Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis

M. Tabata, H. Yang, F. Mannan, Y. Katayama, T. Goda, A. Matsumoto, A. Seichi, K. Suzuki, Y. Miyahara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Label-free miniaturized electrochemical devices have been developed to monitor the nucleic acid amplification. We successfully monitored released protons during amplification reaction in real-time manner using miniaturized pH sensors and demonstrated quantitative detection of nucleic acid. Because the sensitivity of ion-sensitive field effect transistor (ISFET) (-58.0 mV/pH) and iridium/ iridium oxide (Ir/IrOx) electrode (-57.4 mV/pH) as a pH sensor showed close values to theoretical value (-59.2 mV/pH), the combination between isothermal nucleic acid amplification and the electrochemical detection was compatible with potentially stable measurement. The proposed system would be cost-effective and portable, and potentially implemented in clinical use such as diagnosis of infectious disease and cancer.

Original languageEnglish
Title of host publication2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1553-1556
Number of pages4
ISBN (Print)9781479989553
DOIs
Publication statusPublished - 2015 Aug 5
Event18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
Duration: 2015 Jun 212015 Jun 25

Other

Other18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
CountryUnited States
CityAnchorage
Period15/6/2115/6/25

Keywords

  • Electrochemical real-time monitoring
  • ion-sensitive field effect transistor (ISFET)
  • iridium/iridium oxide (Ir/IrOx) electrode
  • isothermal nucleic acid amplification

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

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