Electro-osmotic trapping and compression of single DNA molecules while passing through a nanopore

Hirohito Yamazaki, Takaha Mizuguchi, Keiko Esashika, Toshiharu Saiki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Complicated DNA molecular behaviors exist during translocation into a nanopore because their large and coiled structure needs to unwind. In this work, we investigated DNA translocation dynamics through a 200 nm pore using a fast photon counting system (FPCS). We found that the dwell time of the DNA molecules depends on the inverse of voltage (τ ∝ V-1.02) with a large constant term (∼1 ms). In other words, spherical fluorescence bead translocation involves electrophoresis as well as other additional factors. Our theoretical calculation suggested that one additional factor is electro-osmotic trapping associated with the instantaneous Brownian motion before and after translocation. Furthermore, compressed DNA molecular conformation was seen as a result of the increase of peak photon counts and the decrease of electrophoretic mobility with voltage. Our experiments showed that the polymers at the vicinity of a nanopore can be trapped and compressed, which is necessary to understand how to control the polymer translocation into a nanopore.

Original languageEnglish
Pages (from-to)5381-5388
Number of pages8
JournalAnalyst
Volume144
Issue number18
DOIs
Publication statusPublished - 2019 Sep 21

Fingerprint

Nanopores
translocation
trapping
DNA
compression
Photons
Molecules
Polymers
Molecular Conformation
Nucleic Acid Conformation
Electrophoretic mobility
polymer
Electrophoresis
Brownian movement
Electric potential
Brownian motion
Fluorescence
Conformations
electrokinesis
fluorescence

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Electro-osmotic trapping and compression of single DNA molecules while passing through a nanopore. / Yamazaki, Hirohito; Mizuguchi, Takaha; Esashika, Keiko; Saiki, Toshiharu.

In: Analyst, Vol. 144, No. 18, 21.09.2019, p. 5381-5388.

Research output: Contribution to journalArticle

Yamazaki, Hirohito ; Mizuguchi, Takaha ; Esashika, Keiko ; Saiki, Toshiharu. / Electro-osmotic trapping and compression of single DNA molecules while passing through a nanopore. In: Analyst. 2019 ; Vol. 144, No. 18. pp. 5381-5388.
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