Elastin-derived peptides and neutrophil elastase in bronchoalveolar lavage fluid

Tomoko Betsuyaku, Masaharu Nishimura, Aya Yoshioka, Kimihiro Takeyabu, Kenji Miyamoto, Yoshikazu Kawakami

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

To evaluate effects of current smoking on the breakdown of lung elastin, we measured levels of elastinderived peptides (EDP) in unconcentrated bronchoalveolar lavage (BAL) fluid from 42 community-based older asymptomatic volunteers (60 ± 11 yr [mean ± SD]) and examined the relationships of the concentrations of EDP with immunologically detected neutrophil elastase (NE) bound with α1-proteinase inhibitor and with esterolytic activity against the NE-sensitive synthetic substrate, methoxysuccinyl-alanyl-alanyl-prolyl- valyl paranitroanilide (MEOSAAPVNA). The measurement of EDP levels was carried out by an indirect, competitive ELISA, using a sheep 19G fraction generated against insoluble human lung elastin as a primary antibody. EDP concentrations were significantly elevated in current smokers (n = 24) compared with age-matched noncurrent smokers (n = 18) (29.9 ± 3.5 [mean ± SE] versus 17.0 ± 1.8 ng/mg BAL fluid albumin, p < 0.01). We found weak but significant correlations of EDP levels with NE-α1-proteinase inhibitor complex (r = 0.38, p < 0.05) and with the esterolytic activity against the NE-sensitive synthetic substrate in BAL fluid (r = 0.65, p < 0.001). In addition, EDP levels in BAL fluid had a significant positive relationship with plasma cotinine concentrations (r = 0.53, p < 0.001), though not with pack-years of smoking (r = 0.1, NS). These data provide further evidence that the concentrations of EDP increase in BAL fluid from current smokers compared with noncurrent smokers and that enhanced breakdown of the lung elastin is associated with the increased load of NE in the lung.

Original languageEnglish
Pages (from-to)720-724
Number of pages5
JournalAmerican journal of respiratory and critical care medicine
Volume154
Issue number3 I
DOIs
Publication statusPublished - 1996 Jan 1

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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