TY - GEN
T1 - ELISA-CIEF using capillary-based microdevice
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
AU - Uenoyama, Yuta
AU - Ikegami, Ken
AU - Citterio, Daniel
AU - Suzuki, Koji
AU - Funano, Shun Ichi
AU - Henares, Terence G.
AU - Endo, Tatsuro
AU - Hisamoto, Hideaki
PY - 2012
Y1 - 2012
N2 - Sensitivity enhancement of enzyme-linked immunosorbent assay (ELISA) in capillary-based microdevice was achieved by the concentration of fluorescent final enzyme reaction product using capillary-isoelectric focusing (CIEF). In order to realize this, fluorescent substrate molecule, which can change its structure into ampholyte ion after an enzyme reaction, was newly synthesized and used. Approximately seven-fold enhancement of sensitivity and 1-2 orders of magnitude lower the detection limit were achieved.
AB - Sensitivity enhancement of enzyme-linked immunosorbent assay (ELISA) in capillary-based microdevice was achieved by the concentration of fluorescent final enzyme reaction product using capillary-isoelectric focusing (CIEF). In order to realize this, fluorescent substrate molecule, which can change its structure into ampholyte ion after an enzyme reaction, was newly synthesized and used. Approximately seven-fold enhancement of sensitivity and 1-2 orders of magnitude lower the detection limit were achieved.
KW - Capillary isoelectric focusing
KW - Enzyme-linked immunosorbent assay (ELISA)
KW - Sensitivity enhancement
KW - Square capillary
UR - http://www.scopus.com/inward/record.url?scp=84901755743&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901755743&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901755743
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1735
EP - 1737
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
Y2 - 28 October 2012 through 1 November 2012
ER -