura, Hiroko Togawa, Masataka Kunii, Tomohiko Iwano, Ayaka Izumi, Yuria Noguchi, Ayako Watanabe, Ayako Goto, Toshiro Sato, Akihiro Harada

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The highly conserved Rab guanosine triphosphatase (GTPase) Rab8 plays a role in exocytosis toward the polarized plasma membrane in eukaryotic cells. In murine Rab8-deficient small intestine cells, apical proteins are missorted into lysosomes. In this study, we identified a novel Rab8-interacting protein complex containing an EH domain-binding protein 1-like 1 (EHBP1L1), Bin1/amphiphysin II, and dynamin. Biochemical analyses showed that EHBP1L1 directly bound to GTP-loaded Rab8 and Bin1. The spatial dependency of these complexes at the endocytic recycling compartment (ERC) was demonstrated through overexpression and knockdown experiments. EHBP1L1-or Bin1-depleted or dynamininhibited small intestine organoids significantly accumulated apical membrane proteins but not basolateral membrane proteins in lysosomes. Furthermore, in EHBP1L1-deficient mice, small intestine cells displayed truncated and sparse microvilli, suggesting that EHBP1L1 maintains the apical plasma membrane by regulating apical transport. In summary, our data demonstrate that EHBP1L1 links Rab8 and the Bin1-dynamin complex, which generates membrane curvature and excises the vesicle at the ERC for apical transport.

Original languageEnglish
Pages (from-to)297-306
Number of pages10
JournalJournal of Cell Biology
Volume212
Issue number3
DOIs
Publication statusPublished - 2016

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Carrier Proteins
Epithelial Cells
Small Intestine
Dynamins
Lysosomes
Membrane Proteins
Cell Membrane
Organoids
Transport Vesicles
Spatial Analysis
Guanosine
Exocytosis
Eukaryotic Cells
Microvilli
Guanosine Triphosphate
Proteins
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Nakajo, A., Yoshimura, S. I., Togawa, H., Kunii, M., Iwano, T., Izumi, A., ... Harada, A. (2016). EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells. Journal of Cell Biology, 212(3), 297-306. https://doi.org/10.1083/jcb.201508086

EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells. / Nakajo, Atsuhiro; Yoshimura, Shin ichiro; Togawa, Hiroko; Kunii, Masataka; Iwano, Tomohiko; Izumi, Ayaka; Noguchi, Yuria; Watanabe, Ayako; Goto, Ayako; Sato, Toshiro; Harada, Akihiro.

In: Journal of Cell Biology, Vol. 212, No. 3, 2016, p. 297-306.

Research output: Contribution to journalArticle

Nakajo, A, Yoshimura, SI, Togawa, H, Kunii, M, Iwano, T, Izumi, A, Noguchi, Y, Watanabe, A, Goto, A, Sato, T & Harada, A 2016, 'EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells', Journal of Cell Biology, vol. 212, no. 3, pp. 297-306. https://doi.org/10.1083/jcb.201508086
Nakajo, Atsuhiro ; Yoshimura, Shin ichiro ; Togawa, Hiroko ; Kunii, Masataka ; Iwano, Tomohiko ; Izumi, Ayaka ; Noguchi, Yuria ; Watanabe, Ayako ; Goto, Ayako ; Sato, Toshiro ; Harada, Akihiro. / EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells. In: Journal of Cell Biology. 2016 ; Vol. 212, No. 3. pp. 297-306.
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