@inbook{915811bb3460468f8c86a8f2dbfa7f05,
title = "Electrochemical biosensors combined with isothermal amplification for quantitative detection of nucleic acids",
abstract = "In recent years, various isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR). The integration of isothermal amplification with electrical or electrochemical devices has enabled high-throughput nucleic acid-based assays with high sensitivity. We performed solid-phase rolling circle amplification (RCA) on the surface of a Au electrode, and detected RCA products in situ using chronocoulometry (CC) with [Ru (NH3)6]3+as the signaling molecule. Detection sensitivity for DNA and a microRNA (miR-143) was 100 fM and 1 pM, respectively. Furthermore, we conducted potentiometric DNA detection using an ethidium ion (Et+)-selective electrode (Et+ISE) for real-time monitoring of isothermal DNA amplification by primer-generation RCA (PG-RCA). The Et+ISE potential enabled real-time monitoring of the PG-RCA reaction in the range of 10 nM–1 μM of initial target DNA. Devices based on these electrochemical techniques represent a new strategy for replacing conventional PCR for on-site detection of nucleic acids of viruses or microorganisms.",
keywords = "Biosensor, Chronocoulometry, Ion selective electrode, Isothermal nucleic acid amplification, MicroRNA, PCR",
author = "Miyuki Tabata and Bo Yao and Ayaka Seichi and Koji Suzuki and Yuji Miyahara",
note = "Funding Information: This study was supported in part by the Center of Innovation (COI) Program from the Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Japan Society for the Promotion of Science (JSPS), through the ?Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program)? initiated by the Council for Science and Technology Policy (CSTP); and by a JSPS Postdoctoral Fellowship for Foreign Researchers (No. P11355). Human blood samples were provided by Dr. Xuan Mu at the Institute of Basic Medical Sciences Chinese Academy of Medical Sciences (School of Basic Medical Science Peking Union Medical College), and this study was approved by the Chinese Academy of Medical Sciences Review Board on Human Rights Related to Human Experimentation (No. 001 2014). Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6911-1_10",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "135--151",
booktitle = "Methods in Molecular Biology",
}