Elevated levels of pentraxin 3 in systemic sclerosis

Associations with vascular manifestations and defective vasculogenesis

Yuichiro Shirai, Yuka Okazaki, Yumiko Inoue, Yuichi Tamura, Hidekata Yasuoka, Tsutomu Takeuchi, Masataka Kuwana

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objective. To clarify the role of pentraxin 3 (PTX3), a multifunctional pattern recognition protein that can suppress fibroblast growth factor 2 (FGF-2), in systemic sclerosis (SSc)-related vasculopathy. Methods. We assessed 171 SSc patients and 19 age- and sex-matched healthy control subjects. Circulating PTX3 and FGF-2 levels were measured by enzyme immunoassay, and CD34+CD133+CD309+ endothelial progenitor cells (EPCs) were counted by flow cytometry. Correlations between PTX3 and FGF-2 and the presence or future development of vascular manifestations, including digital ulcers and pulmonary arterial hypertension (PAH), were identified by univariate and multivariate analysis. The effect of PTX3 on EPC differentiation was evaluated in proangiogenic cultures of mouse bone marrow cells in combination with colony formation assay. Results. Circulating PTX3 and FGF-2 levels were significantly higher in SSc patients than in healthy control subjects. PTX3 was elevated in SSc patients who had digital ulcers or PAH, while FGF-2 was reduced in SSc patients with PAH. Multivariate analysis identified elevated PTX3 as an independent parameter associated with the presence of digital ulcers and PAH, and PTX3 levels were a useful predictor of future occurrences of digital ulcers. Reduced FGF-2 was independently associated with the presence of PAH. EPC counts were significantly lower in patients with digital ulcers or PAH and correlated negatively with circulating PTX3 concentrations. Finally, PTX3 inhibited EPC differentiation in vitro. Conclusion. In SSc patients, exposure to high concentrations of PTX3 may suppress EPC-mediated vasculogenesis and promote vascular manifestations such as digital ulcers and PAH.

Original languageEnglish
Pages (from-to)498-507
Number of pages10
JournalArthritis and Rheumatology
Volume67
Issue number2
DOIs
Publication statusPublished - 2015 Feb 1

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Systemic Scleroderma
Blood Vessels
Pulmonary Hypertension
Fibroblast Growth Factor 2
Ulcer
Cell Differentiation
Healthy Volunteers
PTX3 protein
Multivariate Analysis
Immunoenzyme Techniques
Bone Marrow Cells
Flow Cytometry
Cell Count
Endothelial Progenitor Cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Rheumatology
  • Medicine(all)

Cite this

Elevated levels of pentraxin 3 in systemic sclerosis : Associations with vascular manifestations and defective vasculogenesis. / Shirai, Yuichiro; Okazaki, Yuka; Inoue, Yumiko; Tamura, Yuichi; Yasuoka, Hidekata; Takeuchi, Tsutomu; Kuwana, Masataka.

In: Arthritis and Rheumatology, Vol. 67, No. 2, 01.02.2015, p. 498-507.

Research output: Contribution to journalArticle

Shirai, Yuichiro ; Okazaki, Yuka ; Inoue, Yumiko ; Tamura, Yuichi ; Yasuoka, Hidekata ; Takeuchi, Tsutomu ; Kuwana, Masataka. / Elevated levels of pentraxin 3 in systemic sclerosis : Associations with vascular manifestations and defective vasculogenesis. In: Arthritis and Rheumatology. 2015 ; Vol. 67, No. 2. pp. 498-507.
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