Elastin fiber accumulation in liver correlates with the development of hepatocellular carcinoma

Yutaka Yasui, Tokiya Abe, Masayuki Kurosaki, Mayu Higuchi, Yasuyuki Komiyama, Tsubasa Yoshida, Tsuguru Hayashi, Konomi Kuwabara, Kenta Takaura, Natsuko Nakakuki, Hitomi Takada, Nobuharu Tamaki, Shoko Suzuki, Hiroyuki Nakanishi, Kaoru Tsuchiya, Jun Itakura, Yuka Takahashi, Akinori Hashiguchi, Michiie Sakamoto, Namiki Izumi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background & Aims: The fibrosis stage, which is evaluated by the distribution pattern of collagen fibers, is a major predictor for the development of hepatocellular carcinoma (HCC) for patients with hepatitis C. Meanwhile, the role of elastin fibers has not yet been elucidated. The present study was conducted to determine the significance of quantifying both collagen and elastin fibers. Methods: We enrolled 189 consecutive patients with hepatitis C and advanced fibrosis. Using Elastica van Gieson-stained whole-slide images of pretreatment liver biopsies, collagen and elastin fibers were evaluated pixel by pixel (0.46 μm/pixel) using an automated computational method. Consequently, fiber amount and cumulative incidences of HCC within 3 years were analyzed. Results: There was a significant correlation between collagen and elastin fibers, whereas variation in elastin fiber was greater than in collagen fiber. Both collagen fiber (p = 0.008) and elastin fiber (p <0.001) were significantly correlated with F stage. In total, 30 patients developed HCC during follow-up. Patients who have higher elastin fiber (p = 0.002) in addition to higher collagen fiber (p = 0.05) showed significantly higher incidences of HCC. With regard to elastin fiber, this difference remained significant in F3 patients. Furthermore, for patients with a higher collagen fiber amount, higher elastin was a significant predictor for HCC development (p = 0.02). Conclusions: Computational analysis is a novel technique for quantification of fibers with the added value of conventional staging. Elastin fiber is a predictor for the development of HCC independently of collagen fiber and F stage.

Original languageEnglish
Article numbere0154558
JournalPLoS One
Volume11
Issue number4
DOIs
Publication statusPublished - 2016 Apr 1

Fingerprint

elastin
Elastin
hepatoma
Liver
Hepatocellular Carcinoma
collagen
Collagen
liver
Fibers
hepatitis C
Hepatitis C
fibrosis
Fibrosis
Pixels
incidence
Incidence
Rubber
value added
biopsy
pretreatment

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Yasui, Y., Abe, T., Kurosaki, M., Higuchi, M., Komiyama, Y., Yoshida, T., ... Izumi, N. (2016). Elastin fiber accumulation in liver correlates with the development of hepatocellular carcinoma. PLoS One, 11(4), [e0154558]. https://doi.org/10.1371/journal.pone.0154558

Elastin fiber accumulation in liver correlates with the development of hepatocellular carcinoma. / Yasui, Yutaka; Abe, Tokiya; Kurosaki, Masayuki; Higuchi, Mayu; Komiyama, Yasuyuki; Yoshida, Tsubasa; Hayashi, Tsuguru; Kuwabara, Konomi; Takaura, Kenta; Nakakuki, Natsuko; Takada, Hitomi; Tamaki, Nobuharu; Suzuki, Shoko; Nakanishi, Hiroyuki; Tsuchiya, Kaoru; Itakura, Jun; Takahashi, Yuka; Hashiguchi, Akinori; Sakamoto, Michiie; Izumi, Namiki.

In: PLoS One, Vol. 11, No. 4, e0154558, 01.04.2016.

