TY - GEN
T1 - Electric stress produces bilayer lipid membranes by exclusion of excessive oil layer
AU - Matsunaga, Y.
AU - Osaki, T.
AU - Misawa, N.
AU - Fujii, S.
AU - Kamiya, K.
AU - Miki, N.
AU - Takeuchi, S.
N1 - Publisher Copyright:
© 2017 IEEE.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/2/23
Y1 - 2017/2/23
N2 - This paper proposes a formation method for oil-layer-free bilayer lipid membranes (BLM) by using a compressive force of DC electric field. Using a droplet contact device previously developed, constant-voltage steps were applied to the pre-bilayer membrane and carefully controlled the electric stress in response to the membrane features, based on optical and current monitoring. We examined the pathways of the membrane formation and disruption, and discovered a qualitative protocol for oil-layer-free BLM formation. We succeeded in BLM formation using 1:1-mixture of hexadecane and n-decane as an oil example, which was hardly available for the former studies using the device. The method would contribute to produce vivo-like membranes appropriate for membrane protein functionalities.
AB - This paper proposes a formation method for oil-layer-free bilayer lipid membranes (BLM) by using a compressive force of DC electric field. Using a droplet contact device previously developed, constant-voltage steps were applied to the pre-bilayer membrane and carefully controlled the electric stress in response to the membrane features, based on optical and current monitoring. We examined the pathways of the membrane formation and disruption, and discovered a qualitative protocol for oil-layer-free BLM formation. We succeeded in BLM formation using 1:1-mixture of hexadecane and n-decane as an oil example, which was hardly available for the former studies using the device. The method would contribute to produce vivo-like membranes appropriate for membrane protein functionalities.
UR - http://www.scopus.com/inward/record.url?scp=85015757801&partnerID=8YFLogxK
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U2 - 10.1109/MEMSYS.2017.7863435
DO - 10.1109/MEMSYS.2017.7863435
M3 - Conference contribution
AN - SCOPUS:85015757801
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 433
EP - 435
BT - 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017
Y2 - 22 January 2017 through 26 January 2017
ER -