Electric stress produces bilayer lipid membranes by exclusion of excessive oil layer

Y. Matsunaga, T. Osaki, N. Misawa, S. Fujii, K. Kamiya, Norihisa Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper proposes a formation method for oil-layer-free bilayer lipid membranes (BLM) by using a compressive force of DC electric field. Using a droplet contact device previously developed, constant-voltage steps were applied to the pre-bilayer membrane and carefully controlled the electric stress in response to the membrane features, based on optical and current monitoring. We examined the pathways of the membrane formation and disruption, and discovered a qualitative protocol for oil-layer-free BLM formation. We succeeded in BLM formation using 1:1-mixture of hexadecane and n-decane as an oil example, which was hardly available for the former studies using the device. The method would contribute to produce vivo-like membranes appropriate for membrane protein functionalities.

Original languageEnglish
Title of host publication2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages433-435
Number of pages3
ISBN (Electronic)9781509050789
DOIs
Publication statusPublished - 2017 Feb 23
Event30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017 - Las Vegas, United States
Duration: 2017 Jan 222017 Jan 26

Other

Other30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017
CountryUnited States
CityLas Vegas
Period17/1/2217/1/26

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Matsunaga, Y., Osaki, T., Misawa, N., Fujii, S., Kamiya, K., Miki, N., & Takeuchi, S. (2017). Electric stress produces bilayer lipid membranes by exclusion of excessive oil layer. In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017 (pp. 433-435). [7863435] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2017.7863435