@inbook{915811bb3460468f8c86a8f2dbfa7f05,
title = "Electrochemical biosensors combined with isothermal amplification for quantitative detection of nucleic acids",
abstract = "In recent years, various isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR). The integration of isothermal amplification with electrical or electrochemical devices has enabled high-throughput nucleic acid-based assays with high sensitivity. We performed solid-phase rolling circle amplification (RCA) on the surface of a Au electrode, and detected RCA products in situ using chronocoulometry (CC) with [Ru (NH3)6]3+as the signaling molecule. Detection sensitivity for DNA and a microRNA (miR-143) was 100 fM and 1 pM, respectively. Furthermore, we conducted potentiometric DNA detection using an ethidium ion (Et+)-selective electrode (Et+ISE) for real-time monitoring of isothermal DNA amplification by primer-generation RCA (PG-RCA). The Et+ISE potential enabled real-time monitoring of the PG-RCA reaction in the range of 10 nM–1 μM of initial target DNA. Devices based on these electrochemical techniques represent a new strategy for replacing conventional PCR for on-site detection of nucleic acids of viruses or microorganisms.",
keywords = "Biosensor, Chronocoulometry, Ion selective electrode, Isothermal nucleic acid amplification, MicroRNA, PCR",
author = "Miyuki Tabata and Bo Yao and Ayaka Seichi and Koji Suzuki and Yuji Miyahara",
year = "2017",
doi = "10.1007/978-1-4939-6911-1_10",
language = "English",
volume = "1572",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "135--151",
booktitle = "Methods in Molecular Biology",
}