Electrophysiological and histological effects on canine right atrium by photosensitization reaction under catheterization in vivo

M. Takahashi, E. Ogawa, S. Motohashi, T. Nakamura, H. Kawakami, N. Machida, A. Ito, T. Kimura, Seiji Takatsuki, K. Fukumoto, S. Miyoshi, Keiichi Fukuda, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We investigated electrophysiological and histological effect on canine anatomical isthmus in right atrium by photosensitization reaction (PR) of talaporfin sodium operated via a manipulative 7 Fr. laser catheter to establish a nonthermal tachyarrhythmia treatment. We continuously administrated talaporfin sodium via a left femoral vein to maintain photosensitizer concentration of 25-35 μg/ml in blood plasma, which is within the range of clinical use in human. Fifteen-minute after kickoff of the photosensitizer administration, a 663 nm laser was irradiated via the laser catheter for 30 s/point with irradiance of 10 W/cm2. After 17 times irradiations, a 23 ms delay of the electrical signal propagation along tricuspid annulus was observed. This result might demonstrate the acute electrical conduction delay induced by PR. The canine heart was extracted 10 days after PR and Azan staining specimen was histologically evaluated to investigate the myocardial damage by PR. The transmural fibrosis in anatomical isthmus was found. We demonstrated the PR-induced electrical conduction delay in acute phase in vivo using continuous photosensitizer dosed canine model, which maintain the clinical photosensitizer concentrat.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8565
DOIs
Publication statusPublished - 2013
EventPhotonic Therapeutics and Diagnostics IX - San Francisco, CA, United States
Duration: 2013 Feb 22013 Feb 7

Other

OtherPhotonic Therapeutics and Diagnostics IX
CountryUnited States
CitySan Francisco, CA
Period13/2/213/2/7

Fingerprint

catheterization
Photosensitivity Disorders
Photosensitizing Agents
Photosensitizers
Heart Atria
Catheterization
Canidae
isthmuses
Catheters
Lasers
Sodium
sodium
lasers
blood plasma
conduction
fibrosis
Femoral Vein
annuli
staining
Blood

Keywords

  • Acute electrical conduction block
  • Catheterization
  • Myocardium
  • Photosensitization reaction

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Takahashi, M., Ogawa, E., Motohashi, S., Nakamura, T., Kawakami, H., Machida, N., ... Arai, T. (2013). Electrophysiological and histological effects on canine right atrium by photosensitization reaction under catheterization in vivo. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8565). [85654A] https://doi.org/10.1117/12.2000619

Electrophysiological and histological effects on canine right atrium by photosensitization reaction under catheterization in vivo. / Takahashi, M.; Ogawa, E.; Motohashi, S.; Nakamura, T.; Kawakami, H.; Machida, N.; Ito, A.; Kimura, T.; Takatsuki, Seiji; Fukumoto, K.; Miyoshi, S.; Fukuda, Keiichi; Arai, Tsunenori.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8565 2013. 85654A.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Takahashi, M, Ogawa, E, Motohashi, S, Nakamura, T, Kawakami, H, Machida, N, Ito, A, Kimura, T, Takatsuki, S, Fukumoto, K, Miyoshi, S, Fukuda, K & Arai, T 2013, Electrophysiological and histological effects on canine right atrium by photosensitization reaction under catheterization in vivo. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8565, 85654A, Photonic Therapeutics and Diagnostics IX, San Francisco, CA, United States, 13/2/2. https://doi.org/10.1117/12.2000619
Takahashi M, Ogawa E, Motohashi S, Nakamura T, Kawakami H, Machida N et al. Electrophysiological and histological effects on canine right atrium by photosensitization reaction under catheterization in vivo. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8565. 2013. 85654A https://doi.org/10.1117/12.2000619
Takahashi, M. ; Ogawa, E. ; Motohashi, S. ; Nakamura, T. ; Kawakami, H. ; Machida, N. ; Ito, A. ; Kimura, T. ; Takatsuki, Seiji ; Fukumoto, K. ; Miyoshi, S. ; Fukuda, Keiichi ; Arai, Tsunenori. / Electrophysiological and histological effects on canine right atrium by photosensitization reaction under catheterization in vivo. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8565 2013.
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