Research output: Contribution to journalArticle

Yasui, Y, Abe, T, Kurosaki, M, Higuchi, M, Komiyama, Y, Yoshida, T, Hayashi, T, Kuwabara, K, Takaura, K, Nakakuki, N, Takada, H, Tamaki, N, Suzuki, S, Nakanishi, H, Tsuchiya, K, Itakura, J, Takahashi, Y, Hashiguchi, A, Sakamoto, M & Izumi, N 2016, 'Elastin fiber accumulation in liver correlates with the development of hepatocellular carcinoma', PLoS One, vol. 11, no. 4, e0154558. https://doi.org/10.1371/journal.pone.0154558
Yasui Y, Abe T, Kurosaki M, Higuchi M, Komiyama Y, Yoshida T et al. Elastin fiber accumulation in liver correlates with the development of hepatocellular carcinoma. PLoS One. 2016 Apr 1;11(4). e0154558. https://doi.org/10.1371/journal.pone.0154558
Yasui, Yutaka ; Abe, Tokiya ; Kurosaki, Masayuki ; Higuchi, Mayu ; Komiyama, Yasuyuki ; Yoshida, Tsubasa ; Hayashi, Tsuguru ; Kuwabara, Konomi ; Takaura, Kenta ; Nakakuki, Natsuko ; Takada, Hitomi ; Tamaki, Nobuharu ; Suzuki, Shoko ; Nakanishi, Hiroyuki ; Tsuchiya, Kaoru ; Itakura, Jun ; Takahashi, Yuka ; Hashiguchi, Akinori ; Sakamoto, Michiie ; Izumi, Namiki. / Elastin fiber accumulation in liver correlates with the development of hepatocellular carcinoma. In: PLoS One. 2016 ; Vol. 11, No. 4.
@article{48478f4e841a496e86601c0bc8140ee8,
title = "Elastin fiber accumulation in liver correlates with the development of hepatocellular carcinoma",
abstract = "Background & Aims: The fibrosis stage, which is evaluated by the distribution pattern of collagen fibers, is a major predictor for the development of hepatocellular carcinoma (HCC) for patients with hepatitis C. Meanwhile, the role of elastin fibers has not yet been elucidated. The present study was conducted to determine the significance of quantifying both collagen and elastin fibers. Methods: We enrolled 189 consecutive patients with hepatitis C and advanced fibrosis. Using Elastica van Gieson-stained whole-slide images of pretreatment liver biopsies, collagen and elastin fibers were evaluated pixel by pixel (0.46 μm/pixel) using an automated computational method. Consequently, fiber amount and cumulative incidences of HCC within 3 years were analyzed. Results: There was a significant correlation between collagen and elastin fibers, whereas variation in elastin fiber was greater than in collagen fiber. Both collagen fiber (p = 0.008) and elastin fiber (p <0.001) were significantly correlated with F stage. In total, 30 patients developed HCC during follow-up. Patients who have higher elastin fiber (p = 0.002) in addition to higher collagen fiber (p = 0.05) showed significantly higher incidences of HCC. With regard to elastin fiber, this difference remained significant in F3 patients. Furthermore, for patients with a higher collagen fiber amount, higher elastin was a significant predictor for HCC development (p = 0.02). Conclusions: Computational analysis is a novel technique for quantification of fibers with the added value of conventional staging. Elastin fiber is a predictor for the development of HCC independently of collagen fiber and F stage.",
author = "Yutaka Yasui and Tokiya Abe and Masayuki Kurosaki and Mayu Higuchi and Yasuyuki Komiyama and Tsubasa Yoshida and Tsuguru Hayashi and Konomi Kuwabara and Kenta Takaura and Natsuko Nakakuki and Hitomi Takada and Nobuharu Tamaki and Shoko Suzuki and Hiroyuki Nakanishi and Kaoru Tsuchiya and Jun Itakura and Yuka Takahashi and Akinori Hashiguchi and Michiie Sakamoto and Namiki Izumi",
year = "2016",
month = "4",
day = "1",
doi = "10.1371/journal.pone.0154558",
language = "English",
volume = "11",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "4",

}

TY - JOUR

T1 - Elastin fiber accumulation in liver correlates with the development of hepatocellular carcinoma

