Electrochemical detection of hydrogen peroxide at platinum-modified diamond electrodes for an application in melamine strip tests

Bakhadir Rismetov, Tribidasari A. Ivandini, Endang Saepudin, Yasuaki Einaga

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Electrochemical detection of hydrogen peroxide (H2O2) at a platinum-deposited boron doped diamond was studied for an application in melamine strip test. Deposition of Pt particles at a boron-doped diamond surface was performed using cyclic voltammetry method. The method was also used for electrochemical measurements. The oxidation-reduction peak currents of H 2O2 at + 0.6 V and - 0.1 V (vs. Ag/AgCl), respectively were found to be linear in the concentration range of 0.05-20 mM (R2 = 0.99). Linearity was also observed to be dependent on horseradish peroxidase (HRP) concentration in the constant concentration of H2O2. The method was then applied in immunochromatographic strip test for melamine detection to determine the activity of HRP labeled at melamine antibody. Taking an assumption that the activity of HRP was equivalent to melamine concentration, linearity of the current responses to the concentrations of standard melamine solutions in the range of 5-100 μg/ml could be achieved. Limit of detection of 0.51 μg/ml was estimated. The obtained results demonstrated that the designed biosensor for detection of H2O2 could be applied for the quantitative and selective detection of melamine.

Original languageEnglish
Pages (from-to)88-95
Number of pages8
JournalDiamond and Related Materials
Volume48
DOIs
Publication statusPublished - 2014 Sep

Keywords

  • Horseradish peroxidase
  • Hydrogen peroxide
  • Immunochromatographic strip test
  • Melamine
  • Pt-modified diamond

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Chemistry(all)
  • Mechanical Engineering
  • Materials Chemistry
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Electrochemical detection of hydrogen peroxide at platinum-modified diamond electrodes for an application in melamine strip tests'. Together they form a unique fingerprint.

  • Cite this