Electron microscopic cytochemistry of pseudo-Chediak-Higashi granules in 5 cases of AML

W. Mizushima, M. Eguchi, H. Sakakibara, K. Sugita, T. Furukawa, M. Kanagawa, T. Suda, M. Yoshida, Y. Miura, J. Matsui

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Abstract

Blasts from 5 cases of AML with pseudo-Chediak-Higashi granules were examined ultrastructurally and histocytochemically using peroxidase, acid phosphatase, high iron diamine (HID) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) stainings. Pseudo-Chediak-Higashi granules, which appeared as vacuole-like inclusions by light microscopy, generally contained electron-lucent materials. All pseudo-Chediak-Higashi granules were, positive for peroxidase but some were negative for acid phosphatase. Pseudo-Chediak-Higashi granules were HID positive, indicating that they contained sulfated glycoconjugates. Glycogen-like particles were observed in the pseudo-Chediak-Higashi granules with the PA-TCH-SP method, as occasionally observed in granules in drug resistant ALL blasts. In conclusion, the contents of pseudo-Chediak-Higashi granules, which seems to be formed by fusion of small granules, differed from those of normal azurophillic granules.

Original languageEnglish
Pages (from-to)799-806
Number of pages8
Journal[Rinsho ketsueki] The Japanese journal of clinical hematology
Volume31
Issue number6
Publication statusPublished - 1990 Jun

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Histocytochemistry
Diamines
Acid Phosphatase
Peroxidase
Iron
Electrons
Silver Staining
Glycoconjugates
Vacuoles
Glycogen
Microscopy
Light
Pharmaceutical Preparations
periodic acid-thiocarbohydrazide-silver proteinate

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Mizushima, W., Eguchi, M., Sakakibara, H., Sugita, K., Furukawa, T., Kanagawa, M., ... Matsui, J. (1990). Electron microscopic cytochemistry of pseudo-Chediak-Higashi granules in 5 cases of AML. [Rinsho ketsueki] The Japanese journal of clinical hematology, 31(6), 799-806.

Electron microscopic cytochemistry of pseudo-Chediak-Higashi granules in 5 cases of AML. / Mizushima, W.; Eguchi, M.; Sakakibara, H.; Sugita, K.; Furukawa, T.; Kanagawa, M.; Suda, T.; Yoshida, M.; Miura, Y.; Matsui, J.

In: [Rinsho ketsueki] The Japanese journal of clinical hematology, Vol. 31, No. 6, 06.1990, p. 799-806.

Research output: Contribution to journalArticle

Mizushima, W, Eguchi, M, Sakakibara, H, Sugita, K, Furukawa, T, Kanagawa, M, Suda, T, Yoshida, M, Miura, Y & Matsui, J 1990, 'Electron microscopic cytochemistry of pseudo-Chediak-Higashi granules in 5 cases of AML', [Rinsho ketsueki] The Japanese journal of clinical hematology, vol. 31, no. 6, pp. 799-806.
Mizushima W, Eguchi M, Sakakibara H, Sugita K, Furukawa T, Kanagawa M et al. Electron microscopic cytochemistry of pseudo-Chediak-Higashi granules in 5 cases of AML. [Rinsho ketsueki] The Japanese journal of clinical hematology. 1990 Jun;31(6):799-806.
Mizushima, W. ; Eguchi, M. ; Sakakibara, H. ; Sugita, K. ; Furukawa, T. ; Kanagawa, M. ; Suda, T. ; Yoshida, M. ; Miura, Y. ; Matsui, J. / Electron microscopic cytochemistry of pseudo-Chediak-Higashi granules in 5 cases of AML. In: [Rinsho ketsueki] The Japanese journal of clinical hematology. 1990 ; Vol. 31, No. 6. pp. 799-806.
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