ELISA measurement for urinary 3-hydroxyproline-containing peptides and its preliminary application to healthy persons and cancer patients

Junichi Saito, Yasutada Imamura, Joji Itoh, Shigeo Matsuyama, Akie Maruta, Toshihiko Hayashi, Ataru Sato, Norihito Wada, Kazuo Kashiwazaki, Yutaka Inagaki, Tetsu Watanabe, Yuko Kitagawa, Isao Okazaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background/Aim: We reported that endogenous urinary 3-hydroxyproline (3-Hyp) is useful for cancer screening because cancer invasion involves the destruction of basement membrane. A simple and sensitive assay is desired. Patients and Methods: An ELISA method using a specific antibody against a synthetic peptide of 10 amino acids including 3-Hyp corresponding to the amino acid sequences of collagen type IV alpha chain was applied to urine samples from 180 healthy controls and 22 cancer patients. Results: The values in controls were 2.44±1.90 (SD) mg peptide/g creatinine for 52 men and 2.87±2.01 for 128 women, while the values in 22 cancer patients were very low at 0.110±0.137 (p<0.001). Discussion: The discrepancy in the data between our previous and present studies is based on the difference of targets measured. 3-Hyp-containing peptides in cancer patients might be destroyed by the elevated peptidase levels found in these patients. Conclusion: This ELISA assay may be useful for cancer screening.

Original languageEnglish
Pages (from-to)1007-1014
Number of pages8
JournalAnticancer research
Volume30
Issue number3
Publication statusPublished - 2010 Mar 1

Keywords

  • 3-Hydroxyproline
  • Basement membrane
  • Cancer invasion
  • Cancer metastasis
  • Cancer screening
  • ELISA
  • Type IV collagen

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Saito, J., Imamura, Y., Itoh, J., Matsuyama, S., Maruta, A., Hayashi, T., Sato, A., Wada, N., Kashiwazaki, K., Inagaki, Y., Watanabe, T., Kitagawa, Y., & Okazaki, I. (2010). ELISA measurement for urinary 3-hydroxyproline-containing peptides and its preliminary application to healthy persons and cancer patients. Anticancer research, 30(3), 1007-1014.