Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis

M. Tabata, H. Yang, F. Mannan, Y. Katayama, T. Goda, A. Matsumoto, A. Seichi, K. Suzuki, Y. Miyahara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Label-free miniaturized electrochemical devices have been developed to monitor the nucleic acid amplification. We successfully monitored released protons during amplification reaction in real-time manner using miniaturized pH sensors and demonstrated quantitative detection of nucleic acid. Because the sensitivity of ion-sensitive field effect transistor (ISFET) (-58.0 mV/pH) and iridium/ iridium oxide (Ir/IrOx) electrode (-57.4 mV/pH) as a pH sensor showed close values to theoretical value (-59.2 mV/pH), the combination between isothermal nucleic acid amplification and the electrochemical detection was compatible with potentially stable measurement. The proposed system would be cost-effective and portable, and potentially implemented in clinical use such as diagnosis of infectious disease and cancer.

Original languageEnglish
Title of host publication2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1553-1556
Number of pages4
ISBN (Print)9781479989553
DOIs
Publication statusPublished - 2015 Aug 5
Event18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
Duration: 2015 Jun 212015 Jun 25

Other

Other18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
CountryUnited States
CityAnchorage
Period15/6/2115/6/25

Fingerprint

Nucleic acids
nucleic acids
quantitative analysis
pH sensors
Amplification
Iridium
Monitoring
Chemical analysis
Ion sensitive field effect transistors
iridium
Labels
Protons
sensors
infectious diseases
Electrodes
Oxides
field effect transistors
cancer
Costs
costs

Keywords

  • Electrochemical real-time monitoring
  • ion-sensitive field effect transistor (ISFET)
  • iridium/iridium oxide (Ir/IrOx) electrode
  • isothermal nucleic acid amplification

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Tabata, M., Yang, H., Mannan, F., Katayama, Y., Goda, T., Matsumoto, A., ... Miyahara, Y. (2015). Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 1553-1556). [7181234] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181234

Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis. / Tabata, M.; Yang, H.; Mannan, F.; Katayama, Y.; Goda, T.; Matsumoto, A.; Seichi, A.; Suzuki, K.; Miyahara, Y.

2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 1553-1556 7181234.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tabata, M, Yang, H, Mannan, F, Katayama, Y, Goda, T, Matsumoto, A, Seichi, A, Suzuki, K & Miyahara, Y 2015, Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis. in 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015., 7181234, Institute of Electrical and Electronics Engineers Inc., pp. 1553-1556, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Anchorage, United States, 15/6/21. https://doi.org/10.1109/TRANSDUCERS.2015.7181234
Tabata M, Yang H, Mannan F, Katayama Y, Goda T, Matsumoto A et al. Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 1553-1556. 7181234 https://doi.org/10.1109/TRANSDUCERS.2015.7181234
Tabata, M. ; Yang, H. ; Mannan, F. ; Katayama, Y. ; Goda, T. ; Matsumoto, A. ; Seichi, A. ; Suzuki, K. ; Miyahara, Y. / Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis. 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 1553-1556
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AU - Seichi, A.

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