AU - Yasui, Yutaka

AU - Abe, Tokiya

AU - Kurosaki, Masayuki

AU - Higuchi, Mayu

AU - Komiyama, Yasuyuki

AU - Yoshida, Tsubasa

AU - Hayashi, Tsuguru

AU - Kuwabara, Konomi

AU - Takaura, Kenta

AU - Nakakuki, Natsuko

AU - Takada, Hitomi

AU - Tamaki, Nobuharu

AU - Suzuki, Shoko

AU - Nakanishi, Hiroyuki

AU - Tsuchiya, Kaoru

AU - Itakura, Jun

AU - Takahashi, Yuka

AU - Hashiguchi, Akinori

AU - Sakamoto, Michiie

AU - Izumi, Namiki

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Background & Aims: The fibrosis stage, which is evaluated by the distribution pattern of collagen fibers, is a major predictor for the development of hepatocellular carcinoma (HCC) for patients with hepatitis C. Meanwhile, the role of elastin fibers has not yet been elucidated. The present study was conducted to determine the significance of quantifying both collagen and elastin fibers. Methods: We enrolled 189 consecutive patients with hepatitis C and advanced fibrosis. Using Elastica van Gieson-stained whole-slide images of pretreatment liver biopsies, collagen and elastin fibers were evaluated pixel by pixel (0.46 μm/pixel) using an automated computational method. Consequently, fiber amount and cumulative incidences of HCC within 3 years were analyzed. Results: There was a significant correlation between collagen and elastin fibers, whereas variation in elastin fiber was greater than in collagen fiber. Both collagen fiber (p = 0.008) and elastin fiber (p <0.001) were significantly correlated with F stage. In total, 30 patients developed HCC during follow-up. Patients who have higher elastin fiber (p = 0.002) in addition to higher collagen fiber (p = 0.05) showed significantly higher incidences of HCC. With regard to elastin fiber, this difference remained significant in F3 patients. Furthermore, for patients with a higher collagen fiber amount, higher elastin was a significant predictor for HCC development (p = 0.02). Conclusions: Computational analysis is a novel technique for quantification of fibers with the added value of conventional staging. Elastin fiber is a predictor for the development of HCC independently of collagen fiber and F stage.

AB - Background & Aims: The fibrosis stage, which is evaluated by the distribution pattern of collagen fibers, is a major predictor for the development of hepatocellular carcinoma (HCC) for patients with hepatitis C. Meanwhile, the role of elastin fibers has not yet been elucidated. The present study was conducted to determine the significance of quantifying both collagen and elastin fibers. Methods: We enrolled 189 consecutive patients with hepatitis C and advanced fibrosis. Using Elastica van Gieson-stained whole-slide images of pretreatment liver biopsies, collagen and elastin fibers were evaluated pixel by pixel (0.46 μm/pixel) using an automated computational method. Consequently, fiber amount and cumulative incidences of HCC within 3 years were analyzed. Results: There was a significant correlation between collagen and elastin fibers, whereas variation in elastin fiber was greater than in collagen fiber. Both collagen fiber (p = 0.008) and elastin fiber (p <0.001) were significantly correlated with F stage. In total, 30 patients developed HCC during follow-up. Patients who have higher elastin fiber (p = 0.002) in addition to higher collagen fiber (p = 0.05) showed significantly higher incidences of HCC. With regard to elastin fiber, this difference remained significant in F3 patients. Furthermore, for patients with a higher collagen fiber amount, higher elastin was a significant predictor for HCC development (p = 0.02). Conclusions: Computational analysis is a novel technique for quantification of fibers with the added value of conventional staging. Elastin fiber is a predictor for the development of HCC independently of collagen fiber and F stage.

UR - http://www.scopus.com/inward/record.url?scp=84966349231&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84966349231&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0154558

DO - 10.1371/journal.pone.0154558

M3 - Article

C2 - 27128435

AN - SCOPUS:84966349231

VL - 11

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 4

M1 - e0154558

ER -