Elevated Aqueous Cytokine Levels in Eyes With Ocular Surface Diseases

Naohiko Aketa, Takefumi Yamaguchi, Teru Asato, Yukari Yagi-Yaguchi, Terumasa Suzuki, Kazunari Higa, Toshihide Kurihara, Yoshiyuki Satake, Kazuo Tsubota, Jun Shimazaki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Purpose To evaluate cytokine and protein levels in the aqueous humor (AqH) of eyes with ocular surface diseases. Design Prospective consecutive case series. Methods This study includes 14 patients (aged 62.4 ± 13.7 years) with chronic-phase ocular surface diseases (4 with ocular cicatricial pemphigoid, 5 with chemical burns, 2 with a thermal burn, 2 with Stevens-Johnson syndrome, and 1 with exposure keratitis), 14 matched patients without ocular surface disease (controls with corneal scar), and 30 patients who underwent cataract surgery (healthy controls). AqH samples were collected at the beginning of surgery. AqH levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, monocyte chemotactic protein [MCP]-1, interferon [IFN]-α, IFN-γ, macrophage inflammatory protein [MIP]-1α, MIP-1β, P-selectin, E-selectin, soluble-intercellular adhesion molecule [s-ICAM]-1, tumor necrosis factor [TNF]-α, granulocyte-macrophage colony-stimulating factor [GM-CSF], IFN-γ-induced protein [IP]-10) were measured using multiplex beads immunoassays. Results The levels of IL-6, IL-10, IL-17A, GM-CSF, E-selectin, P-selectin, and s-ICAM in AqH were significantly elevated in eyes with ocular surface diseases (in pg/mL: 1696 ± 804, 4.0 ± 1.0, 24.3 ± 9.8, 26.0 ± 18.3, 5150 ± 1232, 13122 ± 7219, and 7914 ± 2813, respectively), compared to healthy controls (IL-6: 6.36 ± 0.94, P =.001; IL-10: 1.68 ± 0.04, P =.0006; IL-17A: 3.7 ± 0.2, P =.008; GM-CSF: 2.7 ± 0.3, P =.007; E-selectin: 2093 ± 37, P =.0001; P-selectin: 3658 ± 137, P =.0001; sICAM-1: 1397 ± 119, P =.008). The levels of IL-6, IL-17A, E-selectin, and P-selectin in AqH were significantly higher in eyes with ocular surface diseases compared to those with corneal scar (IL-6: 44.1 ± 15.0, P =.0077; IL-17A: 4.1 ± 0.7, P =.034; E-selectin: 2439 ± 302, P =.039; and P-selectin: 5673 ± 1553, P =.017). Conclusions Multiple AqH cytokine levels were elevated in chronic ocular surface diseases.

Original languageEnglish
Pages (from-to)42-51
Number of pages10
JournalAmerican Journal of Ophthalmology
Volume184
DOIs
Publication statusPublished - 2017 Dec 1

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Eye Diseases
Aqueous Humor
P-Selectin
E-Selectin
Interleukin-17
Interleukin-6
Cytokines
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-10
Interferons
Macrophage Inflammatory Proteins
Interleukin-1
Benign Mucous Membrane Pemphigoid
Chemical Burns
Stevens-Johnson Syndrome
Interleukin-13
Keratitis
Chemokine CCL2
Interleukins
Cell Adhesion Molecules

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Aketa, N., Yamaguchi, T., Asato, T., Yagi-Yaguchi, Y., Suzuki, T., Higa, K., ... Shimazaki, J. (2017). Elevated Aqueous Cytokine Levels in Eyes With Ocular Surface Diseases. American Journal of Ophthalmology, 184, 42-51. https://doi.org/10.1016/j.ajo.2017.09.029

Elevated Aqueous Cytokine Levels in Eyes With Ocular Surface Diseases. / Aketa, Naohiko; Yamaguchi, Takefumi; Asato, Teru; Yagi-Yaguchi, Yukari; Suzuki, Terumasa; Higa, Kazunari; Kurihara, Toshihide; Satake, Yoshiyuki; Tsubota, Kazuo; Shimazaki, Jun.

In: American Journal of Ophthalmology, Vol. 184, 01.12.2017, p. 42-51.

