Abstract
To evaluate effects of current smoking on the breakdown of lung elastin, we measured levels of elastinderived peptides (EDP) in unconcentrated bronchoalveolar lavage (BAL) fluid from 42 community-based older asymptomatic volunteers (60 ± 11 yr [mean ± SD]) and examined the relationships of the concentrations of EDP with immunologically detected neutrophil elastase (NE) bound with α1-proteinase inhibitor and with esterolytic activity against the NE-sensitive synthetic substrate, methoxysuccinyl-alanyl-alanyl-prolyl- valyl paranitroanilide (MEOSAAPVNA). The measurement of EDP levels was carried out by an indirect, competitive ELISA, using a sheep 19G fraction generated against insoluble human lung elastin as a primary antibody. EDP concentrations were significantly elevated in current smokers (n = 24) compared with age-matched noncurrent smokers (n = 18) (29.9 ± 3.5 [mean ± SE] versus 17.0 ± 1.8 ng/mg BAL fluid albumin, p < 0.01). We found weak but significant correlations of EDP levels with NE-α1-proteinase inhibitor complex (r = 0.38, p < 0.05) and with the esterolytic activity against the NE-sensitive synthetic substrate in BAL fluid (r = 0.65, p < 0.001). In addition, EDP levels in BAL fluid had a significant positive relationship with plasma cotinine concentrations (r = 0.53, p < 0.001), though not with pack-years of smoking (r = 0.1, NS). These data provide further evidence that the concentrations of EDP increase in BAL fluid from current smokers compared with noncurrent smokers and that enhanced breakdown of the lung elastin is associated with the increased load of NE in the lung.
| Original language | English |
|---|---|
| Pages (from-to) | 720-724 |
| Number of pages | 5 |
| Journal | American Journal of Respiratory and Critical Care Medicine |
| Volume | 154 |
| Issue number | 3 I |
| Publication status | Published - 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
Elastin-derived peptides and neutrophil elastase in bronchoalveolar lavage fluid. / Betsuyaku, Tomoko; Nishimura, Masaharu; Yoshioka, Aya; Takeyabu, Kimihiro; Miyamoto, Kenji; Kawakami, Yoshikazu.
In: American Journal of Respiratory and Critical Care Medicine, Vol. 154, No. 3 I, 1996, p. 720-724.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Elastin-derived peptides and neutrophil elastase in bronchoalveolar lavage fluid
AU - Betsuyaku, Tomoko
AU - Nishimura, Masaharu
AU - Yoshioka, Aya
AU - Takeyabu, Kimihiro
AU - Miyamoto, Kenji
AU - Kawakami, Yoshikazu
PY - 1996
Y1 - 1996
N2 - To evaluate effects of current smoking on the breakdown of lung elastin, we measured levels of elastinderived peptides (EDP) in unconcentrated bronchoalveolar lavage (BAL) fluid from 42 community-based older asymptomatic volunteers (60 ± 11 yr [mean ± SD]) and examined the relationships of the concentrations of EDP with immunologically detected neutrophil elastase (NE) bound with α1-proteinase inhibitor and with esterolytic activity against the NE-sensitive synthetic substrate, methoxysuccinyl-alanyl-alanyl-prolyl- valyl paranitroanilide (MEOSAAPVNA). The measurement of EDP levels was carried out by an indirect, competitive ELISA, using a sheep 19G fraction generated against insoluble human lung elastin as a primary antibody. EDP concentrations were significantly elevated in current smokers (n = 24) compared with age-matched noncurrent smokers (n = 18) (29.9 ± 3.5 [mean ± SE] versus 17.0 ± 1.8 ng/mg BAL fluid albumin, p < 0.01). We found weak but significant correlations of EDP levels with NE-α1-proteinase inhibitor complex (r = 0.38, p < 0.05) and with the esterolytic activity against the NE-sensitive synthetic substrate in BAL fluid (r = 0.65, p < 0.001). In addition, EDP levels in BAL fluid had a significant positive relationship with plasma cotinine concentrations (r = 0.53, p < 0.001), though not with pack-years of smoking (r = 0.1, NS). These data provide further evidence that the concentrations of EDP increase in BAL fluid from current smokers compared with noncurrent smokers and that enhanced breakdown of the lung elastin is associated with the increased load of NE in the lung.
AB - To evaluate effects of current smoking on the breakdown of lung elastin, we measured levels of elastinderived peptides (EDP) in unconcentrated bronchoalveolar lavage (BAL) fluid from 42 community-based older asymptomatic volunteers (60 ± 11 yr [mean ± SD]) and examined the relationships of the concentrations of EDP with immunologically detected neutrophil elastase (NE) bound with α1-proteinase inhibitor and with esterolytic activity against the NE-sensitive synthetic substrate, methoxysuccinyl-alanyl-alanyl-prolyl- valyl paranitroanilide (MEOSAAPVNA). The measurement of EDP levels was carried out by an indirect, competitive ELISA, using a sheep 19G fraction generated against insoluble human lung elastin as a primary antibody. EDP concentrations were significantly elevated in current smokers (n = 24) compared with age-matched noncurrent smokers (n = 18) (29.9 ± 3.5 [mean ± SE] versus 17.0 ± 1.8 ng/mg BAL fluid albumin, p < 0.01). We found weak but significant correlations of EDP levels with NE-α1-proteinase inhibitor complex (r = 0.38, p < 0.05) and with the esterolytic activity against the NE-sensitive synthetic substrate in BAL fluid (r = 0.65, p < 0.001). In addition, EDP levels in BAL fluid had a significant positive relationship with plasma cotinine concentrations (r = 0.53, p < 0.001), though not with pack-years of smoking (r = 0.1, NS). These data provide further evidence that the concentrations of EDP increase in BAL fluid from current smokers compared with noncurrent smokers and that enhanced breakdown of the lung elastin is associated with the increased load of NE in the lung.
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M3 - Article
C2 - 8810611
AN - SCOPUS:0029842065
VL - 154
SP - 720
EP - 724
JO - American Journal of Respiratory and Critical Care Medicine
JF - American Journal of Respiratory and Critical Care Medicine
SN - 1073-449X
IS - 3 I
ER -