Eighteen SSR markers for the Japanese clawed salamander, Onychodactylus japonicus, and cross-amplification in its congeners

Natsuhiko Yoshikawa; Nobuaki Nagata

doi:10.5358/hsj.36.153

Eighteen SSR markers for the Japanese clawed salamander, Onychodactylus japonicus, and cross-amplification in its congeners

Natsuhiko Yoshikawa, Nobuaki Nagata

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We developed 18 novel microsatellite markers for Onychodactylus japonicus using Illumina Miseq System, and tried cross-amplifications in three congeners. The number of alleles and expected heterozygosity at each locus in O. japonicus were 3-15 (mean=6.83) and 0.45-0.89 (mean=0.73), respectively. Cross-amplifications in congeners succeeded in 9-13 of 18 loci, and eight loci were consistently amplified across all four species. The markers described here will be useful not only for investigating phylogeography and fine-scale genetic structure, but also for estimating genetic diversity of endangered species or populations for conservation management.

Original language	English
Pages (from-to)	153-158
Number of pages	6
Journal	Current Herpetology
Volume	36
Issue number	2
DOIs	https://doi.org/10.5358/hsj.36.153
Publication status	Published - 2017 Aug
Externally published	Yes

Keywords

Conservation genetics
Genetic diversity
Microsatellite
Onychodactylus japonicus
Population genetics

ASJC Scopus subject areas

Animal Science and Zoology

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title = "Eighteen SSR markers for the Japanese clawed salamander, Onychodactylus japonicus, and cross-amplification in its congeners",

abstract = "We developed 18 novel microsatellite markers for Onychodactylus japonicus using Illumina Miseq System, and tried cross-amplifications in three congeners. The number of alleles and expected heterozygosity at each locus in O. japonicus were 3-15 (mean=6.83) and 0.45-0.89 (mean=0.73), respectively. Cross-amplifications in congeners succeeded in 9-13 of 18 loci, and eight loci were consistently amplified across all four species. The markers described here will be useful not only for investigating phylogeography and fine-scale genetic structure, but also for estimating genetic diversity of endangered species or populations for conservation management.",

keywords = "Conservation genetics, Genetic diversity, Microsatellite, Onychodactylus japonicus, Population genetics",

author = "Natsuhiko Yoshikawa and Nobuaki Nagata",

note = "Funding Information: We are also grateful to two anonymous reviewers for valuable comments on the earlier version of manuscript. All DNA samples used in this study are stored in the Center for Molecular Biodiversity Research, National Museum of Nature and Science, Tokyo. This work was supported by JSPS KAKENHI Grant Number JP26870320 and the Capital of Mother Nature Tadami Academic research project to NY. Publisher Copyright: {\textcopyright} 2017 by The Herpetological Society of Japan.",

year = "2017",

month = aug,

doi = "10.5358/hsj.36.153",

language = "English",

volume = "36",

pages = "153--158",

journal = "Current Herpetology",

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AU - Yoshikawa, Natsuhiko

AU - Nagata, Nobuaki

N1 - Funding Information: We are also grateful to two anonymous reviewers for valuable comments on the earlier version of manuscript. All DNA samples used in this study are stored in the Center for Molecular Biodiversity Research, National Museum of Nature and Science, Tokyo. This work was supported by JSPS KAKENHI Grant Number JP26870320 and the Capital of Mother Nature Tadami Academic research project to NY. Publisher Copyright: © 2017 by The Herpetological Society of Japan.

PY - 2017/8

Y1 - 2017/8

N2 - We developed 18 novel microsatellite markers for Onychodactylus japonicus using Illumina Miseq System, and tried cross-amplifications in three congeners. The number of alleles and expected heterozygosity at each locus in O. japonicus were 3-15 (mean=6.83) and 0.45-0.89 (mean=0.73), respectively. Cross-amplifications in congeners succeeded in 9-13 of 18 loci, and eight loci were consistently amplified across all four species. The markers described here will be useful not only for investigating phylogeography and fine-scale genetic structure, but also for estimating genetic diversity of endangered species or populations for conservation management.

AB - We developed 18 novel microsatellite markers for Onychodactylus japonicus using Illumina Miseq System, and tried cross-amplifications in three congeners. The number of alleles and expected heterozygosity at each locus in O. japonicus were 3-15 (mean=6.83) and 0.45-0.89 (mean=0.73), respectively. Cross-amplifications in congeners succeeded in 9-13 of 18 loci, and eight loci were consistently amplified across all four species. The markers described here will be useful not only for investigating phylogeography and fine-scale genetic structure, but also for estimating genetic diversity of endangered species or populations for conservation management.

KW - Conservation genetics

KW - Genetic diversity

KW - Microsatellite

KW - Onychodactylus japonicus

KW - Population genetics

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DO - 10.5358/hsj.36.153

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JO - Current Herpetology

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Eighteen SSR markers for the Japanese clawed salamander, Onychodactylus japonicus, and cross-amplification in its congeners

Abstract

Keywords

ASJC Scopus subject areas

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