Electron microscopic cytochemistry of pseudo-Chediak-Higashi granules in 5 cases of AML

W. Mizushima, M. Eguchi, H. Sakakibara, K. Sugita, T. Furukawa, M. Kanagawa, T. Suda, M. Yoshida, Y. Miura, J. Matsui

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Abstract

Blasts from 5 cases of AML with pseudo-Chediak-Higashi granules were examined ultrastructurally and histocytochemically using peroxidase, acid phosphatase, high iron diamine (HID) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) stainings. Pseudo-Chediak-Higashi granules, which appeared as vacuole-like inclusions by light microscopy, generally contained electron-lucent materials. All pseudo-Chediak-Higashi granules were, positive for peroxidase but some were negative for acid phosphatase. Pseudo-Chediak-Higashi granules were HID positive, indicating that they contained sulfated glycoconjugates. Glycogen-like particles were observed in the pseudo-Chediak-Higashi granules with the PA-TCH-SP method, as occasionally observed in granules in drug resistant ALL blasts. In conclusion, the contents of pseudo-Chediak-Higashi granules, which seems to be formed by fusion of small granules, differed from those of normal azurophillic granules.

Original languageEnglish
Pages (from-to)799-806
Number of pages8
Journal[Rinsho ketsueki] The Japanese journal of clinical hematology
Volume31
Issue number6
Publication statusPublished - 1990 Jun 1

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ASJC Scopus subject areas

  • Medicine(all)

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Mizushima, W., Eguchi, M., Sakakibara, H., Sugita, K., Furukawa, T., Kanagawa, M., Suda, T., Yoshida, M., Miura, Y., & Matsui, J. (1990). Electron microscopic cytochemistry of pseudo-Chediak-Higashi granules in 5 cases of AML. [Rinsho ketsueki] The Japanese journal of clinical hematology, 31(6), 799-806.