Research output: Contribution to journalArticle

Aketa, N, Yamaguchi, T, Asato, T, Yagi-Yaguchi, Y, Suzuki, T, Higa, K, Kurihara, T, Satake, Y, Tsubota, K & Shimazaki, J 2017, 'Elevated Aqueous Cytokine Levels in Eyes With Ocular Surface Diseases', American Journal of Ophthalmology, vol. 184, pp. 42-51. https://doi.org/10.1016/j.ajo.2017.09.029
Aketa N, Yamaguchi T, Asato T, Yagi-Yaguchi Y, Suzuki T, Higa K et al. Elevated Aqueous Cytokine Levels in Eyes With Ocular Surface Diseases. American Journal of Ophthalmology. 2017 Dec 1;184:42-51. https://doi.org/10.1016/j.ajo.2017.09.029
Aketa, Naohiko ; Yamaguchi, Takefumi ; Asato, Teru ; Yagi-Yaguchi, Yukari ; Suzuki, Terumasa ; Higa, Kazunari ; Kurihara, Toshihide ; Satake, Yoshiyuki ; Tsubota, Kazuo ; Shimazaki, Jun. / Elevated Aqueous Cytokine Levels in Eyes With Ocular Surface Diseases. In: American Journal of Ophthalmology. 2017 ; Vol. 184. pp. 42-51.
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abstract = "Purpose To evaluate cytokine and protein levels in the aqueous humor (AqH) of eyes with ocular surface diseases. Design Prospective consecutive case series. Methods This study includes 14 patients (aged 62.4 ± 13.7 years) with chronic-phase ocular surface diseases (4 with ocular cicatricial pemphigoid, 5 with chemical burns, 2 with a thermal burn, 2 with Stevens-Johnson syndrome, and 1 with exposure keratitis), 14 matched patients without ocular surface disease (controls with corneal scar), and 30 patients who underwent cataract surgery (healthy controls). AqH samples were collected at the beginning of surgery. AqH levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, monocyte chemotactic protein [MCP]-1, interferon [IFN]-α, IFN-γ, macrophage inflammatory protein [MIP]-1α, MIP-1β, P-selectin, E-selectin, soluble-intercellular adhesion molecule [s-ICAM]-1, tumor necrosis factor [TNF]-α, granulocyte-macrophage colony-stimulating factor [GM-CSF], IFN-γ-induced protein [IP]-10) were measured using multiplex beads immunoassays. Results The levels of IL-6, IL-10, IL-17A, GM-CSF, E-selectin, P-selectin, and s-ICAM in AqH were significantly elevated in eyes with ocular surface diseases (in pg/mL: 1696 ± 804, 4.0 ± 1.0, 24.3 ± 9.8, 26.0 ± 18.3, 5150 ± 1232, 13122 ± 7219, and 7914 ± 2813, respectively), compared to healthy controls (IL-6: 6.36 ± 0.94, P =.001; IL-10: 1.68 ± 0.04, P =.0006; IL-17A: 3.7 ± 0.2, P =.008; GM-CSF: 2.7 ± 0.3, P =.007; E-selectin: 2093 ± 37, P =.0001; P-selectin: 3658 ± 137, P =.0001; sICAM-1: 1397 ± 119, P =.008). The levels of IL-6, IL-17A, E-selectin, and P-selectin in AqH were significantly higher in eyes with ocular surface diseases compared to those with corneal scar (IL-6: 44.1 ± 15.0, P =.0077; IL-17A: 4.1 ± 0.7, P =.034; E-selectin: 2439 ± 302, P =.039; and P-selectin: 5673 ± 1553, P =.017). Conclusions Multiple AqH cytokine levels were elevated in chronic ocular surface diseases.",
author = "Naohiko Aketa and Takefumi Yamaguchi and Teru Asato and Yukari Yagi-Yaguchi and Terumasa Suzuki and Kazunari Higa and Toshihide Kurihara and Yoshiyuki Satake and Kazuo Tsubota and Jun Shimazaki",
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T1 - Elevated Aqueous Cytokine Levels in Eyes With Ocular Surface Diseases

