TY - JOUR
T1 - Electron microscopic cytochemistry of pseudo-Chediak-Higashi granules in 5 cases of AML
AU - Mizushima, W.
AU - Eguchi, M.
AU - Sakakibara, H.
AU - Sugita, K.
AU - Furukawa, T.
AU - Kanagawa, M.
AU - Suda, T.
AU - Yoshida, M.
AU - Miura, Y.
AU - Matsui, J.
PY - 1990/6/1
Y1 - 1990/6/1
N2 - Blasts from 5 cases of AML with pseudo-Chediak-Higashi granules were examined ultrastructurally and histocytochemically using peroxidase, acid phosphatase, high iron diamine (HID) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) stainings. Pseudo-Chediak-Higashi granules, which appeared as vacuole-like inclusions by light microscopy, generally contained electron-lucent materials. All pseudo-Chediak-Higashi granules were, positive for peroxidase but some were negative for acid phosphatase. Pseudo-Chediak-Higashi granules were HID positive, indicating that they contained sulfated glycoconjugates. Glycogen-like particles were observed in the pseudo-Chediak-Higashi granules with the PA-TCH-SP method, as occasionally observed in granules in drug resistant ALL blasts. In conclusion, the contents of pseudo-Chediak-Higashi granules, which seems to be formed by fusion of small granules, differed from those of normal azurophillic granules.
AB - Blasts from 5 cases of AML with pseudo-Chediak-Higashi granules were examined ultrastructurally and histocytochemically using peroxidase, acid phosphatase, high iron diamine (HID) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) stainings. Pseudo-Chediak-Higashi granules, which appeared as vacuole-like inclusions by light microscopy, generally contained electron-lucent materials. All pseudo-Chediak-Higashi granules were, positive for peroxidase but some were negative for acid phosphatase. Pseudo-Chediak-Higashi granules were HID positive, indicating that they contained sulfated glycoconjugates. Glycogen-like particles were observed in the pseudo-Chediak-Higashi granules with the PA-TCH-SP method, as occasionally observed in granules in drug resistant ALL blasts. In conclusion, the contents of pseudo-Chediak-Higashi granules, which seems to be formed by fusion of small granules, differed from those of normal azurophillic granules.
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M3 - Article
C2 - 2214171
AN - SCOPUS:0025441844
SN - 0485-1439
VL - 31
SP - 799
EP - 806
JO - [Rinshō ketsueki] The Japanese journal of clinical hematology
JF - [Rinshō ketsueki] The Japanese journal of clinical hematology
IS - 6
ER -