Elevated levels of macrophage-stimulating protein in induced sputum of patients with bronchiectasis

Y. Takano, O. Sakamoto, M. Suga, T. Suda, M. Ando

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We recently showed that macrophage-stimulating protein (MSP), a serum protein homologous to hepatocyte growth factor, promotes ciliary motility by activating its receptor, RON, on the airway ciliated epithelium. To investigate the functional involvement of MSP and RON in bronchiectasis, in which mucociliary clearance (MCC) is impaired, first we examined RON expression on the bronchial ciliated epithelium of patients with bronchiectasis. We confirmed RON expression at the apical surface of bronchial ciliated epithelium of patients with bronchiectasis as well as those of normal human bronchus. Next, we examined whether MSP is present in sputum of patients with bronchiectasis and normal control subjects. By Western blotting, we found that half of the MSP in sputum is present as a biologically active α/β chain heterodimer (mature MSP). In addition, we found that the MSP concentrations in sputum were significantly elevated in patients with bronchiectasis (n=8; 16·8±3·0 ng ml-1) compared with normal controls (n=9; 8·4±2·4 ng ml-1; P <0·05). In contrast, the difference in concentrations of serum MSP (pro-MSP) was not significant between the two groups. These results indicate that (i) MSP is supplied to the airways and converted to a biologically active form, (ii) MSP is increased in patients with bronchiectasis compared with normal controls. Taken together, our findings suggest that increased MSP may be involved in compensation for impaired MCC in bronchiectasis.

Original languageEnglish
Pages (from-to)784-790
Number of pages7
JournalRespiratory Medicine
Volume94
Issue number8
DOIs
Publication statusPublished - 2000

Fingerprint

Bronchiectasis
Sputum
Mucociliary Clearance
Epithelium
macrophage stimulating protein
Hepatocyte Growth Factor
Bronchi
Blood Proteins
Western Blotting

Keywords

  • Bronchiectasis
  • Induced sputum
  • Macrophage-stimulating protein
  • Mucociliary clearance
  • RON

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Elevated levels of macrophage-stimulating protein in induced sputum of patients with bronchiectasis. / Takano, Y.; Sakamoto, O.; Suga, M.; Suda, T.; Ando, M.

In: Respiratory Medicine, Vol. 94, No. 8, 2000, p. 784-790.

Research output: Contribution to journalArticle

Takano, Y. ; Sakamoto, O. ; Suga, M. ; Suda, T. ; Ando, M. / Elevated levels of macrophage-stimulating protein in induced sputum of patients with bronchiectasis. In: Respiratory Medicine. 2000 ; Vol. 94, No. 8. pp. 784-790.
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