Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA

George J. Kargul, Ramaiah Nagaraja, Tokihiko Shimada, Marija J. Grahovac, Meng K. Lim, Hiroshi Nakashima, Paul Waeltz, Peter Ma, Ellson Chen, David Schlessinger, Minoru Ko

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Targeted sequencing of the mouse t-complex has started with a 176-kb, gene-rich BAC localized with six PCR-based markers in inversion 2/3 of the highly duplicated region. The sequence contains 11 genes recovered primarily as cDNAs from early embryonic collections, including Igfals (previously placed on chromosome 17), Nubp2 (a fully characterized gene), Jsap1 (a JNK-binding protein), Rsp29 (the mouse homologue of the rat gene), Ndk3 (a nucleoside diphosphate kinase), and six additional putative genes of unknown function. With 50% GC content, 75% of the DNA transcribed, and one gene/16.0 kb (on average), the region may qualify as one of the most gene-dense segments in the mouse genome and provides candidates for dosage-sensitive phenotypes and mouse embryonic lethals mapped to the vicinity.

Original languageEnglish
Pages (from-to)916-923
Number of pages8
JournalGenome Research
Volume10
Issue number7
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

DNA
Genes
Nucleoside-Diphosphate Kinase
Chromosomes, Human, Pair 17
Base Composition
Carrier Proteins
Complementary DNA
Genome
Phenotype
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics

Cite this

Kargul, G. J., Nagaraja, R., Shimada, T., Grahovac, M. J., Lim, M. K., Nakashima, H., ... Ko, M. (2000). Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA. Genome Research, 10(7), 916-923. https://doi.org/10.1101/gr.10.7.916

Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA. / Kargul, George J.; Nagaraja, Ramaiah; Shimada, Tokihiko; Grahovac, Marija J.; Lim, Meng K.; Nakashima, Hiroshi; Waeltz, Paul; Ma, Peter; Chen, Ellson; Schlessinger, David; Ko, Minoru.

In: Genome Research, Vol. 10, No. 7, 2000, p. 916-923.

Research output: Contribution to journalArticle

Kargul, GJ, Nagaraja, R, Shimada, T, Grahovac, MJ, Lim, MK, Nakashima, H, Waeltz, P, Ma, P, Chen, E, Schlessinger, D & Ko, M 2000, 'Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA', Genome Research, vol. 10, no. 7, pp. 916-923. https://doi.org/10.1101/gr.10.7.916
Kargul GJ, Nagaraja R, Shimada T, Grahovac MJ, Lim MK, Nakashima H et al. Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA. Genome Research. 2000;10(7):916-923. https://doi.org/10.1101/gr.10.7.916
Kargul, George J. ; Nagaraja, Ramaiah ; Shimada, Tokihiko ; Grahovac, Marija J. ; Lim, Meng K. ; Nakashima, Hiroshi ; Waeltz, Paul ; Ma, Peter ; Chen, Ellson ; Schlessinger, David ; Ko, Minoru. / Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA. In: Genome Research. 2000 ; Vol. 10, No. 7. pp. 916-923.
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