Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA

George J. Kargul; Ramaiah Nagaraja; Tokihiko Shimada; Marija J. Grahovac; Meng K. Lim; Hiroshi Nakashima; Paul Waeltz; Peter Ma; Ellson Chen; David Schlessinger; Minoru S.H. Ko

doi:10.1101/gr.10.7.916

Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA

George J. Kargul, Ramaiah Nagaraja, Tokihiko Shimada, Marija J. Grahovac, Meng K. Lim, Hiroshi Nakashima, Paul Waeltz, Peter Ma, Ellson Chen, David Schlessinger, Minoru S.H. Ko

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Targeted sequencing of the mouse t-complex has started with a 176-kb, gene-rich BAC localized with six PCR-based markers in inversion 2/3 of the highly duplicated region. The sequence contains 11 genes recovered primarily as cDNAs from early embryonic collections, including Igfals (previously placed on chromosome 17), Nubp2 (a fully characterized gene), Jsap1 (a JNK-binding protein), Rsp29 (the mouse homologue of the rat gene), Ndk3 (a nucleoside diphosphate kinase), and six additional putative genes of unknown function. With 50% GC content, 75% of the DNA transcribed, and one gene/16.0 kb (on average), the region may qualify as one of the most gene-dense segments in the mouse genome and provides candidates for dosage-sensitive phenotypes and mouse embryonic lethals mapped to the vicinity.

Original language	English
Pages (from-to)	916-923
Number of pages	8
Journal	Genome Research
Volume	10
Issue number	7
DOIs	https://doi.org/10.1101/gr.10.7.916
Publication status	Published - 2000
Externally published	Yes

ASJC Scopus subject areas

Genetics
Genetics(clinical)

Access to Document

10.1101/gr.10.7.916

Cite this

@article{5c713c1c66904d8e9b2bdb8945a1863e,

title = "Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA",

abstract = "Targeted sequencing of the mouse t-complex has started with a 176-kb, gene-rich BAC localized with six PCR-based markers in inversion 2/3 of the highly duplicated region. The sequence contains 11 genes recovered primarily as cDNAs from early embryonic collections, including Igfals (previously placed on chromosome 17), Nubp2 (a fully characterized gene), Jsap1 (a JNK-binding protein), Rsp29 (the mouse homologue of the rat gene), Ndk3 (a nucleoside diphosphate kinase), and six additional putative genes of unknown function. With 50% GC content, 75% of the DNA transcribed, and one gene/16.0 kb (on average), the region may qualify as one of the most gene-dense segments in the mouse genome and provides candidates for dosage-sensitive phenotypes and mouse embryonic lethals mapped to the vicinity.",

author = "Kargul, {George J.} and Ramaiah Nagaraja and Tokihiko Shimada and Grahovac, {Marija J.} and Lim, {Meng K.} and Hiroshi Nakashima and Paul Waeltz and Peter Ma and Ellson Chen and David Schlessinger and Ko, {Minoru S.H.}",

year = "2000",

doi = "10.1101/gr.10.7.916",

language = "English",

volume = "10",

pages = "916--923",

journal = "Genome Research",

issn = "1088-9051",

publisher = "Cold Spring Harbor Laboratory Press",

number = "7",

}

TY - JOUR

T1 - Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA

AU - Kargul, George J.

AU - Nagaraja, Ramaiah

AU - Shimada, Tokihiko

AU - Grahovac, Marija J.

AU - Lim, Meng K.

AU - Nakashima, Hiroshi

AU - Waeltz, Paul

AU - Ma, Peter

AU - Chen, Ellson

AU - Schlessinger, David

AU - Ko, Minoru S.H.

PY - 2000

Y1 - 2000

N2 - Targeted sequencing of the mouse t-complex has started with a 176-kb, gene-rich BAC localized with six PCR-based markers in inversion 2/3 of the highly duplicated region. The sequence contains 11 genes recovered primarily as cDNAs from early embryonic collections, including Igfals (previously placed on chromosome 17), Nubp2 (a fully characterized gene), Jsap1 (a JNK-binding protein), Rsp29 (the mouse homologue of the rat gene), Ndk3 (a nucleoside diphosphate kinase), and six additional putative genes of unknown function. With 50% GC content, 75% of the DNA transcribed, and one gene/16.0 kb (on average), the region may qualify as one of the most gene-dense segments in the mouse genome and provides candidates for dosage-sensitive phenotypes and mouse embryonic lethals mapped to the vicinity.

AB - Targeted sequencing of the mouse t-complex has started with a 176-kb, gene-rich BAC localized with six PCR-based markers in inversion 2/3 of the highly duplicated region. The sequence contains 11 genes recovered primarily as cDNAs from early embryonic collections, including Igfals (previously placed on chromosome 17), Nubp2 (a fully characterized gene), Jsap1 (a JNK-binding protein), Rsp29 (the mouse homologue of the rat gene), Ndk3 (a nucleoside diphosphate kinase), and six additional putative genes of unknown function. With 50% GC content, 75% of the DNA transcribed, and one gene/16.0 kb (on average), the region may qualify as one of the most gene-dense segments in the mouse genome and provides candidates for dosage-sensitive phenotypes and mouse embryonic lethals mapped to the vicinity.

UR - http://www.scopus.com/inward/record.url?scp=0033859568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033859568&partnerID=8YFLogxK

U2 - 10.1101/gr.10.7.916

DO - 10.1101/gr.10.7.916

M3 - Article

C2 - 10899141

AN - SCOPUS:0033859568

SN - 1088-9051

VL - 10

SP - 916

EP - 923

JO - Genome Research

JF - Genome Research

IS - 7

ER -

Eleven densely clustered genes, six of them novel, in 176 kb of mouse t-complex DNA

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this