ELISA-CIEF using capillary-based microdevice: Highly-sensitive elisa based on capillary-isoelectric focusing of enzyme reaction product

Yuta Uenoyama, Ken Ikegami, Daniel Citterio, Koji Suzuki, Shun Ichi Funano, Terence G. Henares, Tatsuro Endo, Hideaki Hisamoto

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Sensitivity enhancement of enzyme-linked immunosorbent assay (ELISA) in capillary-based microdevice was achieved by the concentration of fluorescent final enzyme reaction product using capillary-isoelectric focusing (CIEF). In order to realize this, fluorescent substrate molecule, which can change its structure into ampholyte ion after an enzyme reaction, was newly synthesized and used. Approximately seven-fold enhancement of sensitivity and 1-2 orders of magnitude lower the detection limit were achieved.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1735-1737
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • Capillary isoelectric focusing
  • Enzyme-linked immunosorbent assay (ELISA)
  • Sensitivity enhancement
  • Square capillary

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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