AU - Aketa, Naohiko

AU - Yamaguchi, Takefumi

AU - Asato, Teru

AU - Yagi-Yaguchi, Yukari

AU - Suzuki, Terumasa

AU - Higa, Kazunari

AU - Kurihara, Toshihide

AU - Satake, Yoshiyuki

AU - Tsubota, Kazuo

AU - Shimazaki, Jun

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Purpose To evaluate cytokine and protein levels in the aqueous humor (AqH) of eyes with ocular surface diseases. Design Prospective consecutive case series. Methods This study includes 14 patients (aged 62.4 ± 13.7 years) with chronic-phase ocular surface diseases (4 with ocular cicatricial pemphigoid, 5 with chemical burns, 2 with a thermal burn, 2 with Stevens-Johnson syndrome, and 1 with exposure keratitis), 14 matched patients without ocular surface disease (controls with corneal scar), and 30 patients who underwent cataract surgery (healthy controls). AqH samples were collected at the beginning of surgery. AqH levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, monocyte chemotactic protein [MCP]-1, interferon [IFN]-α, IFN-γ, macrophage inflammatory protein [MIP]-1α, MIP-1β, P-selectin, E-selectin, soluble-intercellular adhesion molecule [s-ICAM]-1, tumor necrosis factor [TNF]-α, granulocyte-macrophage colony-stimulating factor [GM-CSF], IFN-γ-induced protein [IP]-10) were measured using multiplex beads immunoassays. Results The levels of IL-6, IL-10, IL-17A, GM-CSF, E-selectin, P-selectin, and s-ICAM in AqH were significantly elevated in eyes with ocular surface diseases (in pg/mL: 1696 ± 804, 4.0 ± 1.0, 24.3 ± 9.8, 26.0 ± 18.3, 5150 ± 1232, 13122 ± 7219, and 7914 ± 2813, respectively), compared to healthy controls (IL-6: 6.36 ± 0.94, P =.001; IL-10: 1.68 ± 0.04, P =.0006; IL-17A: 3.7 ± 0.2, P =.008; GM-CSF: 2.7 ± 0.3, P =.007; E-selectin: 2093 ± 37, P =.0001; P-selectin: 3658 ± 137, P =.0001; sICAM-1: 1397 ± 119, P =.008). The levels of IL-6, IL-17A, E-selectin, and P-selectin in AqH were significantly higher in eyes with ocular surface diseases compared to those with corneal scar (IL-6: 44.1 ± 15.0, P =.0077; IL-17A: 4.1 ± 0.7, P =.034; E-selectin: 2439 ± 302, P =.039; and P-selectin: 5673 ± 1553, P =.017). Conclusions Multiple AqH cytokine levels were elevated in chronic ocular surface diseases.

AB - Purpose To evaluate cytokine and protein levels in the aqueous humor (AqH) of eyes with ocular surface diseases. Design Prospective consecutive case series. Methods This study includes 14 patients (aged 62.4 ± 13.7 years) with chronic-phase ocular surface diseases (4 with ocular cicatricial pemphigoid, 5 with chemical burns, 2 with a thermal burn, 2 with Stevens-Johnson syndrome, and 1 with exposure keratitis), 14 matched patients without ocular surface disease (controls with corneal scar), and 30 patients who underwent cataract surgery (healthy controls). AqH samples were collected at the beginning of surgery. AqH levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, monocyte chemotactic protein [MCP]-1, interferon [IFN]-α, IFN-γ, macrophage inflammatory protein [MIP]-1α, MIP-1β, P-selectin, E-selectin, soluble-intercellular adhesion molecule [s-ICAM]-1, tumor necrosis factor [TNF]-α, granulocyte-macrophage colony-stimulating factor [GM-CSF], IFN-γ-induced protein [IP]-10) were measured using multiplex beads immunoassays. Results The levels of IL-6, IL-10, IL-17A, GM-CSF, E-selectin, P-selectin, and s-ICAM in AqH were significantly elevated in eyes with ocular surface diseases (in pg/mL: 1696 ± 804, 4.0 ± 1.0, 24.3 ± 9.8, 26.0 ± 18.3, 5150 ± 1232, 13122 ± 7219, and 7914 ± 2813, respectively), compared to healthy controls (IL-6: 6.36 ± 0.94, P =.001; IL-10: 1.68 ± 0.04, P =.0006; IL-17A: 3.7 ± 0.2, P =.008; GM-CSF: 2.7 ± 0.3, P =.007; E-selectin: 2093 ± 37, P =.0001; P-selectin: 3658 ± 137, P =.0001; sICAM-1: 1397 ± 119, P =.008). The levels of IL-6, IL-17A, E-selectin, and P-selectin in AqH were significantly higher in eyes with ocular surface diseases compared to those with corneal scar (IL-6: 44.1 ± 15.0, P =.0077; IL-17A: 4.1 ± 0.7, P =.034; E-selectin: 2439 ± 302, P =.039; and P-selectin: 5673 ± 1553, P =.017). Conclusions Multiple AqH cytokine levels were elevated in chronic ocular surface diseases.

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